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일개 대학병원의 혈액배양에서 MALDI-TOF MS를 이용한 Gram-positive Bacilli의 2년간 분기별 분리율

Two-year Quaternary Isolation of Gram-positive Bacilli Using MALDI-TOF MS in Positive Blood Culture of a University Hospital

김상하|김영권|원승호|유영빈|최진언
대한임상검사과학회 (Korean Society for Clinical Laboratory Science)|2018.12|대한임상검사과학회지 (Korean Journal of Clinical Laboratory Science)|제50권 제4호|pp. 414-421|DOI 10.15324/kjcls.2018.50.4.414

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참고문헌

키워드

MALDI-TOF MSBlood cultureIsolation rateGram positive bacilli
주제분류기타자연과학

초록

본 연구에서는 일개 대학병원에서 2년간 분리된 혈액배양 양성배지에서 MALDI-TOF MS system을 이용하여 Grampositive bacilli를 동정한 결과를 균종별, 분기별로 분석하였다. Corynebacterium striatum은 총 89균주 중 66균주(74.2%), Bacillus cereus는 60균주 중 44 균주 (73.3%), Listeria monocytogenes는 25균주 중 25균주 (100%)로 2.0 이상의 높은 스코어에서 동정되었다. 미 동정 된 균주는 303균주 중 293균주는 혈액배양에서 1회 분리 균주로 감염균으로서의 의의가 없는 오염 균주로 간주되었다. 감염균으로서 의의가있는 동일 환자 2회 이상 분리 균주 대상 16S-rRNA sequencing 비교결과 총 50균주 중 43균주가 일치해 86.0% 동정이 가능하였다. 일치하지 않은 7균주 중 5균주는 MALDI-TOF MS로도 동정이 되지 않았다. 결론적으로 혈액배양에서 Gram-positive bacilli가 동정되는 경우, 일차적으로 MALDI-TOF MS를 이용하여 동정해보고이를 활용한다면 어렵고 비용이 많이 들던 Gram-positive bacilli 동정이 저비용으로 더욱 간편하고 정확해지며, Grampositive bacilli에 의한 감염 진단에도 도움이 될 것으로 판단된다.

Abstract

In this study, Gram-positive bacilli (GPB) were identified by MALDI-TOF MS and analyzed according to the quaternary and microbial strains in the blood culture medium over a two year period at a university hospital. The results were as follows. The overall positive rate of blood culture was 9.97%. In 713 isolated GPB, 410 strains (57.5%) were identified using a microflex MALDI Biotyper. The positive rate of GPB among the blood culture positive bacteria was 8.2%, and the quarterly isolation rate was 9.8% in the third quarter of 2015, 8.7% in the second quarter of 2016, 8.1% in the third quarter of 2016, 8.1% in the first quarter of 2015, 7.9% in the first quarter of 2015, 7.9% in the second quarter of 2015, 6.8% in the first quarter of 2016, and 6.7% in the fourth quarter of 2015. The isolates were Corynebacterium striatum 89 (12.4%), Bacillus cereus 60 (8.4%), Bacillus subtilis 30 (4.2%), Paenibacillus urinalis 29 (4.1%), and Listeria monocytogenes 25 (3.5%). The results of 16S rRNA sequencing of 43 isolates (86.0%) were consistent with those of the other 50 isolates. Five out of the seven unmatched weeks were not identified by MALDI-TOF MS.

참고문헌 (21)

  1. Funke G, "Clinical microbiology of coryneform bacteria", Clin Microbiol Rev, 10, pp.125~159 (1997)
  2. Elisabeth E. Adderson, "Infections Caused by Coryneform Bacteria in Pediatric Oncology Patients", The Pediatric Infectious Disease Journal, 27, pp.136~141 (2008), Ovid Technologies (Wolters Kluwer Health)[DOI]
  3. Claudia Barberis, "Comparison of the Bruker MALDI-TOF Mass Spectrometry System and Conventional Phenotypic Methods for Identification of Gram-Positive Rods", PLoS ONE, 9(9), pp.e106303~ (2014), Public Library of Science (PLoS)[DOI]
  4. R. Scott Watson, "The Epidemiology of Severe Sepsis in Children in the United States", American Journal of Respiratory and Critical Care Medicine, 167(5), pp.695~701 (2003), American Thoracic Society[DOI]
  5. Holland RD, "Rapid identification of intact whole bacteria based on spectral patterns using matrix-assisted laser desorption", ionization with time-of-flight mass spectrometry, 1996, pp.10, 1227~1237
  6. Piseth Seng, "Ongoing Revolution in Bacteriology: Routine Identification of Bacteria by Matrix‐Assisted Laser Desorption Ionization Time‐of‐Flight Mass Spectrometry", Clinical Infectious Diseases, 49(4), pp.543~551 (2009), Oxford University Press (OUP)[DOI]
  7. A. Bizzini, "Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Bacterial Strains Routinely Isolated in a Clinical Microbiology Laboratory", Journal of Clinical Microbiology, 48(5), pp.1549~1554 (2010), American Society for Microbiology[DOI]
  8. L. G. Stevenson, "Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Clinically Important Yeast Species", Journal of Clinical Microbiology, 48(10), pp.3482~3486 (2010), American Society for Microbiology[DOI]
  9. 박강균, "혈액배양에서 VITEK MS와 VITEK 2 System을 이용한 신속 항생제 감수성 시험의 유용성 평가", 대한임상검사과학회지, 49(3), pp.279~284 (2017), 대한임상검사과학회[DOI]
  10. Etienne Carbonnelle, "Robustness of two MALDI-TOF mass spectrometry systems for bacterial identification", Journal of Microbiological Methods, 89(2), pp.133~136 (2012), Elsevier BV[DOI]
  11. D. Dubois, "Performances of the Vitek MS Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry System for Rapid Identification of Bacteria in Routine Clinical Microbiology", Journal of Clinical Microbiology, 50(8), pp.2568~2576 (2012), American Society for Microbiology[DOI]
  12. 안균열, "환자의 혈액배양에서 분리된 균종과 항균제 감수성의 변화추이", Annals of Clinical Microbiology, 9(1), pp.42~50 (2006), 대한임상미생물학회
  13. M. Navas, "Identification of Aerobic Gram-Positive Bacilli by Use of Vitek MS", Journal of Clinical Microbiology, 52(4), pp.1274~1277 (2014), American Society for Microbiology[DOI]
  14. 김낙현, "13년간 한 대학병원 혈액배양에서 분리된 균의 분포 및 항생제 감수성 변화 양상", Infection and Chemotherapy, 44(4), pp.275~281 (2012), 대한감염학회
  15. P.C.Y. Woo, "Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories", Clinical Microbiology and Infection, 14(10), pp.908~934 (2008), Elsevier BV[DOI]
  16. M. J. Bruins, "Identification and Susceptibility Testing of Enterobacteriaceae and Pseudomonas aeruginosa by Direct Inoculation from Positive BACTEC Blood Culture Bottles into Vitek 2", Journal of Clinical Microbiology, 42(1), pp.7~11 (2004), American Society for Microbiology[DOI]
  17. 신경섭, "Secular Trends of Species and Antimicrobial Resistance of Blood Isolates in a Tertiary Medical Center for Ten Years: 2003~2012", 대한의생명과학회지, 20(2), pp.77~84 (2014), 대한의생명과학회
  18. 최서경, "극소저체중출생아에서 폐 공기낭종이 합병된 Paenibacillus 패혈증 1례", Neonatal medicine, 21(1), pp.69~73 (2014), 대한신생아학회
  19. F. Renom, "Respiratory infection by Corynebacterium striatum: epidemiological and clinical determinants", New Microbes and New Infections, 2(4), pp.106~114 (2014), Elsevier BV[DOI]
  20. Cecilia Bittencourt Severo, "MULTIPLE PULMONARY NODULES CAUSED BY Corynebacterium striatum IN AN IMMUNOCOMPETENT PATIENT", Revista do Instituto de Medicina Tropical de São Paulo, 56(1), pp.89~91 (2014), FapUNIFESP (SciELO)[DOI]
  21. Simon Lévesque, "A Side by Side Comparison of Bruker Biotyper and VITEK MS: Utility of MALDI-TOF MS Technology for Microorganism Identification in a Public Health Reference Laboratory", PLOS ONE, 10(12), pp.e0144878~ (2015), Public Library of Science (PLoS)[DOI]

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