육계 농장에서 분리된 세균에 대한 222 nm Ultraviolet C 살균 효과 평가
Evaluation of 222 nm Ultraviolet C Bactericidal Efficacy on Bacterial Isolates from Broiler Farms
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초록
자외선(ultraviolet, UV)은 파장에 따라 분류되며, 254 nm UVC는 병원성 미생물의 DNA와 RNA를 손상시켜 강력한 살균효과를 나타내는 것으로 알려져 있다. 그러나 254 nm UVC는 인체 세포에 유해할 수 있어, 사람의 노출이 가능한 환경에서는 사용이 제한된다. 최근 222 nm UVC는 피부 표면을 통과하지 않아 인체에 대한 위험이 적으면서도 254 nm UVC와 유사한 항균 효과를 나타내어 기존 UVC보다 안전하게 활용할 수 있는 가능성이 제시되고 있다. 본 연구는 육계 농장의 깔짚과 분변에서 분리한 그람양성 세균으로 Mammaliicoccus lentus, Staphylococcus simulans와 그람음성 세균인 Pseudomonas putida, Proteus mirabilis, Escherichia coli에 대한 222 nm UVC의 살균 효과를 평가하였다. 222 nm UVC를 세균 현탁액에 0, 10, 30, 60, 120초 동안 조사한 결과, 모든 세균에서 생존율이 유의미하게 감소했으며, M. lentus를 제외한 모든 세균에서 60초 조사 후 완전히 사멸하였다. 이러한 결과는 222 nm UVC가 육계 농장과 같은 특수 환경에서 병원성 세균을 효과적으로 소독할 수 있음을 시사하며, 장기 노출의 안전성이 확인될 경우 다양한 축산 환경에서 소독 방법으로 활용될 가능성이 있다.
Abstract
Ultraviolet (UV) radiation is categorized by wavelengths 100∼400 nm, with 254 nm UVC having strong germicidal effects achieved by damaging the DNA and RNA of pathogenic microorganisms. On the other hand, the use of 254 nm UVC is restricted in environments where human exposure is possible because of its potential harm to human cells. Recently, 222 nm UVC has been suggested as a safer alternative because it does not penetrate the skin surface, posing less risk to humans while demonstrating antibacterial effects similar to those of 254 nm UVC. This study evaluated the bactericidal effects of 222 nm UVC on Gram-positive bacteria, Mammaliicoccus lentus and Staphylococcus simulans, and Gram-negative bacteria, Pseudomonas putida, Proteus mirabilis, and Escherichia coli, isolated from broiler farm litter and feces. Bacterial suspensions were exposed to 222 nm UVC for 0, 10, 30, 60, and 120 seconds. The results revealed a significant reduction (P＜ 0.001) in viability across all bacteria, with complete inactivation achieved in all species except for M. lentus after 60 seconds. These findings suggest that 222 nm UVC could be an effective disinfection method for pathogenic bacteria in specialized settings such as broiler farms, with potential applications in other livestock environments, pending confirmation of its safety for long-term exposure.
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