우분뇨 유래 젖소 유방염 저감을 위한 자외선 조사 살균의 효과 규명
Bacteriocidal Effects of Ultraviolet Irradiation for Reducing Bovine Mastitis Derived from Environmental Contamination
1
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Bovine mastitis is an important disease causing serious economic loss in dairy production and food poison in public health. The major causative agents of bovine mastitis include Escherichia coli (E. coli), Streptococcus agalactiae (S. agalactiae), Staphylococcus aureus (S. aureus). These bacteria were found in milk and environmental condition such as feces, water, soil and so on. Recently, many cases of mastitis are derived from environmental contamination of micro-organisms, which important factors for the spread of this disease in farm. Ultraviolet irradiation (UV) has been used as disinfection for waste and water in clinical and industrial facilities. Moreover the UV irradiation has been used as useful bactericidal agents to remove bacterial biofilms in environmental condition. In this study, we determined the bacterial replication in different percentage of water content (PWC) in sterilized saw dust and feces complexes from farm, and results showed that slightly decreased growth pattern of E. coli and S. agalactiae but increased growth pattern of S. aureus in various PWC (200, 400 and 600%) until 144 h incubation. In the bacteriocidal effect of UV irradiation to bacteria in saw dust and feces complex, the results showed that bacteriocidal effect was depended on the UV irradiation time, irradiation distance and PWC. Especially the antibacterial activity of UV irratiation is stronger in low PWC (50%), long time irradiation (50 sec), and short distance (5 cm) than other condition of this study. Furthermore UV irradiation with stirring showed increased the bactericidal effect compared without stirring. These results suggested that bovine mastitis causing agents may survive long time in environmental condition especially saw dust and feces complexes in farm and can cause a various disease including mastitis. Moreover, these data can be used as basis for application and development of UV disinfection to control of bovine mastitis from environmental contaminated bacteria in dairy farm.
Abstract
Bovine mastitis is an important disease causing serious economic loss in dairy production and food poison in public health. The major causative agents of bovine mastitis include Escherichia coli (E. coli), Streptococcus agalactiae (S. agalactiae), Staphylococcus aureus (S. aureus). These bacteria were found in milk and environmental condition such as feces, water, soil and so on. Recently, many cases of mastitis are derived from environmental contamination of micro-organisms, which important factors for the spread of this disease in farm. Ultraviolet irradiation (UV) has been used as disinfection for waste and water in clinical and industrial facilities. Moreover the UV irradiation has been used as useful bactericidal agents to remove bacterial biofilms in environmental condition. In this study, we determined the bacterial replication in different percentage of water content (PWC) in sterilized saw dust and feces complexes from farm, and results showed that slightly decreased growth pattern of E. coli and S. agalactiae but increased growth pattern of S. aureus in various PWC (200, 400 and 600%) until 144 h incubation. In the bacteriocidal effect of UV irradiation to bacteria in saw dust and feces complex, the results showed that bacteriocidal effect was depended on the UV irradiation time, irradiation distance and PWC. Especially the antibacterial activity of UV irratiation is stronger in low PWC (50%), long time irradiation (50 sec), and short distance (5 cm) than other condition of this study. Furthermore UV irradiation with stirring showed increased the bactericidal effect compared without stirring. These results suggested that bovine mastitis causing agents may survive long time in environmental condition especially saw dust and feces complexes in farm and can cause a various disease including mastitis. Moreover, these data can be used as basis for application and development of UV disinfection to control of bovine mastitis from environmental contaminated bacteria in dairy farm.
참고문헌 (22)
- Watts, J. L., "Etiological agents of bovine mastitis", Vet. Microbiol, 16, pp.41~66 (1988)
- Leclerc, H., "Advances in the bacteriology of the Coliform Group: Their suitability as markers of microbial water safety", Ann. Rev. Microbiol, 55, pp.201~234 (2001)
- Tallon, P., "Microbial indicators of faecal contamination in water: A current perspective", Water Air Soil Pollut, 166, pp.139~166 (2005)
- Van, Houdt R., "Role of bacterial cell surface structures in Escherichia coli biofilm formation", Res. Microbiol, 156, pp.626~633 (2005)
- Fox, L. K., "Fomites and reservoirs of Staphylococcus aureus causing intramammary infections as determined by phage typing: the effect of milking time hygiene practices", Cornell Vet, 81, pp.183~193 (1991)
- Zadoks, R. N., "Ribotyping of Streptococcus uberis from a dairy's environment, bovine feces and milk", Vet. Microbiol, 30;109(3-4), pp.257~265 (2005)
- Hogan, J. S., "Laboratory Handbook on Bovine Mastitis" (1999), National Mastitis Council, Inc.
- Koneman, E. W., "The Enterobacteriaceae, in: Color Atlas and Textbook of Diagnostic Microbiology", pp.57~124 (1983), J.B. Lippincott Company
- "Joe, H., K., Larry. S. (2003) Coliform mastitis. Vet. Res., 507–519", ~ (2003)
- Pore, R.S., "Prototheca ecology", Mycopathologia, 81, pp.49~62 (1983)
- Pore, R. S., "Prototheca zopfii: natural, transient, occurrence in pigs and rats", Mycopathologia, 101, pp.85~88 (1988)
- Decho, A. W., "Microbial biofilms in intertidal systems: An overview", Cont. Shelf. Res, 20, pp.1257~1273 (2000)
- Wilson, M., "Bactericidal effect of laser light and its potential use in the treatment of plaque-related diseases", Int. Dent. J, 44, pp.181~189 (1994)
- Nandakumar, K., "laser and UVA radiation impact on a PNP degrading Moraxella strain and its rpoS mutant", Biotechnol. Bioeng, 94, pp.793~802 (2006)
- Hamblin, M. R., "Helicobacter pylori accumulates photoactive porphyrins and is killed by visible light", Antimicrob. Agents Chemother, 49, pp.2822~2827 (2005)
- Giannini, M. S. H., "Suppression of pathogenesis in cutaneous Leishmaniasis by UV radiation", Infect. Immun, 51, pp.838~843 (1986)
- Mirando, W. S., "Ultraviolet- irradiated monocytes efficiently inhibit the intracellular replication of Mycobacterium avium intracellulare", J. Clin. Invest, 89(4), pp.1282~1287 (1992)
- Katara, G., "Surface disinfection by exposure to germicidal UV light", Indian J. Med. Microbiol, 26(3), pp.241~242 (2008)
- Hijnen, W. A., "Inactivation credit of UV radiation for viruses, bacteria and protozoan (oo)cysts in water: a review", Water Res, 40(1), pp.3~22 (2006)
- Lodish, H., "Molecular cell biology", pp.1344~ (1995), Scientific American Books, Inc.
- Becker, W. M., "The world of the cell", pp.802~ (2003), Benjamin Cummings
- Natasha, V., "The Bactericidal Effect of Ultraviolet and Visible Light on Escherichia coli", Biotechnol. Bioeng, 99(3), pp.550~556 (2008)