미토콘드리아 16S rDNA 부분 염기서열을 이용한 한국산 개구리 속 (Amphibia: Ranidae)의 종간, 종내 변이에 대한 연구
Intra-, Inter-specific Variation of Korean Rana(Amphibia: Ranidae) Based on the Partial Sequence of Mitochondrial 16S rDNA
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Abstract
- In order to clarify intra - and inter -specific variation of Korean Ranaspecies, the partial DNA sequences of mitochondrial 16S rDNA gene were determinedfrom 6 Korean and 1 Japanese Rana species. DNA sequences from Korean andJapanese species were comparison-analysed within, and also with the sequences fromthree species of Japanese brown frogs. DNA similarities were calculated as 91.3 ~ 97.3%among brown frog ( R. amurensis coreana , R. dybowskii and R. huanrenensis ), as 96.11~ 97.26% among pond frogs ( R. nigromaculata and R. plancyi chosenica ). Geneticdistance of pond frog and wrinkle frog ( R. rugosa ) were near than that of pond frogand brown frog. Two clusters were formed brown frogs and the other group by neigh-bor-joining and maximum -likelihood analysis, also the populations of R. nigroma-culata were well distinguished between Korean peninsula and Korean island. Butresult from maximum -likelihood analysis slightly differed from neighbor -joining tocluster of R. rugosa . Further analyses for their population will be necessary to studythe phylogenetic status.
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