제주도 숨은물벵뒤 습지 서식 Betaproteobacteria의 종다양성 및 신분류군 분포
Species Diversity of Betaproteobacteria in the Sumunmulbengdui Wetland Area of Jeju Island and Distribution of Novel Taxa
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제주도 숨은물벵뒤 습지에서 Betaproteobacteria의 종다양성을 조사하였고, 신분류군 후보 22균주를 확보하였다. 분리주의 16S rRNA 유전자 염기서열을 분석한 결과Burkholderiaceae (3균주), Comamonadaceae (8균주), Oxalobacteraceae (5균주) 및 Neisseriaceae (5균주) 등 4개 과에 속한 15속, 그리고 소속 미상의 Burkholderiales 소속1속(1균주)으로 동정되었다. Chromobacterium 속에 속한3종의 신분류군 후보를 확보하였고, Burkholderia, Comamonas,Pelomonas 및 Herbaspirillum의 4속에는 각각 2종씩, 그리고 나머지 속은 각각 1종씩의 신분류군 후보를 확보할 수 있었다. 이들 신분류군 후보에 대해 형태및 배양학적 특징의 분석과 더불어 막지방산 및 API 20NE 분석을 실시하여 22종의 신분류군 후보에 대한특징을 기술하였다.
Abstract
The species diversity of Betaproteobacteria in the Sumunmulbengdui Wetland Area of Jeju Island was studied using culture based techniques, and candidates for novel taxa were screened. Twenty two novel bacterial strains belonging to Betaproteobacteria were isolated, which could be assigned to 16 genera of 4 families, namely Burkholderiaceae (3 strains), Comamonadaceae (8strains), Oxalobacteraceae (5 strains), Neisseriaceae (5 strains), and an unassigned group belonging to Burkholderiales (1 strain) based 16S rRNA gene sequences. The genus Chromobacterium contained three candidates of novel species, and each of the genera Burkholderia, Comamonas, Pelomonas and Herbaspirillum contained two candidates respectively. Through the analysis of membrane fatty acid profiles and physiological properties using API 20NE as well as morphological and cultural properties, each of the isolates was found to form potentially novel species. Brief description of 22potential candidates for new species or subspecies is given accordingly.
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