제주도 숨은물벵뒤 습지 서식 Alphaproteobacteria 및 Gammaproteobacteria 강에 속하는 신변이주의 특성
Novel Taxa Belonging to the Class Alphaproteobacteria, and Gammaproteobacteria, Isolated from the Sumunmulbengdui Wetland Area of Jeju Island
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초록
제주도 한라산에 위치한 국내 최대규모의 고산습지인숨은물벵뒤 습지의 Alphaproteobacteria와 Gammaproteobacteria강에 속하는 세균 종의 다양성을 조사하였고,신분류군 후보 균주 19종을 확보하였다. 신종후보 균주의 16S rRNA 유전자 염기서열을 분석하였을 때 표준균주와 98.7% 미만의 유사도를 보이는 균주들을 신종후보균주로 간주하였다. 총 19종의 세균이 Alphaproteobacteria와Gammaproteobacteria 강에 속하는 신종후보 균주들로 발견되었다. Alphaproteobacteria 강에 속하는 균주들은 Sphingomonadaceae 과 Novosphingobium 속에 속하는 4종과 Rhizobiaceae 과 Rhizobium 속에 속하는 2종이분리되었다. Gammaproteobacteria 강에 속하는 균주들은Moraxellaceae 과 Acinetobacter 속에 속하는 2종, Pseudomonadaceae 과 Pseudomonas 속에 속하는 3종, Enterobacteriaceae 과의 Enterobacter 속에 속하는 1종, Erwinia 속의 2종, Leclercia 속의 1종, Rahnella 속의 1종, Serratia 속의 1종, Yersinia 속의 2종이 분리되었다. 분리된 19개후보신종에 대하여 배양학적 특징, 생리화학적 특징 및지방산 분석 결과를 정리하였다.
Abstract
In this study, samples were collected from the Sumummulbangdui wetland at the Halla Mountain in Jeju Island in order to isolate novel bacterial strain. Bacterial strains belonging to the class Alphaproteobacteria, and Gammaproteobacteria were isolated after spreading samples onto solid agar media. The 16S rRNA gene sequences of the strains assigned to the two classes were compared to those of type strains of the species. The strains that showed less than 98.7% 16S rRNA gene sequence similarity to the validly published species were considered to be novel species candidates. A total of 19 strains were regarded as novel strains which can be regarded as novel species candidates. In the Alphaproteobacteria, 6 novel strains were affiliated with the genera Novosphingobium,and Rhizobium. A total of 13 novel strains belong to Gammaproteobacteria that assigned to the family Moraxellaceae, Pseudomonadaceae, and Enterobacteriaceae were identified. Cultural,physiological, chemotaxonomic characteristics and fatty acids compositions have been determined for the novel species candidates, and the characteristics are described in this study.
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