갈조류 미역 유리배우체의 분리, 재생 및 성 숙 유도
Isolation, Regeneration and the Maturation Induction of Free-Living Gametophytes of Undaria pinnatifida (Phaeophyceae)
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초록
본 연구에서는 다양한 온도 (5, 10, 15 and 20℃), 조도 (5, 10, 20 and 40 μmol photons m-2 s-1) 및 광주기 (14 : 10, 12 : 12 and 10 : 14 h L : D) 조건에서 미역 배우체의 분리, 재생 및 성숙 조건을 파악하였다. 암배우체 단편은 15℃, 20 μmol photons m-2 s-1, 10 : 14 h (L : D) 조건에서 빠른 생장을 보인 반면 수배우체는 15℃, 5 μmol photons m-2 s-1, 14 : 10 h (L : D) 조건에서 빠른 생장을 보여 차이를 나타냈다. 암수배우체 의 최적 성숙 조건은 각각 5℃, 20~40 μmol photons m-2 s-1, 10 : 14 h (L : D) 조건과 15℃, 40 μmol photons m-2 s-1, 10 : 14 h (L : D) 조건이었다. 본 연구 결과 확인된 미역 유리배우 체의 재생과 성숙 조건은 미역의 형질개선과 교잡 육종 등을 위한 기초자료로 활용될 수 있다.
Abstract
The induction of regeneration and the maturation of the free-living gametophytes of Undaria pinnatifida were studied at temperatures (5, 10, 15 and 20℃), irradiance (5, 10, 20 and 40 μmol photons m-2 s-1) and photoperiods (14 : 10, 12 : 12 and 10 : 14 h L : D). Female gametophyte fragments were maintained in active regeneration without them reaching sexual maturity under conditions of 15℃, 20 μmol photons m-2 s-1, 10 : 14 h (L : D); by contrast, the conditions for male gametophytes were slightly different at 15℃, 5 μmol photons m-2 s-1, 14 : 10 h (L : D). The sexual maturation of female and male gametophytes took place under 5℃, 20-40 μmol photons m-2 s-1, 10 : 14 h (L : D) and 15℃, 40 μmol photons m-2 s-1, 10 : 14 h (L : D), respectively. These results provide basic information for controlling the regeneration and maturation of free-living gametophytes for srain improvement as well as the cross breeding of U. pinnatifida.
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