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갈조류 다시마 유리배우체의 분리, 재생 및 성 숙 유도

Isolation, Regeneration and Maturation Induction of Free-living Gametophytes of Saccharina japonica (Phaeophyceae)

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Induction of regeneration and maturation of the free-living gametophytes of Saccharina japonica was studied at four temperatures (5, 10, 15 and 20˚C), four levels of irradiance (5, 10, 20 and 40 μmol m-2 s-1) and three photoperiods (14 : 10, 12 : 12 and 10 : 14 h L : D). Female gametophyte fragments were maintained in active regeneration without reaching sexual maturity under conditions of 15˚C, 20 μmol m-2 s-1, 10 : 14 h (L : D), whereas the conditions for male gametophytes were slightly different at 15˚C, 5 μmol m-2 s-1, 14 : 10 h (L : D). The sexual maturation of female and male gametophytes took place under 5˚C, 20~40 μmol m-2 s-1, 10 : 14 h (L : D) and 15˚C, 40 μmol m-2 s-1, 10 : 14 h (L : D), respectively. These results provide basic information for controlling the regenerationand maturation of free-living gametophytes for strain improvement and cross breeding of S. japonica.

Abstract

Induction of regeneration and maturation of the free-living gametophytes of Saccharina japonica was studied at four temperatures (5, 10, 15 and 20˚C), four levels of irradiance (5, 10, 20 and 40 μmol m-2 s-1) and three photoperiods (14 : 10, 12 : 12 and 10 : 14 h L : D). Female gametophyte fragments were maintained in active regeneration without reaching sexual maturity under conditions of 15˚C, 20 μmol m-2 s-1, 10 : 14 h (L : D), whereas the conditions for male gametophytes were slightly different at 15˚C, 5 μmol m-2 s-1, 14 : 10 h (L : D). The sexual maturation of female and male gametophytes took place under 5˚C, 20~40 μmol m-2 s-1, 10 : 14 h (L : D) and 15˚C, 40 μmol m-2 s-1, 10 : 14 h (L : D), respectively. These results provide basic information for controlling the regenerationand maturation of free-living gametophytes for strain improvement and cross breeding of S. japonica.

참고문헌 (22)

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