불가사리 추출물의 복숭아혹진딧물(Myzus Persicae) 살충활성
Aphicidal Activity of Starfish (Asterina pectinifera) Extracts against Green Peach Aphid (Myzus Persicae)
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
본 연구에서는 불가사리 폐기물을 농업용 소재로서 활용 가능성을 조사하기 위해 불가사리 생체의 유기용매 추출물을 이용하여 복숭아혹진딧물 살충효율 실험을 수행하였다. 불가사리 추출물은 1,000 mg/L 수준에서 약 70% 이상의 살충효율을 보였으며 추출물을 정제하여 TOF-MS 분석한 결과 살충물질로서 polyhydroxysteroid가 동정되었다. Polyhydroxysteroid는 진딧물의 cuticle membrane을 파괴하여 진딧물을 살충하는 기작을 보유하고 있는 것으로 투과전자현미경 분석결과 밝혀졌다. 본 연구를 통해 폐기물 형태로 퇴치되고 있는 불가사리를 활용하면 진딧물 같은 해충방제용 소재로서 개발할 수 있을 것으로 기대되었다.
Abstract
BACKGROUND: Starfish is one of major sea invertebrates that have become a serious economic threat to aquacultural farms in Korea. Much effort has sacrificed to reduce the economic losses of the farms by predatory starfish, including developing and searching biological resources for medicinal and agricultural purposes. In the present study, we investigated aphicidal activity of the extracts from the starfish Asterina pectinifera against green peach aphid. METHODS AND RESULTS: Fresh starfishes were cut into small pieces, homogenized and soaked in methanol. The methanol extracts were centrifuged and the resulting supernatant was subjected to aphicidal activity assays and a series of silica gel column chromatography. More than 70% mortality of aphids were observed by the extracts at a concentration of 1,000 mg/L, exhibiting dose-dependent mortality. TOF-MS analyses detected polyhydroxysteroid as a main aphicidal compound from the starfish extracts. Transmission electronic microscopy could demonstrate that the extracts with polyhydroxysteroid caused aphids death by affecting their epicuticular membrane. CONCLUSION(s): This is the first report of aphicidal activity of the starfish Asterina pectinifera extracts against green peach aphid. Starfish biological resources may be used as a potential candidate for developing a new type natural insecticide.
참고문헌 (21)
- Abbott, W.S., "A method of computing the effectiveness of an insecticide", J. Eco. Entomol, 18, pp.265~267 (1925)
- Anderson, L, "Biological activity of saponins and saponin-like components from starfish and brittle-stars", Toxicon, 27, pp.179~188 (1989)
- Choi, G.H., "The effect of phytosphingosine isolated from Asterina pectinifera on cell damage induced by mite antigen in HaCaT cell and antibacterial activity against Staphylococcus aureus", AFRICAN JOURNAL OF BIOTECHNOLOGY, 9(6), pp.920~926 (2010), ACADEMIC JOURNALS
- de Marino, S., "McClintock, J.B., 1997. Isolation, structure elucidation, and biological activity of the steroid oligoglycosides and polyhydroxysteroids from the Antarctic starfish Acodontaster conspicuus", J. Nat. Prod, 60, pp.959~966 (1997)
- Ishii, T., "Aceramide and cerebroside from the starfish Asterias amurensis Lűtkne and their plant-growth promotion activities", J. Nat. Prod, 69, pp.1080~1082 (2006)
- Ishii, T., "Plant-growth regulators from common starfish (Asterias amurensis Lűtkne) waste", Plant Growth Regul, 52, pp.131~139 (2007)
- Kim, Y.S., "Selective feeding on the several bivalve mollusks by starfish, Asterias amurensis Lutken", Bull. Fac. Fish, 19, pp.244~249 (1969)
- 김영철, "Insecticidal activity of rhamnolipid Isolated from pseudomonas sp. EP-3 against green peach aphid (Myzus persicae)", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 59(3), pp.934~938, AMER CHEMICAL SOC
- Kang, K.H., "Predation of Asteiras amurensis and Asterina pectinifera on valuable bivalves at different water temperature", Korean J. Malacol, 16, pp.17~20 (2000)
- Lee, C.K., "Chemical components of starfish and their availability for fertilizer", Tech. Rep. Fish Res. Dev. Agency, 77, pp.57~74 (1989)
- Line,M.A., "Recycling of seastar (Asterias amurensis) waste by composting", Bioresour. Technol, 49, pp.227~229 (1994)
- Lveina, E.V., "Phrygisterol, a cytotoxic cyclopropane- containing polyhydroxysteroid, and related compounds from the pacific starfish Hippasteria phrygiana", J. Nat. Prod, 68, pp.1541~1544 (2005)
- Okano, K., "Structure of ovarian asterosaponin-4, an inhibitor of spontaneous oocyte maturation from the starfish Asterias amurensis", Agric. Biol. Chem, 49, pp.2823~2826 (1985)
- Paine, R.T., "A short term experimental investigation of resource partitioning in a New Zealand rocky intertidal habitat", Ecology, 52, pp.1096~1106 (1971)
- Palagiano, E., "Ptilomycalin A, crambescidin 800 and related new highly cytotoxic quanidine alkaloids from the starfishes Dermasterias imbricata", J. Am. Chem. Soc, 108, pp.8288~8289 (1995)
- Pathirana, C., "Imbrieatine, an unusual benzyltetrahydroisoquinoline alkaloid isolated from the starfish Dermasterias imbricata", J. Am. Chem. Soc, 108, pp.8288~8289 (1986)
- Prokof’eva, N.G., "Biological activities of steroid glycosides from starfish", Comp. Biochem. Physiol. B, 134, pp.695~701 (2003)
- Reccio, R, "Starfish saponins. Part 34. Novel steroidal glyxoside sulphates from the starfish Asterias amurensis", J. Chem. Soc. Perkin Trans, 1, pp.1337~1347 (1988)
- Tang, H.F., "A new asterosaponin from the starfish Culcita novaeguineae", Chinese Chem. Lett, 16, pp.619~622 (2005)
- Ukai, K., "Prevention by polyhydroxysteroids and saponins of Asterina pectinifera of the desquamation of stratum corneum cells", Biosci. Biotechnol. Biochem, 66, pp.913~915 (2002)
- 양유리, "Control of Green Peach Aphid (Myzus Persicae) by Combination of Plant Oil Formulations and Low-dosed Imidacloprid", 한국환경농학회지, 29(3), pp.239~246 (2010), 한국환경농학회