Physiological and Proteomics Analysis to Potassium Starvation in Rice
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
BACKGROUND:Potassium (K) is one of the macronutrients which are essential for plant growth and development. Its deficiency in paddy soils is becoming one of the limiting factors for increasing rice yield in Asia. METHODS AND RESULTS:To investigate physiological symptoms under K‐starvation (NP) compared with complete media (NPK) condition, we measured shoot/root length, weight, nutrients, and patterns of protein expression. The shoot growth was significantly reduced, but root growth was not affected by K‐starvation. However, biomasses were decreased in both shoot and root. Uptake of K was reduced up to 85%, while total concentrations of P, Ca, Mg, Na were increased in root and shoot. To better understand the starved K mechanism of rice, comparative proteome analysis for proteins isolated from rice leaves was conducted using 2‐DGE. Five spots of differentially expressed proteins were analyzed by MALDI‐TOF MS. Analysis of these K‐starvation response proteins suggested that they were involved in metabolism and defense. CONCLUSION(s):Physiological and 2‐DGE based proteomics approach used in our study results in observation of morphology or nutrients change and identification of K‐starvation responsive proteins in rice root. These results have important roles in maintaining nutrient homeostasis and would also be useful for further characterization of protein function in plant K nutrition.
Abstract
BACKGROUND:Potassium (K) is one of the macronutrients which are essential for plant growth and development. Its deficiency in paddy soils is becoming one of the limiting factors for increasing rice yield in Asia. METHODS AND RESULTS:To investigate physiological symptoms under K‐starvation (NP) compared with complete media (NPK) condition, we measured shoot/root length, weight, nutrients, and patterns of protein expression. The shoot growth was significantly reduced, but root growth was not affected by K‐starvation. However, biomasses were decreased in both shoot and root. Uptake of K was reduced up to 85%, while total concentrations of P, Ca, Mg, Na were increased in root and shoot. To better understand the starved K mechanism of rice, comparative proteome analysis for proteins isolated from rice leaves was conducted using 2‐DGE. Five spots of differentially expressed proteins were analyzed by MALDI‐TOF MS. Analysis of these K‐starvation response proteins suggested that they were involved in metabolism and defense. CONCLUSION(s):Physiological and 2‐DGE based proteomics approach used in our study results in observation of morphology or nutrients change and identification of K‐starvation responsive proteins in rice root. These results have important roles in maintaining nutrient homeostasis and would also be useful for further characterization of protein function in plant K nutrition.
참고문헌 (27)
- Adams, F., "Soil solution. In: The plant root and its environment", pp.441~481 (1971), University Press of Virginia
- Amtmann, A., "Potassium nutrition and salt stress. In membrane transport in plants", pp.293~339 (2004), Blackwell Publishing
- Armengaud, P., "The potassium dependent transcriptome of Arabidopsis reveals a prominent role of jasmonic acid in nutrient signalling", Plant Physiol, 136, pp.2556~2576 (2004)
- Ashley, M.K, "Plant responses to potassium deficiencies: a role for potassium transport proteins", J. Exp. Bot, 57, pp.425~436 (2006)
- Bednarz, C.W, "Leaf photosynthesis and carbon isotope discrimination of cotton in response to potassium deficiency", Environ. Exp. Bot, 39, pp.131~139 (1998)
- Chen, R.D., "Isolation of an osmotic stress‐and abscisic acid induced gene encoding an acidic endochitinase from Lycopersiconchilense", Mol. Genet. Genomics, 245, pp.195~202 (1994)
- Cho, K., "Survey of differentially expressed proteins and genes in jasmonic acid treated rice seedling shoot and root at the proteomics and transcriptomics levels", J. Proteome Res, 7, pp.2980~2998 (2007)
- Dixon, R.A, "Flavonoids and isoflavonoids―a gold mine for metabolic Engineering", Trends Plant Sci, 4, pp.394~400 (1999)
- Dhugga, K.S., "A reversibly glycosylated polypeptide (RGP1) possibly involved in plant cell wall synthesis: Purification, gene cloning, and trans‐Golgi localization", Proc. Natl. Acad. Sci. USA, 94, pp.7679~7684 (1997)
- Hong, J.K., "Promoter activation of pepper class II basic chitinase gene, CAChi2, and enhanced bacterial disease resistance and osmotic stress tolerance in the CAChi2-overexpressing Arabidopsis", PLANTA, 223(3), pp.433~448 (2006), Springer-Verlag
- Laegreid, M., "Agriculture, fertilizers and the environment" (1999), CABI
- Leigh, R.A., "A hypothesis relating critical potassium concentrations for growth to the distribution and function of this ion in the plant cell", New Phytologist, 97, pp.1~13 (1984)
- Marschner, H., "Mineral Nutrition of Higher Plants" (1995), Academic Press
- Mizuno, N, "The use of H2SO4‐H2O2 for the destruction of plants matter as a preliminary to determination of N, K, Mg, Ca, Fe, Mn", Jpn. J. Soil Sci. Plant Nutr, 51, pp.418~420 (1980)
- Karkonen, A., "UDPglucose dehydrogenase of maize: A role in cell wall pentose biosynthesis", Biochem. J, 391, pp.409~415 (2005)
- Kim, S.G, "Proteomic analysis of reactive oxygen species (ROS)-related proteins in rice roots", PLANT CELL REPORTS, 27(0), pp.363~375 (2008), SPRINGER
- Sang Gon Kim, "Physiological and proteomic analysis of young rice leaves grown under nitrogen-starvation conditions", Plant Biotechnology Reports, 5(4), pp.309~315 (2011), 한국식물생명공학회
- 김상곤, "Proteomic analysis of pathogen-responsive proteins from rice leaves induced by rice blast fungus, Magnaporthe grisea", PROTEOMICS, 4, pp.3569~3578, WILEY-V C H VERLAG GMBH
- Kim, S.T, "Proteomics analysis of rice lesion mimic mutant (spl1) reveals tightly localized probenazole induced protein (PBZ1) in cells undergoing programmed cell death", J. Proteome Res, 7, pp.1750~1760 (2008)
- Shin, R., "Hydrogen peroxide mediates plant root cell response to nutrient deprivation", Proc. Natl. Acad. Sci. USA, 101, pp.8827~8832 (2004)
- Tabuchi, M., "Severe reduction in growth rate and grain filling of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase1;1", Plant J, 42, pp.641~651 (2005)
- Véry, A.A., "Molecular mechanisms and regulation of K+ transport in higher plants", Annu. Rev. Plant Biol, 54, pp.575~603 (2003)
- Wasaki, J., "Transcriptomic analysis of metabolic changes by phosphorus stress in rice plant roots", Plant Cell Environ, 26, pp.1515~1523 (2003)
- Watanabe, T., "Distribution and chemical speciation of aluminum in the Al accumulator plant Melastoma malabathricum L", Plant Soil, 201, pp.165~173 (1998)
- Winkel‐Shirley, B., "Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology and biotechnology", Plant Physiol, 126, pp.485~493 (2001)
- Yan, S, "Proteomic analysis of salt stress‐responsive proteins in rice root", Proteomics, 5, pp.235~244 (2005)
- Zhao, D., "Influence of potassium deficiency on photosynthesis, chlorophyll content, and chloroplast ultrastructure of cotton plants", Photosynthetica, 39, pp.103~109 (2001)