Identification and Quantification of Glucosinolates in Rapeseed (Brassica napus L.) Sprouts Cultivated under Dark and Light Conditions
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BACKGROUND: This study was performed for theidentification and quantification of glucosinolate (GSL)contents in seven varieties of rapeseed (Brassica napus L.)sprouts cultivated under dark and light conditions. METHODS AND RESULTS: Crude glucosinolates(GSLs) were desulfated by treating with aryl sulfatase andpurified using diethylaminoethyl sepharose (DEAE) anionexchange column. Individual GSLs were quantified usinghigh-performance liquid chromatography (HPLC) withelectrospray ionization-tandem mass spectrometry (ESIMS/MS). Eleven GSLs including six aliphatic (progoitrin,sinigrin, glucoalyssin, gluconapoleiferin, gluconapin, andglucobrassicanapin), four indolyl (4-hydroxyglucobrassicin,glucobrassicin, 4-methoxyglucobrassicin, and neoglucobrassicin)and one aromatic (gluconasturtiin) were identified based onthe fragmentation patterns of MS spectrum. Aliphatic GSLswere noted as the predominant group with average 85.2% ofthe total contents. The most abundant GSLs were progoitrinwhich was ranged at 8.14–118.68 μmol/g dry weight (DW). The highest total GSL amounts were documented in ʻHanraʼ(146.02 μmol/g DW) under light condition and ‘Mokpo No. 68’ (86.67 μmol/g DW) in dark condition, whereas thelowest was in ʻTamraʼ (30.13 and 14.50 μmol/g DW) in bothconditions. The sum of aliphatic GSLs attributed > 80% inall varieties, except ʻTamraʼ (67.7% and 64.9% in dark andlight conditions, respectively) in the total GSLaccumulation. Indolyl GSLs were ranged 2.41–15.73μmol/g DW, accounted 2.78–33.6% of the total GSLs inrapeseed varieties. CONCLUSION(S): These results provide valuableinformation regarding potential beneficial GSL contentsindividually. This study attempts to contribute toknowledge of the nutritional properties of the differentvarieties of rapeseed plants. These results may be useful forthe evaluation of dietary information.
Abstract
BACKGROUND: This study was performed for theidentification and quantification of glucosinolate (GSL)contents in seven varieties of rapeseed (Brassica napus L.)sprouts cultivated under dark and light conditions. METHODS AND RESULTS: Crude glucosinolates(GSLs) were desulfated by treating with aryl sulfatase andpurified using diethylaminoethyl sepharose (DEAE) anionexchange column. Individual GSLs were quantified usinghigh-performance liquid chromatography (HPLC) withelectrospray ionization-tandem mass spectrometry (ESIMS/MS). Eleven GSLs including six aliphatic (progoitrin,sinigrin, glucoalyssin, gluconapoleiferin, gluconapin, andglucobrassicanapin), four indolyl (4-hydroxyglucobrassicin,glucobrassicin, 4-methoxyglucobrassicin, and neoglucobrassicin)and one aromatic (gluconasturtiin) were identified based onthe fragmentation patterns of MS spectrum. Aliphatic GSLswere noted as the predominant group with average 85.2% ofthe total contents. The most abundant GSLs were progoitrinwhich was ranged at 8.14–118.68 μmol/g dry weight (DW). The highest total GSL amounts were documented in ʻHanraʼ(146.02 μmol/g DW) under light condition and ‘Mokpo No. 68’ (86.67 μmol/g DW) in dark condition, whereas thelowest was in ʻTamraʼ (30.13 and 14.50 μmol/g DW) in bothconditions. The sum of aliphatic GSLs attributed > 80% inall varieties, except ʻTamraʼ (67.7% and 64.9% in dark andlight conditions, respectively) in the total GSLaccumulation. Indolyl GSLs were ranged 2.41–15.73μmol/g DW, accounted 2.78–33.6% of the total GSLs inrapeseed varieties. CONCLUSION(S): These results provide valuableinformation regarding potential beneficial GSL contentsindividually. This study attempts to contribute toknowledge of the nutritional properties of the differentvarieties of rapeseed plants. These results may be useful forthe evaluation of dietary information.
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