배추 유묘의 글루코시놀레이트 합성 기작에 미치는 LED 혼합광의 효과
Effect of LED mixed light conditions on the glucosinolate pathway in brassica rapa
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
In the agricultural industries, LEDs are used as supplementary, as well as main lighting sources in closed cultivation systems. In cultivation using artificial light sources, various light qualities have been tried to supplement fluorescent lamps to promote plant growth and metabolism. Microarray analysis of Brassica rapa seedlings under blue and fluorescent mixed with blue light conditions identified changes in three genes of the glucosinolate pathway. This attracted attention as functional materials highly expressed 3.6-4.6 fold under latter condition. We selected four more genes of the glucosinolate pathway from the Brassica database and tested their expression changes under fluorescent light mixed with red, green, and blue, respectively. Some genes increased expression under red and blue mixed conditions. The Bra026058, Bra015379, and Bra021429; the orthologous genes of CYP79F1, ST5a, and FMOGS-OX1 in Arabidopsis, are highly expressed in Brassica rapa under fluorescent mixed with blue light conditions. Further, Bra029355, Bra034180, Bra024634, and Bra022448; the orthologous genes of MAM1, AOP3, UGT74B1, and BCAT4 in Arabidopsis, are highly expressed in Brassica rapa under fluorescent mixed with red light conditions. The various light conditions had unique effects on the varieties of Brassica, resulting in differences in glucosinolate synthesis. However, in some varieties, glucosinolate synthesis increased under mixed blue light conditions. These results will help to construct artificial light facilities, which increase functional crops production.
Abstract
In the agricultural industries, LEDs are used as supplementary, as well as main lighting sources in closed cultivation systems. In cultivation using artificial light sources, various light qualities have been tried to supplement fluorescent lamps to promote plant growth and metabolism. Microarray analysis of Brassica rapa seedlings under blue and fluorescent mixed with blue light conditions identified changes in three genes of the glucosinolate pathway. This attracted attention as functional materials highly expressed 3.6-4.6 fold under latter condition. We selected four more genes of the glucosinolate pathway from the Brassica database and tested their expression changes under fluorescent light mixed with red, green, and blue, respectively. Some genes increased expression under red and blue mixed conditions. The Bra026058, Bra015379, and Bra021429; the orthologous genes of CYP79F1, ST5a, and FMOGS-OX1 in Arabidopsis, are highly expressed in Brassica rapa under fluorescent mixed with blue light conditions. Further, Bra029355, Bra034180, Bra024634, and Bra022448; the orthologous genes of MAM1, AOP3, UGT74B1, and BCAT4 in Arabidopsis, are highly expressed in Brassica rapa under fluorescent mixed with red light conditions. The various light conditions had unique effects on the varieties of Brassica, resulting in differences in glucosinolate synthesis. However, in some varieties, glucosinolate synthesis increased under mixed blue light conditions. These results will help to construct artificial light facilities, which increase functional crops production.
참고문헌 (45)
- 안철근, "LED 보광이 파프리카(Capsicum annuum ‘Cupra’) 생육에 미치는 영향", 시설원예‧식물공장, 20(4), pp.253~257 (2011), 한국생물환경조절학회
- Ashburner M, "Gene ontology: tool for the unification of biology. The Gene Ontology Consortium", Nat Genet, 25, pp.25~29 (2000)
- Awad MA, "Effects of light on flavonoid and chlorogenic acid levels in the skin of ‘Jonagold’apples", Sci Hort, 88, pp.289~298 (2001)
- Brew CT, "Indole-3-carbinol inhibits MDA-MB-231 breast cancer cell motility and induces stress fibers and focal adhesion formation by activation of Rho kinase activity", Int J Cancer, 124, pp.2294~2302 (2009)
- Butler WL, "Denaturation of phytochrome", Biochem, 3, pp.851~857 (1964)
- Cha YS, "Investigation of gamma-aminobutyric acid in Chinese cabbage and effects of the cabbage diets on lipid metabolism and liver function of rats administered with ethanol", J Korean Soc Food Sci Nutr, 29, pp.500~505 (2000)
- 조자용, "몇가지LED가아마란서스의종자발아, 새싹의생장및생리활성에미치는영향", 원예과학기술지, 26(2), pp.106~112 (2008), 한국원예학회
- 최만권, "LED광 파장이 상추생육과 비타민 C 및 안토시아닌 함량에 미치는 영향", 시설원예‧식물공장, 23(1), pp.19~25 (2014), (사) 한국생물환경조절학회
- Du Z, "agriGO: a GO analysis toolkit for the agricultural community", Nucleic Acids Res, 38, pp.W64~W70 (2010)
- Ebisawa M, "Supplementary ultraviolet radiation B together with blue light at night increased quercetin content and flavonol synthase gene expression in leaf lettuce (Lactuca sativa L.)", Environ Control Biol, 46, pp.1~11 (2008)
- Faulkner K, "Selective increase of the potential anticarcinogen 4-methylsulphinylbutyl glucosinolate in broccoli", Carcinogenesis, 19, pp.605~609 (1998)
- Halliwell B, "Antioxidants: the basics-what they are and how to evaluate them", Adv Pharmacol, 38, pp.3~20 (1997)
- Hangarter RP, "Gravity, light and plant form", Plant Cell Environ, 20, pp.796~800 (1997)
- 허정욱, "Effects of Supplementary LED Lighting on Growth and Biochemical Parameters in Dieffenbachia amoena ‘Camella’ and Ficus elastica ‘Melany’", 원예과학기술지, 28(1), pp.51~58 (2010), 한국원예학회
- Huseby S, "Diurnal and light regulation of sulphur assimilation and glucosinolate biosynthesis in Arabidopsis", J Exp Bot, 64, pp.1039~1048 (2013)
- 황명근, "SMD형 Y", G, 조명.전기설비학회논문지, pp.2004, 18(4)[DOI]
- Irizarry RA, "Summaries of Affimetrix GeneChip probe level data", Nucleic Acids Res, 31, pp.e15~ (2003)
- Johkan M, "Blue light-emitting diode light irradiation of seedlings improves seedling quality and growth after transplanting in red leaf lettuce", Hortscience, 45, pp.1809~1814 (2010)
- Kim JA, "Comparative mapping, genomic structure, and expression analysis of eight pseudo-response regulator genes in Brassica rapa", Mol Genet Genomics, 287, pp.373~388 (2012)
- 김진아, "LED 광원의 다양한 광질이 배추 유묘의 유전자 발현에 미치는 영향", 원예과학기술지, 31(5), pp.607~616 (2013), 한국원예학회[DOI]
- Kim JK, "Variation of glucosinolates in vegetable crops of Brassica rapa L. ssp. pekinensis", Food Chem, 119, pp.423~428 (2010)
- 김재훈, "식물공장 시스템의 동향과 발전방향", Journal of Plant Biotechnology, 37(4), pp.442~455 (2010), 한국식물생명공학회
- Kim MR, "Bioactivities of sulfur compounds in cruciferous vegetables", 2004 Annual meeting and international symposium on the current prospects of functional and medical food, pp.150~157 (2004), The Korean Society of FSN
- Klein RM, "Reversible effect of green and orange-red radiation on plant cell elongation", Plant Physiol, 63, pp.114~116 (1979)
- Kojima M, "Light stimulation triggered expression of genes coding for vacuolar proton-pump enzymes V-ATPase and V-PPase in buckwheat", Biosci Biotechnol Biochem, 74, pp.1507~1511 (2010)
- Kopsell DA, "Genetic and environmental factors affecting plant lutein", zeaxanthin, 2008, pp.19, 44~46
- 이명렬, "새싹채소 혼합분말이 고지방 식이를 급여한 흰쥐의 지질대사에 미치는 영향", 한국식품영양과학회지, 36(8), pp.965~974 (2007), 한국식품영양과학회
- 이나영, "Effect of Light Emitting Diode Radiation on Antioxidant Activity of Barley Leaf", Applied Biological Chemistry, 53(6), pp.685~690 (2010), 한국응용생명화학회
- Lund EK, "Cell death in the colorectal cancer cell line HT29 in response to glucosinolate metabolites", J Sci Food Agric, 81, pp.959~961 (2001)
- Lund JB, "End-of-day lighting with different red", far-red ratios using light-emitting diodes affects plant growth of Chrysanthemum × morifolium Ramat. ‘Coral Charm’, 2007, pp.24, 1609~1611
- Mun JH, "Effect of light quality on the glucosinolate pathway in Brassica rapa seedling", master, pp.2014
- Nagaharu U, "Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization", Japan J Bot, 7, pp.389~452 (1935)
- Okamoto K, "Development of plant growth apparatus using blue and red LED as artificial light source", Acta Hort, 440, pp.111~116 (1996)
- Osawa T, "A novel antioxidant isolated from young green barley leaves", J Agric Food Chem, 40, pp.1135~1138 (1992)
- Padilla G, "Variation of glucosinolates in vegetable crops of Brassica rapa", Phytochem, 68, pp.4536~4545 (2007)
- Pedras MSC, "The phytoalexins from cauliflower, caulilexins A, B and C:Isolation, structure determination, synthesis and antifungal activity", Phytochem, 67, pp.1503~1509 (2006)
- Redovniković, IR, "Glucosinolates and their potential role in plant", Periodicum Biologorum, 110, pp.297~309 (2008)
- Shiga T, "Effect of light quality on rosmarinic acid content and antioxidant activity of sweet basil, Ocimum basilicum L", Plant Biotechnol, 26, pp.255~259 (2009)
- Smyth GK, "Linear models and empirical bayes methods for assessing differential expression in microarray experiments", Stat Appl Genet Mol Biol, 3, pp.1~25 (2004)
- Urbonavičiūtė A, "Effect of flashing amber light on the nutritional quality of green sprouts", Agronomy Res, 7, pp.761~767 (2009)
- Wang X, "The genome of the mesopolyploid crop species Brassica rapa", Nat Genet, 43, pp.1035~1039 (2011)
- Whitelam GC, "Light and plant development. Annual Plant Reviews, Volume 30" (2007), Blackwell Publishing Ltd
- Workman C, "A new non-linear normalization method for reducing variability in DNA microarray experiments", Genome Biol, 3, pp.1~16 (2002)
- Zang, YX, "Genome-wide identification of glucosinolate synthesis genes in Brassica rapa", FEBS J, 276, pp.3559~3574 (2009)
- Zeeberg BR, "GoMiner: a resource for biological interpretation of genomic and proteomic data", Genome Biol, 4, pp.R28~ (2003)