토양 및 토양수 중 살충제 Clothianidin 및 대사산물 잔류분석법 확립
Determination of Analytical Method for the Insecticide Clothianidin and its Metabolites in Soil and Surface Water
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초록
BACKGROUND: The purpose of this study was toinvestigate the effects of clothianidin on the soil in terms ofclothianidin dissipation and degradation to evaluate itssafety in order to provide an analytical foundation forclothianidin and the 5 metabolites related to it. METHODS AND RESULTS: High-performance liquidchromatography(HPLC) was used to separate clothianidinand its metabolites in this study. In soil, after suppressingdissociation-proned ions with weak alkalic NH4OH andextracting the metabolites with methanol, clothianidin,Methylaminoimidazole(MAI), Methylnitroguanidine(MNG),Thiazolylmethylurea(TZMU) and Thiazolylnitroguanidine(TZNG). Thiazolylmethylguanidine(TMG) were extracted with theaddition of neutral NH4OAC to increasing the intensity ofions. Compounding elements were separated by usingHydrometrix (ChemElutTM) and ion-exchanging Solid-phaseextraction(SPE) Strong cation-exchanger(SCX) and C18were used. The recovery rates of clothianidin and 5metabolites in soil and water ranged from 87.4% to 104.3%. A standard deviation of our analysis for the soil and watersamples were less than 5%. CONCLUSION: Well accepted detection limits forclothianidin and 5 metabolites in soil samples based on adissipation analysis is 0.005 mg/kg and 0.001 mg/L in watersamples. The dissipation concentration of this study wasdecided to be enough to evaluate the dissipation levels ofclothianidin and its metabolites.
Abstract
BACKGROUND: The purpose of this study was toinvestigate the effects of clothianidin on the soil in terms ofclothianidin dissipation and degradation to evaluate itssafety in order to provide an analytical foundation forclothianidin and the 5 metabolites related to it. METHODS AND RESULTS: High-performance liquidchromatography(HPLC) was used to separate clothianidinand its metabolites in this study. In soil, after suppressingdissociation-proned ions with weak alkalic NH4OH andextracting the metabolites with methanol, clothianidin,Methylaminoimidazole(MAI), Methylnitroguanidine(MNG),Thiazolylmethylurea(TZMU) and Thiazolylnitroguanidine(TZNG). Thiazolylmethylguanidine(TMG) were extracted with theaddition of neutral NH4OAC to increasing the intensity ofions. Compounding elements were separated by usingHydrometrix (ChemElutTM) and ion-exchanging Solid-phaseextraction(SPE) Strong cation-exchanger(SCX) and C18were used. The recovery rates of clothianidin and 5metabolites in soil and water ranged from 87.4% to 104.3%. A standard deviation of our analysis for the soil and watersamples were less than 5%. CONCLUSION: Well accepted detection limits forclothianidin and 5 metabolites in soil samples based on adissipation analysis is 0.005 mg/kg and 0.001 mg/L in watersamples. The dissipation concentration of this study wasdecided to be enough to evaluate the dissipation levels ofclothianidin and its metabolites.
참고문헌 (9)
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