ASE 및 SPE 복합정제법을 이용한 친환경농업토양의 다성분잔류농약 분석
Pesticide Multiresidues Analysis of Environmental-friendly Agricultural Soils by the Complex Cleanup Method of Accelerated Solvent Extraction (ASE) and Solid Phase Extraction (SPE)
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
국내의 친환경농산물과 토양에서 자주 검출되는 농약 50성분을 선정하고, 경남지역의 친환경농산물 재배농지의 토양시료 40점을 대상으로, 가속용매추출법(accelerated solvent extraction)과 고상추출법(solid-phase extraction)의 복합처리법으로 정제하고 GC/ECD/NPD, HPLC/UV/FL, GC/MSD 및 HPLC/MSD를 이용한 분석법을 적용하여 잔류농약의 잔류실태를 조사하였다. 적용한 분석법에 의한 농약 회수율은 72~118%의 범위로 평균 95.5%이었고, CV(%)값 평균은 3.0%로 나타났다. 토양 40점 중 잔류농약 검출은 21점에서 20성분이 검출되었으며 검출토양의 농약별 평균 검출량은 endosulfan 0.035, ethoprophos 0.043, chlorpyrifos 0.020, chlorfenapyr 0.023, flufenoxuron 0.047, fenvalerate 0.070, cypermethrin 0.266, lufenuron 0.016, bifenthrin 0.022, fenobucarb/BPMC 0.025, difenoconazole 0.043, fenarimol 0.059, kresoxim-methyl 0.020, tetraconazole 0.026, isoprothiolane 0.039, iprobenfos 0.017, nuarimol 0.014, fluquinconazole 0.156, tebuconazole 0.047 및 oxadiazon이 0.045 mg/kg으로 나타나서 친환경재배농지의 토양환경기준에 잔류농약의 설정이 시급하다고 판단되었다.
Abstract
Fifty substances of pesticide were selected for analysis through the historical investigation of pesticides detected from environmental-friendly agricultural soil, and the environmental-friendly agricultural soils in Gyeongnam area were collected and then were accepted Anve (accelerated solvent extraction) and SPve (solid-phase extraction) as multiresidue extraction and clean up methods suitable to the soils. The pesticide residues were analyzed by using GC/vCD/NPD, HPLC/UV/FL, GC/MSD, or HPLC/MSD. 50 kinds of pesticides for the soils were an average of 95.5% from retrieval ratio of the 72 to 118% range, and the average of 3.0% for CV (%). Among 40 samples of soil, 20 components were detected from pesticide residues of 21 samples, and average amounts detected for these components were 0.035 for endosulfan, 0.043 for ethoprophos, 0.020 for chlorpyrifos, 0.023 for chlorfenapyr, 0.047 for flufenoxuron, 0.070 for fenvalerate, 0.266 for cypermethrin, 0.016 for lufenuron, 0.022 for bifenthrin, 0.025 for fenobucarb/BPMC, 0.043 for difenoconazole, 0.059 for fenarimol, 0.020 for kresoxim-methyl, 0.026 for tetraconazole, 0.039 for isoprothiolane, 0.017 for iprobenfos, 0.014 for nolrimol, 0.156 for fluquinconazole, 0.047 for tebuconazole, and 0.045 mg/kg for oxadiazon. Therefore it is infered that the establishment of pesticide residues limit for environmental-friendly agricultural soil is needed as soon as possible.
참고문헌 (25)
- 안설화, "토양 중 살균제 Pencycuron의 잔류 특성", 농약과학회지, 10(4), pp.296~305 (2006), 한국농약과학회
- ASE 200, "Accelerated Solvent Extractor Operator's Manual, Document No. 031149, Revision 01, Dionex, Sunnyvale, CA, Sect. 3-5", ~ (1995)
- Chen, S., "Simplified pesticide multiresidues analysis in fish by low-temperature cleanup and solid-phase extraction coupled with gas chromatography", mass spectrometry, 2009, pp.113, 1297~1300
- DeLorenzo, M. E., "Toxicity and bioconcentration potential of the agricultural pesticide endosulfan in phytoplankton and zooplankton", Arch. Environ. Contam. Toxicol, 42, pp.173~181 (2002)
- Codex Alimentarius commission, pesticide residues in food-methods of analysis and sampling, "FAO", Joint FAO, 2000, pp.39~47
- FDA, "Pesticide analytical manual. Vol. Ⅰ. Multiresidue Methods. 3rd ed" (1994), FDA
- FDA, "Pesticide analytical manual. Vol.Ⅰ. Section 103: Method Application In Regulatory Analysis. 3rd ed" (1994), FDA
- 현해남, "Groundwater Ubiquity Score를 이용한 제주도 토양 특성별 농약의 지하수 오염가능성 평가", 농약과학회지, 11(3), pp.144~153 (2007), 한국농약과학회
- Jeong, J. Y., "Necessity of practicing the environment-friendly organic agriculture", Food Sci. & Industry, 39, pp.4~8 (2006)
- Karazafiris, E., "New multiresidue method using solid-phase extraction and gas chromatography-microelectron-capture detection for pesticide residues analysis in royal jelly", J. Chrom. A, 1209, pp.17~21 (2008)
- Kaur, I., "Persistence of endosulfan (technical) in water and soil", Environ. Pollut, 79, pp.77~83 (1998)
- Ken, G. D., "Foliar and nontarget deposition from conventional and reduce volume-Pesticide application in greenhouses", J. Agri. Food Chem, 40, pp.2510~2516 (1992)
- 김인선, "친환경농업 해충방제용 제제의 현황과 전망, 그리고 산업화의 중요성", 한국환경농학회지, 28(3), pp.301~309 (2009), 한국환경농학회
- 김기동, "ASE 전처리를 이용한 유기염소계 잔류농약 분석의 효율성 평가 및 채소 시료에의 적용", 한국환경분석학회지, 12(2), pp.144~149 (2009), 한국환경분석학회
- 이주희, "시설재배 참외 중 살균제의 생물학적 반감기", 한국환경농학회지, 28(4), pp.419~426 (2009), 한국환경농학회
- 이종미, "수세 방법에 따른 깻잎의 잔류농약 제거율 연구", 한국식품과학회지, 35(4), pp.586~590 (2003), 한국식품과학회
- Lee, K. B., "In vivo metabolism of endosulfan in carp (Cyprinus carpio L.)", Agri. Chem. & Biotech, 37, pp.194~202 (1994)
- 허장현, "토양 column을 이용한 토성 및 자갈함량별 농약 이동특성", 농약과학회지, 9(4), pp.330~337 (2005), 한국농약과학회
- 박병준, "논토양 환경 중 제초제 molinate의 잔류성과 분해특성", 농약과학회지, 9(1), pp.60~69 (2005), 한국농약과학회
- Pereira, L. A., "Molecularly imprinted solid-phase extraction for the determination of fenitrothion in tomatoes", Anal. & Bioanal. Chem, 393, pp.1063~1072 (2009)
- Philip, W. Lee, "Handbook of residue analytical methods for agrochemicals", 13~17 (2003)
- Ryang, H. S., "Studies on persistence of pesticides in soils and crops under polyethylene film mulching culture", Korean J. Environ. Agric, 7, pp.8~11 (1988)
- 서용찬, "ASE 전처리를 이용한 유기인계 잔류농약 분석의 효율성 평가 및채소 시료에의 적용", 한국환경분석학회지, 11(1), pp.1~5 (2008), 한국환경분석학회
- 신현수, "Zerovalent Iron에 의한 유기염소계 살충제 Endosulfan의 탈염소화", 한국환경농학회지, 28(2), pp.202~208 (2009), 한국환경농학회
- Yoo, S. H., "Perspectives of sustainable agriculture in Korea", Proceedings of International Symposium on Farm Mechanization for Environment Friendly Agriculture, pp.139~163 (1999), Korean Soc. of Agri. Machin