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Two-dimensional electrophoresis analysis of proteins; Bacillus subtilis LTD and its antifungal activity deficient mutant

정혜영|장유신|이영근|장화형|le Thi Dinh
한국환경생물학회 (Korean Society Of Environmental Biology)|2004.12|환경생물 (Korean Journal of Environmental Biology)|제22권 제4호|pp. 487-493

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Abstract

- To investigate the antifungal activity related protein in pesticidal bacteria,a bacterial strain LTD was isolated from soil collected at Gimje in Jeonbuk province,Korea, and identified as Bacillus subtilis LTD based on a API50 CHB kit and 16S rDNAsequencing. It has an antifungal activity against 9 plant pathogenic fungi in a paperdisc assay. The antifungal activity-deficient mutant, B. subtilis mLTD was induced at a5 kGy dose of 60Co gamma radiation. Using the two -dimensional electrophoresis andthe matrix assisted laser desorption ionization time -of-flight mass spectrometry, thecomparison analysis of proteins between the wild and mutant were performed. A majorintracellular serine proteinase IspA (MW: 32.5 kDa), a NAD (P) H dehydrogenase (MW:20.0 kDa), and a stage II sporulation protein AA, SpoIIAA (MW: 14.3 kDa) were detectedonly in the B. subtilis LTD. These results suggested that the functions of these proteinsfound only in the B. subtilis LTD could be closely related to the antifungal activityagainst plant pathogenic fungi.

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