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Genetic Engineering for Detection of Endocrine Disruption using I-18C Gene Expression in Chironomus riparius

곽인실
한국환경생물학회 (Korean Society Of Environmental Biology)|2005.09|환경생물 (Korean Journal of Environmental Biology)|제23권 제3호|pp. 269-274

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Abstract

The 2D/E gel analysis for polypeptide expression reflecting I-18 C gene (early-ecdysterone inducible gene) has conducted the emerged C. riparius adults from larval phaseexposure to tebufenozide acting as an ecdysteroidal molting hormone. Control group, the amountof ORF II of the I-18 C gene was larger than that of ORF I of this gene. After treatments, ORF Iof the I-18 C gene was overexpressed as the polypeptide, whereas ORF II of this gene wasexpressed as the polypeptide and was clearly reduced expression. Accordingly, we consider thattebufenozide exhibited endocrine disruptions related processing of ecdysteroid receptor proteinreflecting ORF II of I-18 C gene. Also, earlier emergence day was related overexpressedpolypeptide reflecting ORF I of I-18 C gene. In this study result, tebufenozide induced changing-ecdysteroneinducible I-18 C gene was different between control group and exposure group.

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