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Expression Changes of Chironomus riparius Proteome reflecting Ecdysterone Inducible Genes and Mitogenome exposure to Bisphenol-A

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Abstract

Development of the fourth-instar larvae of Chironomus ripariushas a sensitive toecdysteroid hormones. The 2D/E gel analysis for polypeptide expression reflecting early-bisphenol A (BPA). In the 2D/E gel 1108 protein spots were identified. The visualized proteinspots allowed extraction of 17 protein spots differed more than 3 fold in BPA treated animals,which was approximately 1.6% of the total protein spots. However, polypeptide expressionreflecting early-ecdysterone inducible gene didn’t change after treatments. In addition, detectionfor the damages or changes in mitogenome level was observed. The conserved cytochrome oxidaseI in DNA level affected exposure to BPA(1g L-1) in this preliminary study.

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