Expression Patterns of the chgH:rfp Transgene in Response to 17α-Ethinylestradiol (EE2) Exposure in Marine Medaka Oryzias dancena
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
The functional utility of a transgenic marine medaka Oryzias dancena strain carrying the red fluorescent protein (RFP) gene driven by an endogenous choriogenin H (chgH) promoter was evaluated for its ability to detect waterborne 17α-ethinylestradiol (EE2), a synthetic estrogen derivative. The chgH:rfp transgenic marine medaka larvae showed an age-dependent tendency in the efficiency of EE2-mediated transgene expression, in which transgenic larvae older than 6 days post-hatching displayed a more effective response in their transgene expression to EE2 than did younger hatchlings. During experimental exposures to high concentrations of EE2 (200 to 1,000 ng/L), the transgenic responses in the hatchlings were broadly dose- and duration-dependent. With exposures using lower doses of EE2 (25, 50 and 100 ng/L), EE2-induced transgenic RFP was also observed in the transgenic larvae, although the lower doses required exposure of longer duration. Under the EE2 exposure and microscope assay conditions used in our study, transgenic marine medaka larvae exhibited a similar degree of EE2-mediated RFP phenotype expression at various salinity levels (0, 15 and 30 ppt).
Abstract
The functional utility of a transgenic marine medaka Oryzias dancena strain carrying the red fluorescent protein (RFP) gene driven by an endogenous choriogenin H (chgH) promoter was evaluated for its ability to detect waterborne 17α-ethinylestradiol (EE2), a synthetic estrogen derivative. The chgH:rfp transgenic marine medaka larvae showed an age-dependent tendency in the efficiency of EE2-mediated transgene expression, in which transgenic larvae older than 6 days post-hatching displayed a more effective response in their transgene expression to EE2 than did younger hatchlings. During experimental exposures to high concentrations of EE2 (200 to 1,000 ng/L), the transgenic responses in the hatchlings were broadly dose- and duration-dependent. With exposures using lower doses of EE2 (25, 50 and 100 ng/L), EE2-induced transgenic RFP was also observed in the transgenic larvae, although the lower doses required exposure of longer duration. Under the EE2 exposure and microscope assay conditions used in our study, transgenic marine medaka larvae exhibited a similar degree of EE2-mediated RFP phenotype expression at various salinity levels (0, 15 and 30 ppt).
참고문헌 (20)
- Aris AZ, "Occurrence of 17α-ethynylestradiol(EE2)in the environment and effect on exposed biota : a review", Environ Intl, 69, pp.104~119 (2014)
- Blewett T, "The effects of temperature and salinity on 17α-ethinylestradiol uptake and its relationship to oxygen consumption in the model euryhaline teleost(Fundulus heteroclitus)", Aquat Toxicol, 127, pp.61~71 (2013)
- Chen H, "Generation of a fluorescent transgenic zebrafish for detection of environmental estrogens", Aquat Toxicol, 96, pp.53~61 (2010)
- Cho YS, "Characterization of estrogen-responsive transgenic marine medaka Oryzias dancena germlines harboring red fluorescent protein gene under the control by endogenous choriogenin H promoter", Transgen Res, 22, pp.501~517 (2013)
- 조영선, "광염성 송사리 Oryzias dancena 성체 및 자어의 염분도 변화에 대한 내성", 한국어류학회지, 22(1), pp.9~16 (2010), 한국어류학회
- Filby AL, "Gene expression profiles revealing the mechanisms of anti-androgen-and estrogeninduced feminization in fish", Aquat Toxicol, 81, pp.219~231 (2007)
- Harding LB, "High-throughput sequencing and pathway analysis reveal alteration of the pituitary transcriptome by 17α-ethynylestradiol(EE2)in female coho salmon Oncorhynchus kisutch", Aquat Toxicol, 142-143, pp.146~163 (2013)
- Hirakawa I, "Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka(Oryzias latipes)exposed to 17α-ethynylestradiol", Chemosphere, 87, pp.668~674 (2012)
- Kim DS, "Comparative analysis of inherited patterns of the transgene in transgenic mud loach Misgurnus mizolepis lines carrying the CAT reporter gene", Fish Sci, 70, pp.201~210 (2004)
- Kurauchi K, "Characteristics of ChgH-GFP transgenic medaka lines, an in vivo estrogenic compound detection system", Mar Pollut Bull, 57, pp.441~444 (2008)
- Lange R, "Effects of the synthetic estrogen 17α-ethinylestradiol on the life-cycle of the fathead minnow(Pimephales promelas)", Environ Toxicol Chem, 20, pp.1216~1227 (2001)
- Lee O, "Biosensor zebrafish provide new insights into potential health effects of environmental estrogens", Environ Health Perspect, 120, pp.990~996 (2012)
- Lee PY, "Environmentally relevant exposure of 17α-ethinylestradiol impairs spawning and reproductive behavior in the brackish medaka Oryzias melastigma", Mar Pollut Bull, 85, pp.338~343 (2014)
- 이상윤, "Gene Structure and Estrogen-Responsive mRNA Expression of a Novel Choriogenin H Isoform from a Marine Medaka Oryzias dancena", Fisheries and Aquatic Sciences, 15(3), pp.221~231 (2012), 한국수산과학회
- Nadzialek S, "Bcl-2 and Caspase 3 mRNA levels in the testes of gudgeon, Gobio gobio, exposed to 17α-ethinylestradiol(EE2)", Aquat Toxicol, 98, pp.304~310 (2010)
- Petersen K, "Transgenic(cyp19a1b-GFP)zebrafish embryos as a tool for assessing combined effects of oestrogenic chemicals", Aquat Toxicol, 138-139, pp.88~97 (2013)
- Shved N, "Environmentally relevant concentrations of 17α-ethinylestradiol(EE2)interfere with the growth hormone(GH)", insulin-like growth factor(IGF)-I system in developing bony fish, 2008, pp.106, 93~102
- Shved N, "Challenge with 17α-ethinylestradiol(EE2)during early development persistently impairs growth, differentiation, and local expression of IGF-I and IGF-II in immune organs of tilapia", Fish Shellfish Immunol, 26, pp.524~530 (2009)
- Teng Q, "Impacts 17α-ethynylestradiol exposure on metabolite profiles of zebrafish(Danio rerio)liver cells", Aquat Toxicol, 130-131, pp.184~191 (2013)
- Zhang X, "Degradation of 17α-ethinylestradiol in aqueous solution by ozonation", J Hazard Mat, 133B, pp.291~298 (2006)