오비트랩 고분해능 질량분석기를 이용한 폐사 조류 중 다성분 잔류 농약 스크리닝 기법
Residual Multi Pesticides Screening of Dead Birds by Orbitrap High Resolution Mass Spectrometry
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참고문헌
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초록
BACKGROUND: The objective of this study was to evaluate screening method of residual multi pesticides in dead birds by Orbitrap high resolution mass spectrometry (HRMS) to identify the cause of death for birds . METHODS AND RESULTS: Extraction and clean-up method of residual pesticides in liver of dead birdswas used QuEChERS (Quick Easy Cheap Effective Rugged and Safe) and method validations was conducted using liquid chromatography and gas chroamtography with triplequadrupole mass spectrometer (LC/MS/MS and GC/MS/ MS) Also, we were evaluated screening method for the determination of residual pesticides in liver of dead birds by LCandGCOrbitrapMass Spectrometry. Results ofmethod validations, Correlation coefficients of thematrixmatched calibration curves were >0.978, and the method detection limits (MDLs) and limits of quantitation (LOQ) were 2.8~ 72.1 ng/g (18.4 ng/g on average) and 9.0~230 ng/g (58.5 ng/g on average). The accuracy ranged from69.1%to 130% (103%on average), and the precision valueswere less than 14.8%(3.8%on average). The screening of residual pesticides in liver of dead birds by LC and GC Orbitrap HRMS was detectedmonocrotophos, carbofuran, carbosulfan, deltametrin, benfuracarb, carbofuran, phosphamidon, prochloraz in investigated samples. CONCLUSION: This results showed that accurate mass were extraction of residual pesticides in dead birds by Orbitrap HRMS. It suggested that this screeningmethod is applicable to the residual pesticide analysis for the cause of death as a main tool.
Abstract
BACKGROUND: The objective of this study was to evaluate screening method of residual multi pesticides in dead birds by Orbitrap high resolution mass spectrometry (HRMS) to identify the cause of death for birds . METHODS AND RESULTS: Extraction and clean-up method of residual pesticides in liver of dead birdswas used QuEChERS (Quick Easy Cheap Effective Rugged and Safe) and method validations was conducted using liquid chromatography and gas chroamtography with triplequadrupole mass spectrometer (LC/MS/MS and GC/MS/ MS) Also, we were evaluated screening method for the determination of residual pesticides in liver of dead birds by LCandGCOrbitrapMass Spectrometry. Results ofmethod validations, Correlation coefficients of thematrixmatched calibration curves were >0.978, and the method detection limits (MDLs) and limits of quantitation (LOQ) were 2.8~ 72.1 ng/g (18.4 ng/g on average) and 9.0~230 ng/g (58.5 ng/g on average). The accuracy ranged from69.1%to 130% (103%on average), and the precision valueswere less than 14.8%(3.8%on average). The screening of residual pesticides in liver of dead birds by LC and GC Orbitrap HRMS was detectedmonocrotophos, carbofuran, carbosulfan, deltametrin, benfuracarb, carbofuran, phosphamidon, prochloraz in investigated samples. CONCLUSION: This results showed that accurate mass were extraction of residual pesticides in dead birds by Orbitrap HRMS. It suggested that this screeningmethod is applicable to the residual pesticide analysis for the cause of death as a main tool.
참고문헌 (20)
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