QuEChERS 법을 이용한 Rat 조직내 Pyraclofos 잔류 분석 및 급성독성 평가
QuEChERS-based determination of tissue residues and acute toxicity of pyraclofos in rat
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Environmental pesticides used for insect control can be transferred from plants to animals even to live-stock animals through food chain. Human beings also can be exposed to pesticides by consuming pol-luted dairy products, including meats, eggs and other milk products. Therefore, the Ministry of Food and Drug Safety (MFDS) established Standard for Pesticide Residue Limits in dairy products. The QuEChERS (quick, easy, cheap, effective, rugged and safe) methods for detecting residual pesticides are relatively well established for fruits and vegetables, however, the methods for meat have not been appropriately studied yet. In the present work, pyraclofos was used as an organophosphate pesticide to examine its tissue residue in experimental animals by QuEChERS methods. For this, pyraclofos (150 mg/kg body weight) was orally administered to male rats once a day for 2 days. After 6, 12, and 24 hr of the treatment, the tissue residues in liver and femoral muscle of the rats were determined using QuEChERS methods followed by HPLC analyses. In preliminary studies, the recovery rates of spiking samples of pyraclofos demonstrated approximately 109∼110% from the tissues. In previous study, pyr-aclofos tissue residues were observed with significantly high levels in livers and muscles at 6 hr of oral treatment. Then, they were almost completely disappeared after 24 hr of the administration, indicating the orally exposed pyraclofos is rapidly absorbed and distributed to body organs, then quickly excreted from the body with a negligible level of tissue residue. The alterations in blood chemistry as well as the histopathology of heart, lung, liver, spleen and kidney have also been investigated in the ex-perimental animals for assessing acute toxic effects of pyraclofos. The obtained blood chemistry indexes (ALT and AST) showed maximum peak values at 12 hr after the oral administration and decreased to the normal levels at 24 hr of the treatment. Histopathologic observation exhibited acute hepatic damages at 24 hr of the treatment. In conclusion, we suggest that QuEChERS method can be adequately opti-mized for the analysis of pyraclofos residues in animal tissues.
Abstract
Environmental pesticides used for insect control can be transferred from plants to animals even to live-stock animals through food chain. Human beings also can be exposed to pesticides by consuming pol-luted dairy products, including meats, eggs and other milk products. Therefore, the Ministry of Food and Drug Safety (MFDS) established Standard for Pesticide Residue Limits in dairy products. The QuEChERS (quick, easy, cheap, effective, rugged and safe) methods for detecting residual pesticides are relatively well established for fruits and vegetables, however, the methods for meat have not been appropriately studied yet. In the present work, pyraclofos was used as an organophosphate pesticide to examine its tissue residue in experimental animals by QuEChERS methods. For this, pyraclofos (150 mg/kg body weight) was orally administered to male rats once a day for 2 days. After 6, 12, and 24 hr of the treatment, the tissue residues in liver and femoral muscle of the rats were determined using QuEChERS methods followed by HPLC analyses. In preliminary studies, the recovery rates of spiking samples of pyraclofos demonstrated approximately 109∼110% from the tissues. In previous study, pyr-aclofos tissue residues were observed with significantly high levels in livers and muscles at 6 hr of oral treatment. Then, they were almost completely disappeared after 24 hr of the administration, indicating the orally exposed pyraclofos is rapidly absorbed and distributed to body organs, then quickly excreted from the body with a negligible level of tissue residue. The alterations in blood chemistry as well as the histopathology of heart, lung, liver, spleen and kidney have also been investigated in the ex-perimental animals for assessing acute toxic effects of pyraclofos. The obtained blood chemistry indexes (ALT and AST) showed maximum peak values at 12 hr after the oral administration and decreased to the normal levels at 24 hr of the treatment. Histopathologic observation exhibited acute hepatic damages at 24 hr of the treatment. In conclusion, we suggest that QuEChERS method can be adequately opti-mized for the analysis of pyraclofos residues in animal tissues.
참고문헌 (14)
- 한국작물보호협회, "농약연보", 한국작물보호협회, ~ (2013)
- "Pesticide Residues in Food. Rome: FAO/WHO Food Standard" (2010)[URL]
- Ecobichon, D.J., "Casarett and Doull’s Toxicology; The Basic Science of Posions", pp.580~592 (1991), Perganmo Press
- Edwards R, "Comparative toxicity of cis-cypermethrin in rainbow trout, frog, mouse, and quail", Toxicol Appl Pharmacol, 84, pp.512~522 (1986)
- Fishel, F. M., "Pesticide use trends in the US: Global comparison", Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida, ~ (2007)
- Kono Y, "Relation of AChE and AliE inhibition of anti-AChE insecticides, TIA-230, fenitrothion and carbaryl to their residual activity in Sodoptera litura larvae(Lepidoptera : Noctuidae)", Applied Entomology and Zoology, 21, pp.363~369 (1986)
- Korea Health Industry Development Institute, "MRLs for pesticides in Foods", 87~88 (2011)
- LeDoux M., "Analytical methods applied to the determination of pesticide residues in foods of animal origin. A review of the past two decades", J chromatogr A, 1218, pp.1021~1036 (2011)
- Lehotay SJ, "Determination of pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulfate: collaborative study", J AOAC Int, l90, pp.485~520 (2007)
- Park JY, "Simultaneous multiresidue analysis of 41 pesticide residues in cooked foodstuff using QuEChERS : Comparison with classical method", Food Chem, 128, pp.241~253 (2011)
- Payà P, "Analysis of pesticide residues using the Quick Easy Cheap Effective Rugged and Safe(QuEChERS)pesticide multiresidue method in combination with gas and liquid chromatography and tandem mass spectrometric detection", Anal Bioanal Chem, 389, pp.1697~1714 (2007)
- Sherman M, "Comparative Toxicity of Four Halogenated Organophosphorus Insecticides to Chicks, Japanese Quail, and Diptera", J econ entomol, 64, pp.814~819 (1971)
- Valverde A, "Analysis of pyraclofos in crops by the Swedish National Food Administration gas chromatographic multiresidue methodology", Analytica Chimica Acta, 338, pp.63~68 (1997)
- Wilkowska A, "Determination of pesticide residues in food matrices using the QuEChERS methodology", Food Chem, 125, pp.803~812 (2011)