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Tin-free 방오제인 Sea-Nine 211에 노출된 북방대합에서 MFO 효소계의 반응

Responses of MFO System in Surf Clam, Pseudocardium sachalinensis, Injected with Sea-Nine 211

전영하|심원준|전중균|이지선
한국환경생물학회 (Korean Society Of Environmental Biology)|2008.06|환경생물 (Korean Journal of Environmental Biology)|제26권 제2호|pp. 102-108

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참고문헌

초록

Abstract

- Many alternative biocidal additives were applied to antifouling paint to replace TBT,and Sea-Nine 211 is one of alternating organic booster compounds used in antifouling paint. Inthis study, extent of Sea-Nine 211 toxicity on marine benthic bivalve is evaluated.Sea-Nine 211 was injected to Surf clam, Pseudocardium sachalinensis, that inhabitate northernpart of Gangwon Province, Korea. Survival rate of the clam and xenobiotics metabolizing enzymeactivities in digestive gland were measured during 4 day-exposure period. The results were com-pared with those of TBT exposed clam. There were no mortality of clam in the solvent (DMSO)control group and the three Sea-Nine 211 exposure groups(5, 25, 50 mg kg-1 body weight), whilethe clam exposed to 1, 2 and 5 mg kg-1 TBT chloride (TBTC) demonstrated 70, 30 and 0% sur-vival rate, respectively. The Sea-Nine 211 exposure group showed a tendency of cytochrome P450(CYP) induction according to the exposure duration, on the other hand, CYP content was decr-eased in the TBT exposure group. NADPH cytochrome P450 reductase activity slightly increaseaccording to the exposure duration in the Sea-Nine 211 exposure group, while TBTC inhibit itsactivity as CYP content. Moreover, there was no significant change of NADH cytochrome b5 re-ductate activity in the clam epxosed to Sea-Nine 211. In the TBTC exposure group, its activity in-creased in early exposure period and then significantly decreased the rest of exposure period.All the results indicate that Sea-Nine 211 demonstrated a tendency to induce CYP level, whileTBTC inhibits the CYP level, NADPH cytochrome P450 reductase and NADH cytochrome b5reductase activities.

참고문헌 (40)

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