Tin-free 방오제인 Sea-Nine 211을 주사한 북방대합에서 alkoxyresorufin 탈알킬화효소와 글루타치온 포합효소 활성의 변화
Responses of Alkoxyresorufin Dealkylases and Glutathione S-transferase Activities of Surf Clam, Pseudocardium sachalinensis, Injected with Sea-Nine 211 Antifoulant
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Abstract
- To evaluate the extent of Sea-Nine 211(4,5-dichloro-2-n-octyl-3(2H) isothiazolone), oneof the alternating organic booster compound used in antifouling paint to replace TBT, on marinebenthic bivalve, we injected Sea-Nine 211 to clam, Pseudocardium sachalinensis, and then deter-mined some xenobiotics metabolizing enzyme activities, especially EROD (ethoxyresorufin dee-thylase) and MROD (methoxyresorufin demethylase), in digestive gland during 4 day-exposureperiod. Moreover, the results were compared with those of TBT exposed clam.CYP1A1 dependant EROD activity in both the Sea-Nine 211 and the TBTC exposure groupsshowed no significant differences compared to those of the solvent control group. CYP1A2 dep-endant MROD activity in Sea-Nine 211 exposure group was significantly induced, but no sig-nificant difference was obtained in the TBTC exposure group.These results indicate that Sea-Nine 211 demonstrated a tendency to induce MROD activity,while TBTC inhibits the activities of this enzyme.
참고문헌 (29)
- 이지선, "Tin-free 방오제인 Sea-Nine 211에 노출된 북방대합에서 MFO 효소계의 반응", 환경생물, 26(2), pp.102~108 (2008), 한국환경생물학회
- 전중균, "유기주석화합물이 명주조개 (Coelomactra antiquata) 약물대사효소계에 미치는 영향", 한국수산학회지, 35, pp.185~ (2002)
- 전중균, "유기주석화합물이 해산 어류의 간장 MFO 효소계에 미치는 영향", 환경생물, 21(1), pp.18~25 (2003), 한국환경생물학회
- Alzieu C., "Environmental problems caused by TBT in France: Assessment, regulations, prospects", Mar. Environ. Res, 32, pp.7~17 (1991)
- Burke MD, "Ethoxyresorufin: direct fluorimetric assay of a microsomal O-dealkylation which is preferentially inducible by 3-methylcholanthrene", Drug Metab. Dispos, 2, pp.583~588 (1974)
- Callow ME, "Degradation of antifouling biocides", Biofouling, 10, pp.239~249 (1996)
- Davies IM, "Effects of tributyltin compounds from antifoulants on Pacific oysters(Crassostrea gigas)in Scottish sea lochs", Aquaculture, 74, pp.319~330 (1988)
- Fent K, "Inhibition of hepatic microsomal monooxygenase system by organotins in vitro in freshwater fish", Aquat. Toxicol, 28, pp.107~126 (1994)
- Fent K, "Effects of tributyltin chloride in vitro on the hepatic microsomal monooxygenase system in the fish Stenotomus chrysops", Aquat. Toxicol, 20, pp.159~168 (1991)
- Fent K, "Effects of tributyltin chloride in vitro on hepatic microsomal cytochrome P450 and associated enzyme activities in the marine fish Stenotomus chrysops", 7th International Symposium on Responses of Marine Organisms to Pollutants, pp.210~211 (1993), Elsevier
- Fent K, "Effects of tributyltin in vivo on hepatic cytochrome P450 forms in marine fish", Aquat. Toxicol, 24, pp.219~240 (1993)
- Jacobson A, "Fate of an antifoulant in an aquatic environment, In Pesticides in the Urban Environments, Fate and Significance", pp.127~138 (1993), American Chemical Society
- Jacobson A, "Sea-Nine antifoulant: an environmentally acceptable alternative to organotin antifoulants", The Science of The Total Environ, 258, pp.103~110 (2000)
- Kobayashi N., "Marine pollution bioassay by using sea urchin eggs in the Tanabe Bay", Mar. Pollut. Bull, 23, pp.709~713 (1991)
- Kobayashi N, "Effects of new antifouling compounds on the development of sea urchin", Mar. Pollut. Bull, 44, pp.748~751 (2002)
- Lemaire P, "Stimulation of oxyradical production of hepatic microsomes of flounder (Platichthys flesus) and perch (Perca fluviatilis) by model and pollutant xenobiotics", Arch. Environ. Contam. Toxicol, 26, pp.191~200 (1994)
- Lowry OH, "Protein measurement with the Folin phenol reagent", J. Biol. Chem, 193, pp.265~275 (1951)
- Marin MG, "Embryotoxicity of butyltin compounds to the sea urchin Paracentrotus lividus", Mar. Environ. Res, 50, pp.231~235 (2000)
- Morcillo Y, "Interaction of tributyl- and triphenyltin with the microsomal monooxygenase system of molluscs and fish from the western Mediterranean", Aquat. Toxicol, 38, pp.35~46 (1997)
- Morcillo Y, "Effects of tributyltin on the cytochrome P450 monooxygenase system and sex steroid metabolism in the clam Ruditapes decussata", Mar. Environ. Res, 46, pp.583~586 (1998)
- Pesticides Safety Directorate, "Antifouling Products" (1998), The Stationary Office
- Peters LD, "Immunochemical investigations of cytochrome P450 forms", epitopes (CYP1A, 2B, 2E, 3A and 4A) in digestive gland of Mytilus sp, 1998, pp.121C, 361~369
- Shade WD, "Ecological risk assessment of a novel marine antifoulant", American Society for Testing and Materials (ASTM) Special Technical Publication (STP) 1216, 381~407 (1993)
- Solé M, "Effects of tributyltin on the MFO system of the clam Ruditapes decussata: a laboratory and field approach", Comp. Biochem. Physiol, 125C, pp.93~101 (2000)
- International Programme on Chemical Safety Environmental Health Criteria 116 Tributyltin Compounds, Geneva, "World Health Organization", ~ (1990)
- Willemsen PR, "Repetitive testing of TBTO, Sea-Nine 211 and farnesol using Balanus amphitrite (Darwin) cypris larvae: Variability in larval sensitivity", Biofouling, 12, pp.133~147 (1998)
- Willingham GL, "Efficacy and environmental fate of a new isothiazolone antifoulant", Proceedings of the 3rd Asia-Pacific Conference of the Paint Research Association, pp.14.1~14.13 (1993)
- Willingham GL, "Designing an environmentally safe marine antifoulant, In Designing Safer Chemicals", pp.224~233 (1996), American Chemical Society
- Wootton AN, "Evidence for the existence of cytochrome P450 gene families (CYP1A, 3A, 4A, 11A) and modulation of gene expression (CYP1A) in the mussel, Mytilus edulis sp", Mar. Environ. Res, 39, pp.21~26 (1995)
