Deltamethrin에 노출된 흰쥐의 뇨 중 3-PBA 검출 및 노출상관성
The Correlation Between Deltamethrin Exposure and Urinary 3-PBA Concentrations in Rats
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BACKGROUND: Pyrethroids (PYRs) are a widely used insecticide in agriculture and household area. In mammals, PYRs such as deltamethrin is metabolized to 3-phenoxybenzoic acid (3-PBA) in liver that is mainly excreted in urine. This study is designed to single exposure of deltamethrin to rats in a dose-dependent manner and identify the correlation between deltamethrin exposure and its metabolite (3-PBA) in urine. METHODSANDRESULTS:Exposure levels of deltamethrin were control (0 mg/kg bw), low (0.0705 mg/kg bw), medium(0.705mg/kg bw) and high (7.05mg/kg bw) dose. Lowconcentrationwas derived by ussingKorea predictive operator exposure model (KoPOEM). Dermal exposure persisted for 6 h, and urine specimenswere collected for 24 h. The urinematrixwas removed after a series of procedures and 3-PBA was analyzed by gas chromatography/mass spectrometry. CONCLUSION: There was a strong correlation (R2=0.83) between the amount of oral exposure to delta me thrin and urinary levelof3-PBAexcreted. In dermal exposure groups of deltamethrin except high-dose, also there was a good correlation between urinary 3-PBA and deltamethrin exposure, but not stronger than in oral deltamethrin exposure groups. Based on these results, therefore, the amount of 3-PBAin urine can be used as a goodmonitoring indicator that reflexing the exposure level of deltamethrin to human body.
Abstract
BACKGROUND: Pyrethroids (PYRs) are a widely used insecticide in agriculture and household area. In mammals, PYRs such as deltamethrin is metabolized to 3-phenoxybenzoic acid (3-PBA) in liver that is mainly excreted in urine. This study is designed to single exposure of deltamethrin to rats in a dose-dependent manner and identify the correlation between deltamethrin exposure and its metabolite (3-PBA) in urine. METHODSANDRESULTS:Exposure levels of deltamethrin were control (0 mg/kg bw), low (0.0705 mg/kg bw), medium(0.705mg/kg bw) and high (7.05mg/kg bw) dose. Lowconcentrationwas derived by ussingKorea predictive operator exposure model (KoPOEM). Dermal exposure persisted for 6 h, and urine specimenswere collected for 24 h. The urinematrixwas removed after a series of procedures and 3-PBA was analyzed by gas chromatography/mass spectrometry. CONCLUSION: There was a strong correlation (R2=0.83) between the amount of oral exposure to delta me thrin and urinary levelof3-PBAexcreted. In dermal exposure groups of deltamethrin except high-dose, also there was a good correlation between urinary 3-PBA and deltamethrin exposure, but not stronger than in oral deltamethrin exposure groups. Based on these results, therefore, the amount of 3-PBAin urine can be used as a goodmonitoring indicator that reflexing the exposure level of deltamethrin to human body.
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