소망막내피세포에서 금 나노입자의 최종당화산물에 의한 세포 이동 및 침윤성 억제 효과
Gold Nanoparticles Inhibit AGEs Induced Migration and Invasion in Bovine Retinal Endothelial Cells
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
본 연구는 BRECs에서 AGEs로 유도된 세포의 이동및 침윤에 있어서 AuNP의 역할에 관한 연구이다. 소 망막으로부터 내피세포를 분리하고, 세포 생존율은 MTT assay로 확인하였다. Wound migration assay는 BRECs의이동력을 확인하기 위해 수행하였다. Tube formation은on-gel system을 통해 확인하였다. AuNP의 apoptosis 유도는 caspase-3 assay를 통해 확인하였다. AGE-BSA은세포증식 및 이동에 있어서 증가함을 보여주었다. 또한AuNP는 AGE-BSA 존재 유무에 상관없이 세포의 증식,이동, 신생혈관 형성을 억제하였고, caspase-3을 통해apoptosis를 유도하였다. 이러한 결과, AuNP는 AGE로유도된 신생혈관 형성 및 세포의 이동성을 억제하는 약재제로서, 당뇨성 합병증에 있어서 잠재적으로 중요한분자가 될 것이다.
Abstract
This study aimed the role of gold nanoparticles (AuNP) in advanced glycation end-products (AGEs) induced migration and invasion in bovine retinal endothelial cells (BRECs). BRECs were isolated from the retina. Cell viability was confirmed by the MTT assay. In vitro wound migration assay was performed to investigate the migration of BRECs. In vitro tube formation was measured by on-gel system. Apoptosis induced by AuNP was confirmed by caspase-3 assay. AGEbovine serum albumin (BSA) demonstrated increase of cell migration and proliferation in BRECs. In addition, AuNP regardless of the existence of AGE-BSA suppressed proliferation, migration,and angiogenesis. AuNP suppressed AGE-BSA induced migration and invasion, and induced apoptosis through caspase-3. As a results, AuNP have a potential anti-angiogenic effect for AGE-induced angiogenesis in vitro and offer possibility for the treatment of diabetic retinopathy.
참고문헌 (23)
- Chen L, "PEDF induces apoptosis in human endothelial cells by activating p38MAP kinase dependent cleavage of multiple caspases", Biochemical and Biophysical Research Communications, 348, pp.1288~1295 (2006)
- Cho KH, "The study of antimicrobial activity and preservative effects of nanosilver ingredient", Electrochimica. Acta, 51, pp.956~960 (2005)
- Dona M, "Hyperforin inhibits cancer invasion and metastasis", Cancer Res, 64, pp.6225~6232 (2004)
- Frye CA, "Isolation and culture of rat microvascular endothelial cells", In vitro Cell Developmental Biology Animal, 38, pp.208~212 (2002)
- Goldin A, "Advanced glycation end products: sparking the development of diabetic vascular injury", Circulation, 114, pp.597~605 (2006)
- Grabar KC, "Preparation and characterization of Au colloid monolayers", Anal. Chem, 67, pp.735~743 (1995)
- Hutching H, "Pigment epithelium-derived factor exerts opposite effects on endothelial cells of different phenotypes", Biochemical and Biophysical Research Communications, 294, pp.764~769 (2002)
- Li ZP, "Selective determination of cysteine by resonance light scattering technique based on self-assembly of gold nanoparticles", Anal. Biochem, 351, pp.18~34 (2006)
- Mosmann T, "Rapid colorimetric assay for cellular growth and survival: Application of proliferation and cytotoxicity assays", J. Immunol. Methods, 65, pp.55~63 (1983)
- Moon A, "H-ras but not N-ras, induces an invasive phenotype in human breast epithelial cells: a role for MMp-2 in the H-ras-induced invasive phenotype", Int. J. Cancer, 85, pp.176~181 (2000)
- Moore TCB, "The role of advanced glycation end products in retinal microvascular leukostatis", Investgative Ophthalmology and Visual Science, 44, pp.4457~4464 (2003)
- Nicholson DW, "Identification and inhibition of the ICE", CED-3 protease necessary for mammalian apoptosis, 1995, pp.376, 37~43
- Resham B, "Biological properties of naked metal nanoparticles", Advanced Drug Delivery Reviews, pp.1289~1306 (2008)
- Ren JG, "How PEDF prevents angiogenesis: a hypothesized pathway", Medical Hypothesis, 64, pp.74~78 (2005)
- Sakata N, "Effects of advanced glycation end products on the proliferation and fibronectin production of smooth muscle cells", Journal of Atherosclerosis and Thrombosis, W7, pp.169~176 (2000)
- Spranger J, "Loss of anti-angiogenic pigment epithelium derived factor in patients with angiogenic eye disease", Diabetes, 50, pp.2641~2645 (2002)
- Stitt AW, "Advanced glycation end products induce bloodretinal barrier dysfunction in normoglycemic rats", Molecular Cell Biology Research Communications, 3, pp.380~388 (2000)
- Stitt AW, "Impaired retinal angiogenesis in diabetes: role of advanced glycation end products and galectin-3", Diabetes, 53, pp.785~794 (2005)
- Su X, "Isolation and characterization of murine retinal endothelial cells", Molecular Vision, 9, pp.174~178 (2003)
- Tanaka N, "The receptor for advanced glycation end products is induced by glycation products themselves and tumor necrosis factor-α through nuclear factor-κB and by 17β-estradiol through sp-1 in human vascular endothelial cells", Journal of Biology Chemistry, 275, pp.25781~25790 (2000)
- Volpert OV, "Inducer-stimulated Fas targets activated endothelium for destruction by anti-angiogenic thrombospondin-1 and pigment epithelium-derived factor", Nature Medicine, 8, pp.349~357 (2002)
- Wautier JL, "Advanced glycation endproducts, their receptors and diabetic angiopathy", Diabetes Metabolism, 21, pp.535~542 (2001)
- Yamagishi S, "Role of advanced glycation end products (AGEs) and their receptor (RAGE) in the pathogenesis of diabetic microangiopathy", International Journal of Clicical and Pharmacological Research, 23, pp.129~134 (2003)