Relationship between White Spot Symptom and Physiological Status of Two Penaeid Shrimps
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Shrimps infected with WSSV (White Spot Syndrome Virus) generally exhibit white spots in their inner space of carapaces as an acute clinical sign. In an effort to identify the correlation between this acute clinical sign and the condition, the index factors (RNA/DNA concentration and ratio, trypsin activity) were analyzed. A total 580 farmed Fenneropenaeus chinensis and 130 Lithopenaeus vannamei were collected from western and southern fifteen outdoor ponds in Korea. The status of the white spot pathology was divided into four stages (stage 0, stage I, stage II, and stage III), in accordance with the clinical signs as to the size and area of white spots. A significant decrease in RNA concentration and RNA/DNA ratio for multi-infected fleshy prawn (WSSV and vibrio sp.) occurred during the stage III (the whole carapace is covered with a white spot). In particular, RNA/DNA ratio was significantly lower as 1.47±0.04 than other groups. A similar trend was also found in the single infection (WSSV), but the decrease was less than the multi-infection. In the species comparison, both species were vulnerable to the multi-infection, but L. vannamei was more sensitive than F. chinensis (ANOVA, p<0.05): A significant decrease in RNA concentration and RNA/DNA ratio was first found in stage II for the former species, while it was found in stage III for the latter species. Trypsin activity was also showed a similar tendency with nucleic acid variation. Multi-infected shrimp showed drastically decrease of trypsin activity. According to the results, clinical signs of the white spot under carapace have an only physiological effect on shrimp if they covered entirely with white spots.
Abstract
Shrimps infected with WSSV (White Spot Syndrome Virus) generally exhibit white spots in their inner space of carapaces as an acute clinical sign. In an effort to identify the correlation between this acute clinical sign and the condition, the index factors (RNA/DNA concentration and ratio, trypsin activity) were analyzed. A total 580 farmed Fenneropenaeus chinensis and 130 Lithopenaeus vannamei were collected from western and southern fifteen outdoor ponds in Korea. The status of the white spot pathology was divided into four stages (stage 0, stage I, stage II, and stage III), in accordance with the clinical signs as to the size and area of white spots. A significant decrease in RNA concentration and RNA/DNA ratio for multi-infected fleshy prawn (WSSV and vibrio sp.) occurred during the stage III (the whole carapace is covered with a white spot). In particular, RNA/DNA ratio was significantly lower as 1.47±0.04 than other groups. A similar trend was also found in the single infection (WSSV), but the decrease was less than the multi-infection. In the species comparison, both species were vulnerable to the multi-infection, but L. vannamei was more sensitive than F. chinensis (ANOVA, p<0.05): A significant decrease in RNA concentration and RNA/DNA ratio was first found in stage II for the former species, while it was found in stage III for the latter species. Trypsin activity was also showed a similar tendency with nucleic acid variation. Multi-infected shrimp showed drastically decrease of trypsin activity. According to the results, clinical signs of the white spot under carapace have an only physiological effect on shrimp if they covered entirely with white spots.
참고문헌 (31)
- Briggs M, "Introductions and movement of Penaeus vannamei and Penaeu stylirostris in Asia and the Pacific", FAO Regional Office for Asia and the Pacific, 1~92 (2004)
- Buckley L., "RNA : DNA ratio and index of larval fish growth in the sea", Mar. Biol, 80, pp.291~298 (1984)
- Bulow JF, "The Age and Growth of Fish", pp.45~64 (1987), The Iowa State University Press
- Chang PS, "Identification of white spot syndrome associated baculovirus (WSBV) target organs in shrimp, Penaeus monodon, by in situ hybridization", Dis. Aquat. Org, 27, pp.131~139 (1996)
- Chen LL, "Natural and experimental infection of white spot syndrome virus (WSSV) in benthic larvae of mud crab Scylla serrata", Dis. Aquat. Org, 40, pp.157~161 (2000)
- Chìcharo MA, "Estimation of starvation and dial variation of the RNA", DNA ratios of field caught Sardina plichardus larvae off north of Spain, 1998, pp.154, 273~283
- Clark A, "Growth rate and nucleic acid ratio in cultured cuttlefish, Sepia officinalis (Mollusca: Cephalopoda)", J. Exp. Mar. Biol. Ecol, 133, pp.229~240 (1989)
- Clemmesen C., "Improvements in the fluorimetric determination of the RNA and DNA content of individual marine fish larvae", Mar. Ecol. Prog. Ser, 100, pp.177~183 (1993)
- Dortch Q, "RNA", DNA ratios and DNA concentrations as indicators of growth rate and biomass in planktonic organism, 1983, pp.13, 61~71
- Durand S, "Ultrastructure and morphogenesis of white spot syndrome bacalovirus (WSSV)", Dis. Aquat. Org, 29, pp.205~211 (1997)
- Galgani ML, "Identification of digestive proteinases of Penaeus kerathurus (Forskal): a comparison with Penaeus japonicus", Comp. Biochem. Physiol, 788, pp.355~361 (1984)
- Grémare A, "Comparison of several spectrofluorometric methods for measuring RNA and DNA concentrations in the deposit-feeding bivalve Abra ovata", Comp. Biochem. Physiol, 107B, pp.297~308 (1994)
- Ikeda T, "RNA", DNA ratios of calanoid copepods from the epipelagic through abyssopelagic zones of the North Pacific Ocean, 2007, pp.1, 99~108
- 장인권, "축제식 양식장에서의 대하, 흰다리새우와 황복의 복합양식", 한국양식학회지, 20(4), pp.278~288 (2007), 한국양식학회
- 김수경, "Comparison of Nucleic Acid Levels, Ratio and Ecophysiological Aspects among three Populations of the Fleshy prawn Fenneropenaeus chinensis in Korea", Fisheries and Aquatic Sciences, 9(1), pp.7~13 (2006), 한국수산과학회
- Lavilla-Pitogo CRM, "Occurrence of luminous bacterial disease of Penaeus monodon larvae in the Philippines", Aquaculture, 91, pp.1~13 (1990)
- Lemos D, "Ontogenetic variation in digestive proteinase activity, RNA and DNA content of larval and postlarval white shrimp Litopenaeus schmitti", Aquaculture, 214, pp.363~380 (2002)
- Leu JH, "Comparative analysis of differentially expressed genes in normal and white spot syndrome virus infected Penaeus monodon", BMC Genomics, 8, pp.120~ (2007)
- Lightner DV, "CRC Handbook of Mariculture", pp.289~321 (1983), CRC Press
- Lightner DV, "Experimental infection of white spot syndrome of western Hemisphere penaeid shrimp with Asian White spot syndrome virus and Asian yellow head virus", J. Aquat. Anim. Health, 10, pp.271~281 (1998)
- Leightner DV, "Shrimp diseases and current diagnostic methods", Aquaculture, 164, pp.201~220 (1998)
- MIFAFF, "Food, agriculture, forestry and fisheries statistical yearbook", pp.309~ (2012)
- Muhila-Almazán A, "Effects of dietary protein on the activity and mRNA level of trypsin in the midgut gland of the white shrimp Penaeus vannamei", Com. Biochem. Physiol. B. Biochem. Mol. Biol, 135, pp.373~383 (2003)
- Moss SM, "Growth rates, nucleic acid concentrations, and RNA", DNA ratios of juvenile white shrimp, Penaeus vannamei Boone, fed different algal diets, 1994, pp.182, 193~204
- Nash G, "Diseases in Asian Aquaculture", pp.143~155 (1992), Asian Fisheries Society
- Rosa R, "RNA, DNA and protein concentrations and amino acid profiles of deep-sea decapod Aristeus antennatus; An indication for seasonal variations of nutrition and growth", Aquat. Living Resour, 17, pp.25~30 (2004)
- Thorpe JE, "Bimodality of growth and smolting in Atlantic salmon, Salmo salar L", Aquaculture, 28, pp.123~132 (1982)
- Ueberschär B., "Physiological and Biochemical Aspects of Fish Development", pp.233~239 (1993), University of Bergen
- Wagner M, "RNA : DNA ratios as indicators of nutritional condition in the copepod Calanus finmarchicus Mar", Ecol. Prog. Ser, 162, pp.173~181 (1998)
- Witteveldt J, "Protection of Penaeus monodon against white spot syndrome virus using a WSSV subunit vaccine", Fish & Shellfish Immunol, 16, pp.571~579 (2004)
- Yoganandhan K, "Biochemical, physiological and hematological changes in white spot syndrome virus-infected shrimp, Penaeus indicus", Aquaculture, 221, pp.1~11 (2003)