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국내 잎들깨에서 발생한 잠두위조바이러스2의 특성 구명

Characterization of broad bean wilt virus 2 isolated from Perilla frutescens in Korea

김미경|최현용|이봉춘|김현선|변희성|최유지|서장균|최홍수|곽해련
한국환경생물학회 (Korean Society Of Environmental Biology)|2023.03|환경생물 (Korean Journal of Environmental Biology)|제41권 제1호|pp. 1-13

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참고문헌

키워드

broad bean wilt virus 2pathogenicitygenome sequencesymptomatic variation

초록

잠두위조바이러스2 (BBWV2)는 경제적으로 중요한 원예, 관상작물에 심각한 피해를 주는 바이러스 중의 하나다. BBWV2의 넓은 기주 범위, 매개충 방제의 어려움 등 효과적인 치료제가 없기 때문에, 병을 예방하거나 저항성 품종의 개발 등을 위해서는 BBWV2의 새로운 분리주들의 특성 조사와 연구가 필요하다. 본 연구에서는 2019년 금산지역의 잎들깨 재배농가에서 분리된 BBWV2 분리주 (BBWV2-GS-PF)의 유전학적·생물학적 특성을 구명하고 기존에 보고된 분리주들과의 특성을 비교하였다. 순수분리된 BBWV2-GS-PF는 기존 분리주와 달리 들깨에서 심한 모자이크 증상과 고추에서 원형 반점 증상을 보였다. BBWV2-GS-PF의 유전자 계통분석 결과, 기존에 알려진 2개의 그룹과 약 76~80%의 비교적 낮은 유전자 상동성을 보이며 계통이 분화된 것을 확인할 수 있었다.

Abstract

Broad bean wilt virus 2 (BBWV2) is a species in the genus Fabavirus and family Secoviridae, which is transmitted by aphids and has a wide host range. The BBWV2 genome is composed of two single-stranded, positive-sense RNAs, RNA-1 and RNA-2. The representative symptoms of BBWV2 are mosaic, mottle, vein clearing, wilt, and stunting on leaves, and these symptoms cause economic damage to various crops. In 2019, Perilla fructescens leaves with mosaic and yellowing symptoms were found in Geumsan, South Korea. Reverse-transcription polymerase chain reaction (RTPCR) was performed with specific primers for 10 reported viruses, including BBWV2, to identify the causal virus, and the results were positive for BBWV2. To characterize a BBWV2 isolate (BBWV2-GS-PF) from symptomatic P. fructescens, genetic analysis and pathogenicity tests were performed. The complete genomic sequences of RNA-1 and RNA-2 of BBWV2-GS-PF were phylogenetically distant to the previously reported BBWV2 isolates, with relatively low nucleotide sequence similarities of 76-80%. In the pathogenicity test, unlike most BBWV2 isolates with mild mosaic or mosaic symptoms in peppers, the BBWV2-GS-PF isolate showed typical ring spot symptoms. Considering these results, the BBWV2-GS-PF isolate from P. fructescens could be classified as a new strain of BBWV2.

참고문헌 (34)

  1. Asada M, "Glycoprotein derived from the hot water extract of mint plant, Perilla frutescens Britton", J. Agric. Food Chem, 47, pp.468~472 (1999)[DOI]
  2. Benner CP, "Identification and incidence of pepper viruses in Northeastern Georgia", Plant Dis, 69, pp.999~1001 (1985)
  3. Brunt AA, "Viruses of Plants" (1996), CAB International
  4. Chang MU, "Studies on viruses isolated from Lilium spp. in Korea", Plant Pathol. J, 3, pp.223~235 (1987)
  5. Cho IS, "First report of cucumber green mottle mosaic virus infecting Perilla frutescens in Korea and the world", J. Plant Pathol, 103, pp.347~ (2021)[DOI]
  6. 조점덕, "채소류의 토마토 반점 위조 바이러스 발생과 병징(I)", 식물병 연구, 11(2), pp.213~216 (2005), 한국식물병리학회
  7. Choi HS, "Broad bean wilt fabaviruses and their specific ultrastructures", Kor. J. Electron Microscopy, 31, pp.215~222 (2001)
  8. Dhyani A, "A review on nutritional value, functional properties and pharmacological application of Perilla (Perilla frutescens L.)", Biomed Pharmacol. J, 12, pp.649~660 (2019)[DOI]
  9. Ferrer RM, "Genetic variation and evolutionary analysis of broad bean wilt virus 2", Arch. Virol, 156, pp.1445~1450 (2011)[DOI]
  10. Hahm YI, "Serveys on disease occurrence in major horticultural crops in Kangwon Alpine areas", Plant Pathol. J, 14, pp.668~675 (1998)
  11. Kawahata T, "A novel substance purified from Perilla frutescens Britton inhibits an early stage of HIV-1 replication without blocking viral adsorption", Antivir. Chem. Chemother, 13, pp.283~288 (2002)
  12. Kim MH, "Genetic plasticity in RNA2 is associated with pathogenic diversification of broad bean wilt virus 2", Virus Res, 304, pp.198533~ (2021)[DOI]
  13. 고형래, "국내 들깨 재배지 식물기생선충 감염현황", 환경생물, 39(2), pp.147~155 (2021), 한국환경생물학회
  14. KOSIS, "Crop production survey index. Korea Statistical Information Service", Statistics Korea, ~ (2020)
  15. Kubota K, "Perilla mosaic virus is a highly divergent Emaravirus transmitted by Shevtchenkella sp. (Acari: Eriophyidae)", Phytopathology, 110, pp.1352~1361 (2020)[DOI]
  16. Kumar S, "MEGA X:molecular evolutionary genetics analysis across computing platforms", Mol. Biol. Evol, 35, pp.1547~1549 (2018)[DOI]
  17. 곽해련, "Genetic Compositions of Broad bean wilt virus 2 Infecting Red Pepper in Korea", The Plant Pathology Journal, 29(3), pp.274~284 (2013), 한국식물병리학회[DOI]
  18. 곽해련, "Molecular Characterization and Variation of the Broad bean wilt virus 2 Isolates Based on Analyses of Complete Genome Sequences", The Plant Pathology Journal, 29(4), pp.397~409 (2013), 한국식물병리학회[DOI]
  19. Kwak HR, "A determinant of disease symptom severity is located in RNA2 of broad bean wilt virus 2", Virus Res, 211, pp.25~28 (2016)[DOI]
  20. Kwak HR, "First report of broad bean wilt virus 2 in Gynura procumbens in Korea", Plant Dis, 101, pp.514~ (2017)[DOI]
  21. 곽해련, "국내 분리 토마토반점위조바이러스의 저항성 판별을 위한 생물검정법 개발", 환경생물, 39(3), pp.319~328 (2021), 한국환경생물학회
  22. Lee BH, "Reference review for the scientific researches on perilla", RDA J. Ind. Crop. Sci, 40, pp.80~112 (1998)
  23. Lee SH, "Investigations on the virus diseases in spinach (Spinacia oleracea L.) II. Identification of broad bean wilt virus occuring spinach", Kor. J. Plant Port, 18, pp.11~14 (1979)
  24. Lee U, "Broad bean wilt virus causes necrotic symptoms and generates defective RNAs in Capsicum annuum", Phytopathol, 90, pp.1390~1395 (2000)[DOI]
  25. Nakamura S, "Complete nucleotide sequence and genome organization of broad bean wilt virus 2", Phytopathol. Soc. Jpn, 64, pp.565~568 (1998)
  26. Park IS, "The viruses in Gladiolus hybridus cultivated in Korea 2. Broad bean wilt virus, Cucumber mosaic virus and Tobacco rattle virus", Plant Pathol. J, 14, pp.83~91 (1998)
  27. Park SH, "First report of turnip mosaic virus infecting Perilla frutescens in Korea", J. Plant Pathol, 102, pp.969~ (2020)[DOI]
  28. Roh SH, "Three viruses isolated from Gentiana spp. in Korea", Plant Pathol. J, 14, pp.425~432 (1998)
  29. Seo JK, "Movement protein of broad bean wilt virus 2 serves as a determinant of symptom severity in pepper", Virus Res, 242, pp.141~145 (2017)[DOI]
  30. Seo WH, "Characteristic aroma-active compounds of Korean perilla (Perilla frutescens Britton) leaf", J. Agric. Food Chem, 57, pp.11537~11542 (2009)[DOI]
  31. Tang WF, "Perilla (Perilla frutescens) leaf extract inhibits SARS-CoV-2 via direct virus inactivation", Biomed. J, 44, pp.293~303 (2021)[DOI]
  32. Taylor RH, "Descriptions of Plant Viruses", CMI, 1972
  33. Xia ZH, "First report of broad bean wilt virus 2 infecting Perilla frutescens in China", Plant Disease, 104, pp.3085~ (2020)[DOI]
  34. Yamasaki K, "Anti-HIV-1 activity of herbs in Labiatae", Biol. Pharm. Bull, 21, pp.829~833 (1998)

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