복구효소를 이용한 손상된 DNA의 STR 유전자형 분석
Application of a Repair Enzyme to STR Analysis of Damaged DNA
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DNA found at the scene of a crime is often damaged and degraded to small fragments and remained in low quantity. Therefore, there are some difficulties in forensic STR typing of these DNA samples. In this study, to overcome these limitations as different approach, we applied repairing enzyme to damaged DNA. The efficacy of a repair enzyme system (PreCR Repair Mix) was evaluated by using several types of artificially damaged and naturally damaged DNA. The results showed that autosomal STR amplification produced increased yield in the DNA damaged by UV irradiation,oxidation, or acid/heat, and DNA from dried blood spot and dried saliva spot by treatment of a repair enzyme, but not in DNA extracted from old skeletal remains. In conclusion,a repair enzyme will be efficiently applied to forensic samples which were damaged by UV irradiation, oxidation, and acid/heat.
Abstract
DNA found at the scene of a crime is often damaged and degraded to small fragments and remained in low quantity. Therefore, there are some difficulties in forensic STR typing of these DNA samples. In this study, to overcome these limitations as different approach, we applied repairing enzyme to damaged DNA. The efficacy of a repair enzyme system (PreCR Repair Mix) was evaluated by using several types of artificially damaged and naturally damaged DNA. The results showed that autosomal STR amplification produced increased yield in the DNA damaged by UV irradiation,oxidation, or acid/heat, and DNA from dried blood spot and dried saliva spot by treatment of a repair enzyme, but not in DNA extracted from old skeletal remains. In conclusion,a repair enzyme will be efficiently applied to forensic samples which were damaged by UV irradiation, oxidation, and acid/heat.
참고문헌 (18)
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