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배양된 C6 신경교종세포의 세포생존능과 항산화 효과에 순천만에서 자란 퉁퉁마디의 물리적활성의 연구

A Study on the Physioactivities of Salicornia herbacea L. Grown in Sunchon Bay on Cell Viability and Antioxidative Effect in Cultured C6 Glioma Cells

서영미|박승택|김신무|제갈승주|임요섭
대한임상검사과학회 (Korean Society for Clinical Laboratory Science)|2011.09|대한임상검사과학회지 (Korean Journal of Clinical Laboratory Science)|제43권 제3호|pp. 98-104

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Abstract

To evaluate the physioactivity of Salicornia herbacea L. (SH), which are obtained from Sunchon bay as wild plants, an SH extract was prepared by freeze drying to obtain SH, and by cold drying to obtain SH. For the evaluation of their bioactivities, cell viability and antioxidative effect were measured. The XTT assay was adopted to measure cell viability after C6 glioma cells were treated with various concentrations of reactive oxygen species (ROS) and hydrogen peroxide (H2O2) for 8 hours. The DPPH-radical scavenging activity was also measured for the antioxidative effect. In this study, the XTT50 value of H2O2 was determined at 30 μM which was highly toxic based on the cytotoxic criteria by Borenfreund and Puerner. The protective effect of SH extract significantly increased cell viability compared with H2O2-treated group. Its antioxidative effect showed a significant DPPH-radical scavenging activity at concentrations of 1-100 μg/mL, while SH extract showed highly a DPPH-radical scavenging activity at only 100 μg/mL. From these results, H2O2 was highly toxic in cultured C6 glioma cells, and SH extract was effective in the prevention of cell damage by its antioxidative effect.

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