SPME-GC 방법에 의한 프탈레이트류 분석에 따른 토양성분의 비교 및 출처 추정
Comparison of soil composition and source estimation according to the phthalate esters analysis by SPME-GC method
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
사건·사고와 관련된 토양을 가해자의 의류 또는 자동차 타이어 등에서 발견된 토양과의 비교 분석으로 가해자의 범죄 관련성을 입증할 수 있다. 그러나 토양은 다양한 종류의 광물들과 유기물로 조성되어 있기 때문에 광물의 비교만으로는 동일 출처 여부를 판단하기 어렵다. 따라서 인간의 활동과 함께 토양에 오염되어 분포되는 프탈레이트류는 토양 광물의 결과를 보완할 수 있는 좋은 지표물질이 될 수 있다. 주요 프탈레이트성분으로 디이소프탈레이트 (DIBP), 디 사이클로헥실프탈레이트(DCHP) 및 디에틸헥실프탈레이트(DEHP)를 밭, 논, 등산로, 운동장 등에서 채취한 토양에서 고체상미량추출-가스크로마토그래프(SPME-GC)로 분석하였다. 직선성(R2)은 약 0.986~0.996, 검출한계는 약 0.003~0.030 μg/g이었고, 채취지역에 따라 검출된 프탈레이트류의 종류와 검출량이 상이하였다. 산속의 토양에서는 프탈레이트류가 검출되지 않는 것으로 보아 토양의 출처 추정에서 오염지역과 비오염지역의 토양 여부를 판단할 수 있을 것으로 추정된다.
Abstract
Soil from a crime scene can be analyzed for its association with a perpetrator's crime through a comparative analysis of soil found on the perpetrator's clothing or the tires of a vehicle. However, due to the diverse mineral and organic composition of soil, determining if soil comes from the same source based solely on mineral comparison can be challenging. Therefore, phthalate esters present in the soil along with human activities can serve as a useful indicator to complement soil mineral results. Solid-phase microextraction-gas chromatography (SPME-GC) was used to analyze diisobutyl phthalate (DIBP), diethylhexyl phthalate (DEHP), and diethylhexyl phthalate (DCHP) in soil from potential crime locations such as fields, paddies, hiking trails, and playgrounds. The linearity (R2) ranged from approximately 0.986 to 0.996, and the detection limits were around 0.003 to 0.030 μg/g. Depending on the collection area, the kinds and amounts of detected phthalate esters varied. Phthalate esters were not detected in soils from deep mountain areas, which could potentially allow for distinguishing between contaminated and uncontaminated areas and estimating the origin of the soil for forensic purposes.
참고문헌 (36)
- TJ Hopen, "The value of soil evidence. Trace Evidence Analysis: More Cases in Forensic Microscopy and Mute Witnesses", pp.105~122 (2003), Elsevier Academic Press
- AM Bowen, "Forensic provenance investigations of soil and sediment samples", Geological Society Special Publications, 384(1), pp.9~25 (2013)
- CE Dong, "Strengths, Limitations, and Recommendations for Instrumental Color Measurement in Forensic Soil Characterization", J Forensic Sci, 65(2), pp.438~449 (2020)
- N Petraco, "Case studies in forensic soil examinations", Forensic Sci Int, 178(2-3), pp.e23~e27 (2008)
- K Pye, "Particle size analysis of sediments, soils and related particulate materials for forensic purposes using laser granulometry", Forensic Sci Int, 144(1), pp.19~27 (2004)
- T Kettler, "Simplified method for soil particle-size determination to accompany soil-quality analyses", Soil Sci Soc Am J, 65(3), pp.849~852 (2001)
- RJ Cox, "The forensic analysis of soil organic by FTIR", Forensic Sci Int, 108(2), pp.107~116 (2000)
- B Woods, "Soil examination for a forensic trace evidence laboratory-Part 2 : Elemental analysis", Forensic Sci Int, 245, pp.195~201 (2014)
- K Pye, "Elemental analysis of soil samples for forensic purposes by inductively coupled plasma spectrometry-precision considerations", Forensic Sci Int, 160(2-3), pp.178~192 (2006)
- S Giampaoli, "The environmental biological signature : NGS profiling for forensic comparison of soils", Forensic Sci Int, 240, pp.41~47 (2014)
- LI Moreno, "Microbial metagenome profiling using amplicon length heterogeneity-polymerase chain reaction proves more effective than elemental analysis in discriminating soil specimens", J Forensic Sci, 51(6), pp.1315~1322 (2006)
- A Chabbi, "Stable carbon isotope signature and chemical composition of organic matter in lignite-containing mine soils and sediments are closely linked", Org Geochem, 38(6), pp.835~844 (2007)
- J Liu, "A spatial distribution-Principal component analysis(SD-PCA)model to assess pollution of heavy metals in soil", Sci Total Environ, 859(Pt 1), pp.160112~ (2023)
- VF Melo, "Factor analysis of organic soils for site discrimination in a forensic setting", Forensic Sci Int, 290, pp.244~250 (2018)
- J Tekel, "Pesticide residue analyses in plant material by chromatographic methods : clean-up procedures and selective detectors", J Chromatogr A, 754(1-2), pp.397~410 (1996)
- C Li, "Phthalate esters in soil, plastic film, and vegetable from greenhouse vegetable production bases in Beijing, China : Concentrations, sources, and risk assessment", Sci Total Environ, 568, pp.1037~1043 (2016)
- B Zhou, "Contamination and human health risks of phthalate esters in vegetable and crop soils from the Huang-Huai-Hai region of China", Sci Total Environ, 778, pp.146281~ (2021)
- CA Staples, "Phthalate esters", pp.3~5 (2003), Springer Science & Business Media
- 백경민, "여수·광양지역 대기 중 입자상 다환방향족탄화수소와 프탈레이트화합물의 오염특성", 한국대기환경학회지, 35(6), pp.767~785 (2019), 한국대기환경학회[DOI]
- R Daňková, "Analysis of Phthalate Presence in Agricultural Soils in the Czech Republic", Pol J Environ Stud, 25(1), pp.443~446 (2016)
- FL Mayer, "Phthalate esters as environmental contaminants", Nature, 238(5364), pp.411~413 (1972)
- R Sugita, "Validity of color examination for forensic soil identification", Forensic Sci Int, 83, pp.201~210 (1996)
- K Pye, "Elemental analysis of soil samples for forensic purposes by inductively coupled plasma spectrometry-precision consideration", Forensi Sci Int, 160(2-3), pp.178~192 (2006)
- A Hu, "Simultaneous determination of phthalate diesters and monoesters in soil using accelerated solvent extraction and ultra-performance liquid chromatography coupled with tandem mass spectrometry", J Chromatogr A, 1626, pp.461347~ (2020)
- 김은호, "SPME-GC", MS의한 토양내 프탈레이트류 분석에 따른 법과학적 구별, 2012, pp.6(3), 190~197[DOI]
- DJ Russell, "Analysis for phthalate esters in environmental samples : separation from PCB's and pesticides using dual column liquid chromatography", Int J Environ Ana Chem, 15(3), pp.165~183 (1983)
- H Yan, "Simultaneous determination of four phthalate esters in bottled water using ultrasound-assisted dispersive liquid–liquid microextraction followed by GC-FID detection", Analyst, 135(10), pp.2585~2590 (2010)
- MD Carlo, "Determination of phthalate esters in wine using solid-phase extraction and gas chromatography–mass spectrometry", Food Chem, 111(3), pp.771~777 (2008)
- P Liu, "Assessment and analysis of phthalate esters, in Lake Pontchartrain, by SPME combining with GC-MS", Environ technol, 34(4), pp.453~462 (2013)
- MV Russo, "Extraction and GC-MS analysis of phthalate esters in food matrices : a review", Rsc Adv, 5(46), pp.37023~37043 (2015)
- KC Ting, "GC", MS screening method for phthalate esters in children's toys, 2009, pp.92(3), 951~958
- R Aparicio-Ruiz, "Comparison of two analytical methods validated for the determination of volatile compounds in virgin olive oil : GC-FID vs GC-MS", Talanta, 87, pp.133~141 (2018)
- AC Werner, "Vapor pressures of phthalate esters", Ind Eng Chem, 44(11), pp.2736~2740 (1952)
- AB Fialkov, "A new type of GC–MS with advanced capabilities", Intl J Mass Spectrom, 251(1), pp.47~58 (2006)
- CN Huang, "Children's exposure to phthalates in dust and soil in Southern Taiwan : A study following the phthalate incident in 2011", Sci Total Environ, 696, pp.133685~ (2019)
- J Krupčík, "Considerations on the determination of the limit of detection and the limit of quantification in one-dimensional and comprehensive two-dimensional gas chromatography", J Chromatogr A, 1396, pp.117~130 (2015)