SPME-GC/MS의한 토양내 프탈레이트류 분석에 따른 법과학적 구별
Discrimination of Forensic Soils with Phthalates Obtained by SPME-GC/MS
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초록
토양에 함유된 프탈레이트류 성분들은 살인, 강간, 농작물 절도 및 교통사고 등의 각종 사건·사고에서 토양의 동일성 비교 또는 출처를 추정하는데 있어 지표물질로 사용될 수 있을 것으로 나타났다. 지역에 따라 토양에 함유된 프탈레이트의 종류와 함량에 있어 차이가 있는 지를 검토하기 위하여 한강유역, 하천주변, 주택지, 운동장, 가로수 그리고 등산로에서 채취한 토양에서 8종의 프탈레이트를 SPMEGC/MS로 분석하였다. 8종의 프탈레이트로는 디메틸프탈레이트, 디에틸프탈레이트, 이이소부틸프탈레이트, 디부틸프탈레이트, 디헥실프탈레이트, 디시클로헥실프탈레이트 및 디에틸헥실 프탈레이트로서 SPMEGC/MS 분석 시 폴리아크릴레이트 fiber가 가장 좋은 흡착률과 분리능을 보였다. 이 분석법은 양호한 직선성 R^2= 0.994 ~ 0.998을 나타내었으며, 8종의 프탈레이트류에 대한 검출한계와 정량한계는 각각 0.02~0.5 ng/g 및 0.08 ~ 1.35 ng/g이였다. 프탈레이트의 종류와 함량은 채취한 지역에 따라 차이가 나타나는 것으로 보아 법과학적으로 토양의 동일성여부 및 출처를 판단하는 표지물질로서 유용하게 응용될 수 있을 것으로 판단되었다.
Abstract
It is proved that phthalates contained in soils could be markers used to compare and assess the origin of soils related to murder, rape, crop-theft cases and traffic accidents. Soil samples were prepared from the Han-riversides, streams, residential sections, playgrounds, street trees and mountain roads to find out whether there was any difference in the kind and level of phthalates. Eight phthalates, such as dimethyl phthalate, diethyl phthalate, diisobutyl phthalate, dibutyl phthalate, dihexyl phthalate, dicyclohexyl phthalate and diethylhexyl phthalate, were analyzed by solid phase microextraction-gas chromatography/mass spectrometry (SPME-GC/MS), which showed that polyacrylate fiber was best in adsorption efficiency and resolution. This method showed a good linearity (R^2=0.994 ~ 0.998) and the limits of detection and quantification for the 8 phthalates were about 0.02 ~ 0.5 ng/g and 0.08 ~ 1.35 ng/g, respectively. There was a significant degree of difference in the kinds of phthalates vis a vis their location of origin, regardless of the types of samples. Thus, phthalates could be applied in forensic investigations. Principal components analysis was optimistically applied to discriminate soil samples distinctively.
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