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북방산개구리(Rnan dybowskii )유생의 포식자회피 행동에 대한 FNW와FNW-Ag 복합체의 영향

Effects of FNW and FNW-Ag on the Antipredator Behavior of Dybowski’s Frog (Rana dybowskii) Larvae

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키워드

주제분류기타자연과학

초록

기존의 독성평가는 생물의 생존가능성에 영향을 줄 수 있는 이상행동반응에 대한 평가를 거의 하지 않았다. 따라서 본 연구에서는행동학적 이해를 바탕으로 양서류 유생들의 생존가능성과 직결되는 포식자회피반응 행동을 이용하여 fullerene nanowhisker(FNW)와 nanowhisker-silver nanoparticle composites(FNW-Ag복합체)의 독성을 평가해 보았다. 우리는 나노물질에 노출되지 않는 그룹과 5가지 농도에 따른 나노물질에 노출되는 그룹(FNW 10㎍/ml, 100㎍/ml, 500㎍/ml과 FNW-Ag복합체10㎍/ml, 50㎍/ml)으로 나누어 실험하였다. 그 결과, FNW-Ag복합체 50㎍/ml농도에 노출된 유생들을 제외하고는 평소활동량에는 차이가 없었지만, FNW 10㎍/ml농도에 노출된 유생들을 제외하고는 모두 포식자인식 반응에 따른 활동변화에서는통계적으로 유의한 차이가 나타났다. 즉, 나노물질노출 유무는 평소 움직임에는 영향을 주지 않지만, 행동학적 이해를 바탕으로한 분석결과 포식자회피반응에 영향을 준다는 것을 알 수 있었다. 따라서 생물을 대상으로 하는 독성평가연구에 행동학적분석이 이루어져야 할 것으로 판단된다.

Abstract

Existing toxicity assessment researches were rarely studied on assessment of the abnormal behavior that affecting survival. Therefore, the study used anti-predator response of amphibians larvae, based on behavioral understanding, to assess toxicity in fullerene nanowhisker(FNW) and fullerene nanowhisker-silver nanoparticle composites(FNW-Ag). The experiment was conducted by dividing the groups not exposed to nanomaterials and exposed to nanomaterials at five concentrations(FNW: 10㎍/ml, FNW: 100㎍/ml, FNW: 500㎍/ml FNW-Ag: 10㎍/ml, FNW: 50㎍/ml). As a result, there were no differences in normal activity except those exposed to concentrations of FNW-Ag 50 ㎍/ml, but there were statistically significant differences in anti-predator response except those exposed to concentrations of FNW 10 ㎍/ml. That is, exposed of nanomaterials does not affect ordinary movements, but analyses based on behavioral understandings have shown that it has an influence on the anti-predator response. It is therefore considered necessary to have animal behavioral analysis method performed in the assessment of eco-toxic experiment.

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