고삼뿌리 추출물 내 Kurarinone, Oxymatrine, Matrine 분석 방법 검증
Analytical Method Validations for Kurarinone, Oxymatrine and Matrine in Sophora flavescens Root Extract
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The root of the Sophora flavescens with excellent pharmacological efficacy has been used as a herbal medicine. The test was carried out to verify the analysis of kurarinone, oxymatrine and matrine in the root extract of the Sophora flavescens using HPLC. As a result of confirming the MS/MS by fractionation, it was confirmed that the corresponding peaks were kurarinone, oxymatrine, and matrine, respectively, as the MS/MS spectrum was consistent. As a result of confirming the accuracy test and reproducibility, and the precision within and between batches, it was possible to confirm high accuracy and precision. As a result of confirming the stability of the pre-treated sample, all three materials were stable for 48 hours. In conclusion, it was evaluated and established that the method validation for kurarinone, oxymatrine and matrine in the range with the specificity, linearity, accuracy, precision, and stability.
Abstract
The root of the Sophora flavescens with excellent pharmacological efficacy has been used as a herbal medicine. The test was carried out to verify the analysis of kurarinone, oxymatrine and matrine in the root extract of the Sophora flavescens using HPLC. As a result of confirming the MS/MS by fractionation, it was confirmed that the corresponding peaks were kurarinone, oxymatrine, and matrine, respectively, as the MS/MS spectrum was consistent. As a result of confirming the accuracy test and reproducibility, and the precision within and between batches, it was possible to confirm high accuracy and precision. As a result of confirming the stability of the pre-treated sample, all three materials were stable for 48 hours. In conclusion, it was evaluated and established that the method validation for kurarinone, oxymatrine and matrine in the range with the specificity, linearity, accuracy, precision, and stability.
참고문헌 (20)
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