황호접 추출물의 항산화, 항염, 미백 및 수렴 효능 평가
Anti-oxidant, Anti-inflammatory, Whitening, and Astringent Effect of Senna bicapsularis Extracts
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초록
본 연구는 황호접(Senna bicapsularis) 나무를 사용하여 기능성화장품 소재로 활용 가능성을 조사하고자 황호접 추출물을 제조한 후, 이를 대상으로 항산화, 미백, 항염 및 수렴 효과 등의 생물학적 특성을 평가하였다. 황호접 잎 80%에탄올추출물을 SBL (S. bicapsularis leaf 80% ethanol extract), 꽃 80%에탄올추출물을 SBF (S. bicapsularis flower 80% ethanol extract)로 명명하였다. SBL은 낮은 IC50 값을 나타냈으며, 높은 DPPH, ABTS 라디컬 소거 활성이 확인되었다. 총포리페놀 함량 측정 결과, SBF는 28.191±0.387 mgGAE/g, SBL은 140.931±0.581 mgGAE/g로 SBL 내 폴리페놀 함량이 매우 높은 것으로 확인되었다. 항염 효능은 LPS로 RAW264.7 cell에 염증을 유도한 후 세포 독성이 없는 추출물 농도 조건하에서 수행하였다. 그 결과, NO 생성, iNOS 단백질 발현 및 염증성 싸이토카인 (TNF-α and IL-6) 분비량은 SBL 농도 의존적으로 억제되었다. 황호접 잎 추출물은 mushroom tyrosinase 활성과 DOPA 산화를 저해하였으며, 탄닌-헤모글로빈 결합 반응을 통한 수렴 활성을 나타냈다. 이러한 결과들을 통해 황호접 추출물은 천연소재의 항산화, 항염, 미백, 및 수렴 효능이 있는 기능성화장품 원료로써 활용 가능성이 높다고 판단된다.
Abstract
The aim of this study was to assure the possibility of using Senna bicapsularis as a functional cosmetic material. For this, we carried out biologically characteristic evaluations about anti-oxidant, whitening, anti-inflammatory, and astringent effects using S. bicapsularis extract. To carry out this experiment, we prepared S. bicapsularis extract from leaf 80% ethanol extract (SBL) and flower 80% ethanol extract (SBF). SBL showed the highest DPPH and ABTS radical scavenging activities with a minimum IC50 value. Compared to the SBF (28.191±0.387 mgGAE/g), the SBL (140.931±0.581 mgGAE/g) showed a greater phenolic content. The inflammatory activity effect in SBL and SBF was confirmed in LPS-induced mouse macrophage RAW264.7 cells. On cell viability, SBL and SBF had no toxicity because it showed a high cell viability at a high concentration. SBL significantly decreased the production of Nitric oxide (NO) production and inducible NO synthase in LPS-induced RAW264.7 cells. Additionally, SBL decreased pro-inflammatory cytokines such as Tumor necrosis factor-α (TNF-α) and Interleukin-6 (IL-6) in a concentration-dependent manner. For anti-melanogenesis effect, tyrosinase inhibition activity was assessed by using an in vitro mushroom tyrosinase inhibition assay and DOPA (Dihydroxyphenylalanine) oxidation inhibition assay. SBL inhibited the tyrosinase inhibition activity in a dose-dependent manner. SBL and SBF suppressed the DOPA oxidation with 19.391%, 14.726%, respectively. Moreover, SBL exhibited astringent effects on tannin-hemoglobin binding reaction. Therefore, the results indicate that S. bicapsularis extract can play a key role as a natural material and a functional cosmetic ingredient for anti-oxidant, anti-inflammatory, whitening, and astringent effect.
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