탱자나무열매와 유효성분인 나린진의 항노화 활성 연구
A Study on the Anti-Aging Activity of Poncirus trifoliata Fruit and Nargin, an Active Ingredient
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본 연구에서는 탱자나무열매 추출물의 잠재적인 노화 방지 특성을 항산화 및 항산화 효과에 대한 효능에 초점을 맞추어 조사했다. 탱자나무열매추출물과 나린진은 DPPG(음이온성), ABTS(양이온성) 라디칼 소거 활성과 SOD-Llike에서 농도의존적 활성을 보였다. 또한, 탱자나무열매 추출물과 나린진은 최대 500 µg/ml 농도에서 섬유아세포에서 세포독성을 나타내지 않았고, 증식 활성을 나타내지 않았다. 또한, 탱자나무열매 추출물과 나린진은 콜라겐 분해 역할로 인해 피부 노화와 관련된 핵심 활성인 MMP-1활성 억제를 보였다. 탱자나무열매 추출물은 최대 500 µg/ml에서 MMP-1 발현을 74.93% 억제한 반면, 나린진은 동일한 농도에서 MMP-1 발현을 88.51% 억제했다. 결론적으로, 이번 연구 결과는 탱자나무열매 과일 추출물과 그 활성 성분인 나린진의 잠재적인 유용성을 강조하여 피부 탄력성을 강화하는 효과적인 기능적 재료로서 항노화 피부 관리 제형에서 효과적일 것이라 전망한다.
Abstract
In this study, the potential anti-aging properties of Poncirus tripoliata fruit extract were investigated, especially focusing on its anti-oxidant effect and anti-aging effect. In addition, the anti-aging effect of the skin was investigated by naringin, an active ingredient in Poncirus tripoliata fruit extract. First, to check the antioxidant power, we conducted a study on the elimination of DPPH(2,2-diphenyl-1-pyrylhydrazyl) and ABTS(2,2′-azino-bis(3-ethylbenzoizolin-6-sulfonic acid) radicals. In particular, both Poncirus tripoliata fruit extract and naringin exhibited concentration-dependent antioxidant effects, confirming their efficacy in neutralizing active radicals. In addition, the evaluation of superoxide disutase(SOD)-like activity also confirmed the anti-oxidant effect in a concentration-dependent manner. In particular, the Poncirus tripoliata fruit extract and naringin did not show cytotoxicity in fibroblasts at concentrations up to 500 µg/ml, and cell proliferation was significantly increased by 13.23% at this concentration threshold. Furthermore, we investigated the effects of Poncirus tripoliata fruit extract and naringin on the expression of matrix metaloloproteinase-1(MMP-1), a key enzyme involved in collagen degradation. The Poncirus tripoliata fruit extract inhibited MMP-1 expression by 74.93% at a maximum concentration of 500 μg/ml, whereas naringin inhibited MMP-1 expression by 88.51% at the same concentration threshold. In conclusion, in this study, evaluation of Poncirus tripoliata fruit extract and naringin, an efficacy component, confirmed the potential efficacy on strengthening skin elasticity, and we believe that these results will play a particularly surprising role in preventing skin aging. Based on these findings, we believe that they can be applied to future clinical studies and the development of functional materials.
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