황금추출물과 용매분획물의 항균활성 및 항염효과에 관한 연구
A Study on the Antimicrobial Activity and Anti-inflammatory Effect of Scutellaria baicalensis Extract and Solvent Fraction
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연구의 목적은 황금 추출물의 항균과 항염증 효과를 연구하고 이를 화장품 원료로서의 사용 가능성을 탐구하는 것이다. 황금 추출물은 70% 에탄올로 추출되었고 유기 용매로 분획되었다. 황금 추출물과 그 분획물은 Propionibacterium acnes(P.acnes) 및 Malassezia furfur(M.furfur)의 항균 활성을 테스트하였다. RAW 264.7 대식세포를 이용하여 황금추출물의 항염증 활성을 NO 분석 및 췌장 리파아제 억제 효과를 통해 평가되었다. 실험결과 황금 추출물과 그 분획물은 P.acnes 및 M.furfur의 항균 활성에 대해 명확한 결과가 나타났으며, 최소 억제 농도는 약 10,000 μg/mL로 확인되었다. 또한 황금 추출물과 그 분획물은 1,000 μg/mL까지 강력한 췌장 리파아제 억제 활성을 나타내었다. 황금 추출물과 그 분획물은 50 μg/mL까지 세포독성을 보이지 않았으며, LPS에 의해 발현된 NO는 RAW 264.7 세포 내 농도 의존적으로 감소되었다. 이러한 결과에 통하여, 황금 추출물은 중요한 항균 및 항염증 효과를 나타내어 화장품산업의 기능성 원료로 개발될 수 있음을 확인 할 수 있었다.
Abstract
The objective of this study was to investigate the antibacterial and anti-inflammatory properties of gold extract and explore its potential use as a cosmetic ingredient. The Scutellaria baicalensis extract was obtained using 70% ethanol extraction and fractionated with organic solvents. Antibacterial activity against Propionibacterium acnes (P. acnes) and Malassezia furfur (M. furfur) was tested for both the Scutellaria baicalensis extract and its fractions. Anti-inflammatory activity was evaluated through pancreatic lipase inhibition and analysis of nitric oxide (NO) production in RAW 264.7 cells. the results showed that the Scutellaria baicalensis extract and its fractions exhibited significant antibacterial activity against P. acnes and M. furfur, with minimum inhibitory concentrations confirmed at approximately 10,000 μg/mL. Furthermore, the Scutellaria baicalensis extract and fractions demonstrated potent pancreatic lipase inhibition activity up to 1,000 μg/mL. They showed no toxicity up to 50 μg/mL and dose-dependently reduced LPS-induced NO production in RAW 264.7 cells. these findings suggest that Scutellaria baicalensis extract exhibits important antibacterial and anti-inflammatory effects, indicating its potential development as a multifunctional cosmetic ingredient.
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