Fabrication of a polymerase chain reaction micro-reactor using infrared heating
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Abstract
A silicon-based micro-reactor to amplify small amount of deoxyribonucleic acid (DNA) has been fabricated using micro-electro-mechanical systems (MEMS) technology. Polymerase chain reaction (PCR) of DNA requires a precise and rapid temperature control. A Pt sensor is integrated directly in the chamber for real-time temperature measurement and an infrared lamp is used as external heating source for non-contact and rapid heating. In addition to the real-time temperature sensing, PCR needs a rapid thermocycling for effective PCR. For a fast thermal response, the thermal mass of the reactor chamber is minimized by removal of bulk silicon volume around the reactor using double-side KOH etching. The transparent optical property of silicon in the infrared wavelength range provides an efficient absorption of thermal energy into the reacting sample without being absorbed by silicon reactor chamber. It is confirmed that the fabricated micro-reactor could be heated up in less than 30 sec to the denaturation temperature by the external infrared lamp and cooled down in 30 sec to the annealing temperature by passive cooling.
참고문헌 (14)
- M. A. Northrup,M. T. Ching,R. M. White, "DNA amplication with a microfabricated reaction chamber", pp. 924-926, pp.1993~
- M. U. Kopp, "Continuous-flow PCR on a chip", 280, pp.1046~1048 (1998)
- J. H. Daniel, "Silicon microchambers for DNA amplification", 71, pp.81~88 (1998)
- A. Fuchs, "A silicon lab-on-chip for integrated sample preparation by PCR and DNA analysis by hybridization Int. Conf. on Microtechnologies in Medicine & Biology", pp.227~231 (2002)
- Y.-K. Kim, "The Trend of MEMS Technology", 8(5), pp.354 1999~358
- D. S. Yoon, "and G. B. Lim Precise temperature control and rapid thermal cycling in a micromachined DNA polymerase chain reaction chip", pp.813~823 (2002)
- S. L.-Esteban, "Bioactive glass coatings for orthopedic metallic implants", 23, pp.2921~2930 (2003)
- J. Ballarre, "In vivo and in vitro evaluation of vitreous coatings on cobalt base alloys for prosthetic devices", 304, pp.278~285 (2002)
- C. denBesten, "Polymer bonding of micro-machined silicon structures", 92, pp.104~107 (1992)
- J. E.-Ali, "Simulation and experimental validation of a SU-8 based PCR thermocycler chip with integrated heaters and temperature sensor", 110, pp.3~10 (2004)
- E. Kukharenka, "Electroplating moulds using dry film thick negative photoresist", 13, pp.69~74 (2003)
- K.-D. Song, "and D.-D. Lee Characteristics and fabrication of micro gas sensor with single electrode", 11(6), pp.350~357 (2002)
- A. F. R. Huhmer, "Noncontact infrared-mediated thermocycling for effective polymerase chain reaction amplification of DNA in nanoliter volumes", 72, pp.5507~5512 (2000)
- D. K. Schroder,R. NoelThomas,and J. C. Schwartz,, "Free carrier absorption in silicon", sc-13, pp.180~187 (1978)