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除濕胃苓湯加減이 집 먼지 진드기 추출물로 유도된 NC/Nga mouse의 아토피 피부염에 미치는 영향

Effect of Jeseupwiryeongtang-gagam on Atopic Dermatitis-like Skin NC/Nga Mouse Induced by Dermatophagoides Farinae Crude Extract

김윤희|한재경|나동규
대한한방소아과학회 (The Association of Pediatrics of Korean Medicine)|2008.08|대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)|제22권 제2호|pp. 81-114

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참고문헌

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Atopic dermatitisNC/Nga mouseJeseupwiryeongtang-gagam(JWTG)
주제분류한의학

초록

Objectives The purpose of this study is to investigate the effect of Jeseupwiryeongtang-gagam(JWTG) on atopic dermatitis by in vivo experiment using NC/Nga atopic dermatitis mouse, which has histological and clinical similarities to the atopic dermatitis of human. Methods To investigate the effect of JWTG on AD, we evaluated atopic dermatitis-like skin lesions by clinical skin index and analyzed immunological parameters in peripheral blood mononuclear cells(PBMCs), splenocytes, draining lymph node(DLN) and performed skin histology in ears and dorsal skin of atopic dermatitis-like skin NC/Nga mouse in vivo. Results and Conclusions In vivo, clinical skin severity score were significantly lower in JWTG group than control group. IgE, IL-6, TNF-α, IgM, IgG2a and IgG2b levels in serum were decreased remarkably in JWTG group than control group and IFN-γ production, secreted in Th1 cell were increased by JWTG. After experiment ended, we analyzed immunological cells (CD3+, CD19+, CD4+, CD8+, CD3+CD69+, CD4+CD25+ and CD49b+) by flow cytometry. It resulted that total absolute number of CD3+, CD19+, CD4+ and CD8+ cells were recovered as normal and CD3+CD69+ were decreased significantly compared with control group in isolated DLN and PBMCs from NC/Nga mouse and total absolute number of Gr-1+, CD11b+and CD3+ in dorsal skin of NC/Nga mouse were decreased by JWTG. We analyzed ear, DLN, and neck-back skin after biopsy and dyeing by hematoxyline/eosin(H&E) and toluidine staining (mast cells marker) and obtained results that JWTG were effective to histological symptoms (dermal and epidermal thickening, hyperkeratosis and inflammatory cell infiltration). Ear thickness was decreased significantly than the control group and the size of inflammatory lymphocytes cells(ILC) and plasma cells(PC) in DLN were also decreased.

Abstract

Objectives The purpose of this study is to investigate the effect of Jeseupwiryeongtang-gagam(JWTG) on atopic dermatitis by in vivo experiment using NC/Nga atopic dermatitis mouse, which has histological and clinical similarities to the atopic dermatitis of human. Methods To investigate the effect of JWTG on AD, we evaluated atopic dermatitis-like skin lesions by clinical skin index and analyzed immunological parameters in peripheral blood mononuclear cells(PBMCs), splenocytes, draining lymph node(DLN) and performed skin histology in ears and dorsal skin of atopic dermatitis-like skin NC/Nga mouse in vivo. Results and Conclusions In vivo, clinical skin severity score were significantly lower in JWTG group than control group. IgE, IL-6, TNF-α, IgM, IgG2a and IgG2b levels in serum were decreased remarkably in JWTG group than control group and IFN-γ production, secreted in Th1 cell were increased by JWTG. After experiment ended, we analyzed immunological cells (CD3+, CD19+, CD4+, CD8+, CD3+CD69+, CD4+CD25+ and CD49b+) by flow cytometry. It resulted that total absolute number of CD3+, CD19+, CD4+ and CD8+ cells were recovered as normal and CD3+CD69+ were decreased significantly compared with control group in isolated DLN and PBMCs from NC/Nga mouse and total absolute number of Gr-1+, CD11b+and CD3+ in dorsal skin of NC/Nga mouse were decreased by JWTG. We analyzed ear, DLN, and neck-back skin after biopsy and dyeing by hematoxyline/eosin(H&E) and toluidine staining (mast cells marker) and obtained results that JWTG were effective to histological symptoms (dermal and epidermal thickening, hyperkeratosis and inflammatory cell infiltration). Ear thickness was decreased significantly than the control group and the size of inflammatory lymphocytes cells(ILC) and plasma cells(PC) in DLN were also decreased.

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