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驅風除濕湯이 DNCB로 유도된 NC/Nga mice의 아토피 피부염에 미치는 영향

Effect of Gupoongjeseuptang (GPJST) on DNCB (dinitrochlorobenzene)-induced Atopic Dermatitis-like Model NC/Nga Mice

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초록

Objectives The purpose of this study is to investigate the effect of Gupoongjeseuptang (GPJST) on atopic dermatitis by in vivo experiment using NC/Nga atopic dermatitis mouse, which has histological and clinical similarities to the atopic dermatitis of human. Methods To investigate the effect of GPJST on atopic dermatifis, we evaluated atopic dermatitis-like skin lesions by clinical skin index and analyzed immunological parameters in peripheral blood mononuclear cells (PBMCs), splenocytes, draining lymph node (DLN) and performed skin histology in ears and dorsal skin of atopic dermatitis-like skin NC/Nga mouse in vivo. Results In vivo, clinical skin severity score were significantly lower in GPJST group than control group. IgE, IL-6, TNF-α, IgG1, IgM, IgG2a and IgG2b levels in serum decreased remarkably in GPJST group than control group. Also, total absolute number of CD3+CD69+, and CCR3+ cells recovered as normal in PBMCs and CD3+, CD3+CD69+ decreased significantly compared with control group in isolated DLN from NC/Nga mouse and total absolute number of CD11b+Gr-1+, CCR3+CD3+ in dorsal skin of NC/Nga mouse decreased by GPJST. We analyzed ear and neck-back skin after biopsy and dyeing by hematoxyline/eosin (H&E) and toluidine staining (mast cells marker) and obtained results that GPJST are very effective to histological symptoms (dermal and epidermal thickening, hyperkeratosis and inflammatory cell (CD4, CCR3+) infiltration). Conclusions This study demonstrates immunological activity of GPJST on atopic dermatitis-like model mice.

Abstract

Objectives The purpose of this study is to investigate the effect of Gupoongjeseuptang (GPJST) on atopic dermatitis by in vivo experiment using NC/Nga atopic dermatitis mouse, which has histological and clinical similarities to the atopic dermatitis of human. Methods To investigate the effect of GPJST on atopic dermatifis, we evaluated atopic dermatitis-like skin lesions by clinical skin index and analyzed immunological parameters in peripheral blood mononuclear cells (PBMCs), splenocytes, draining lymph node (DLN) and performed skin histology in ears and dorsal skin of atopic dermatitis-like skin NC/Nga mouse in vivo. Results In vivo, clinical skin severity score were significantly lower in GPJST group than control group. IgE, IL-6, TNF-α, IgG1, IgM, IgG2a and IgG2b levels in serum decreased remarkably in GPJST group than control group. Also, total absolute number of CD3+CD69+, and CCR3+ cells recovered as normal in PBMCs and CD3+, CD3+CD69+ decreased significantly compared with control group in isolated DLN from NC/Nga mouse and total absolute number of CD11b+Gr-1+, CCR3+CD3+ in dorsal skin of NC/Nga mouse decreased by GPJST. We analyzed ear and neck-back skin after biopsy and dyeing by hematoxyline/eosin (H&E) and toluidine staining (mast cells marker) and obtained results that GPJST are very effective to histological symptoms (dermal and epidermal thickening, hyperkeratosis and inflammatory cell (CD4, CCR3+) infiltration). Conclusions This study demonstrates immunological activity of GPJST on atopic dermatitis-like model mice.

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