理中湯이 IgE가 과잉생성되고 피부염이 유발된 NC/Nga생쥐에서 IFN-γ 반응에 의한 Th2 세포분화 억제에 미치는 영향
The Effect of Li Zhong Tang on the Suppression of Th2 Differentiation by IFN-γ Response in IgE Hyperproduction and Atopic Dermatitis-like Skin Lesions Induced NC/Nga Mouse
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Objectives The purpose of this study is to investigate the effect of Li Zhong Tang(LZH-T) on atopic dermatitis by using NC/Nga atopic dermatitis mouse. Methods First, in vitro, we isolated B cells from NC/Nga mouse which induced atopic dermatitis-like skin for 18 weeks. We analyzed FACS by intracellular staining of IFN-γ, GATA-3+ and also analyzed cytokines by using real-time PCR. Secondly, in vivo , after administration of LZH-T to the 12 weeks old mouse with atopic dermatitis. We analyzed serum IgE, IFN-γ level and observed the changes of activated cell. Results In vitro, LZH-T decreased the levels of CD4+/IFN-γ+ and increased the levels of CD4+/GATA3+. In vivo, serum IgE levels were decreased and IFN-γ levels were increased in LZH-T group compared to the control group. In PBMCs, the percentage of activated cell - granulocytes, CD3+, CD3+CD4+, B220+CD23+, and CCR3+ were decreased and CD19+, CD3+CD8+ were increased in LZH-T group compared to the control group. Conclusions This study demonstrates immunological activity of GPJST on atopic dermatitis-like model mice.
Abstract
Objectives The purpose of this study is to investigate the effect of Li Zhong Tang(LZH-T) on atopic dermatitis by using NC/Nga atopic dermatitis mouse. Methods First, in vitro, we isolated B cells from NC/Nga mouse which induced atopic dermatitis-like skin for 18 weeks. We analyzed FACS by intracellular staining of IFN-γ, GATA-3+ and also analyzed cytokines by using real-time PCR. Secondly, in vivo , after administration of LZH-T to the 12 weeks old mouse with atopic dermatitis. We analyzed serum IgE, IFN-γ level and observed the changes of activated cell. Results In vitro, LZH-T decreased the levels of CD4+/IFN-γ+ and increased the levels of CD4+/GATA3+. In vivo, serum IgE levels were decreased and IFN-γ levels were increased in LZH-T group compared to the control group. In PBMCs, the percentage of activated cell - granulocytes, CD3+, CD3+CD4+, B220+CD23+, and CCR3+ were decreased and CD19+, CD3+CD8+ were increased in LZH-T group compared to the control group. Conclusions This study demonstrates immunological activity of GPJST on atopic dermatitis-like model mice.
참고문헌 (57)
- 박용민, "아토피피부염 병태생리에 대한 최신 지견", 대한소아알레르기및호흡기학회지, 16(3), pp.189~196 (2006), 대한 소아알레르기 호흡기학회
- 이해성, "소아 아토피 피부염의 빈도와 원인의 변화 - 10년 전과 비교하여 -", 대한소아알레르기및호흡기학회지, 12(4), pp.263~281 (2002), 대한 소아알레르기 호흡기학회
- Halbert AR, "Atopic dermatitis : Is it an allergic disease", J Am Acad Dermatol, 33, pp.1008~1018 (1995)
- Leung DY, "Atopic dermatitis", Lancet, 361, pp.151~160 (2003)
- Renz H, "Enhanced IL-4 production and IL-4 receptor expression in atopic dermatitis and their modulation by interferon-gamma", J Invest Dermatol, 99, pp.403~ (1992)
- 강재성, "세포분자면역학.제5판", pp.268~ (2005), 범문사
- 陳實功, "外科正宗", pp.269~ (1983), 人民衛生出版社
- 홍원식, "정교황제내경소문", pp.72~80 (1985), 동양의학연구원출판부
- 宋書功, "金匱要略 廣州校詮", pp.221~ (1994), 人民衛生出版社
- 吳謙, "의종금감(하)", pp.443~ (1982), 人民衛生出版社
- 전국 한의과대학 소아과학교실, "한방소아과학강의록", pp.606~611 (2001), 누리기획
- 이원세, "新方新編", pp.114~ (2001), 대성의학사
- 진경선, "淸上防風湯의 아토피성 알레르기 반응 조절 효과", 대한한방소아과학회지, 18(2), pp.61~76 (2004)
- 유한정, "內消和中湯加味方이 아토피피부염 환아의 혈중 Cytokine 분비에 미치는 영향", 대한한방소아과학회지, 20(1), pp.15~29 (2006), 대한한방소아과학회
- 김홍배, "升麻葛根湯의 항히스타민 효과 및 IL-4와 GM-CSF 발현에 미치는 영향", 대한한방소아과학회지, 20(1), pp.69~83 (2006), 대한한방소아과학회
- 박성희, "脾虛蘊濕方이 아토피 피부염 유발 NC", Nga 생쥐의 혈액내 면역 관련 인자에 미치는 영향, 2007, pp.21(4), 849~855[DOI]
- 윤재은, "驅風除濕湯이 DNCB로 유도된 NC", Nga mice의 아토피 피부염에 미치는 영향, 2008, pp.22(3), 105~137[DOI]
- 박두병, "凉血除濕湯이 DNCB로 유도된 NC", Nga mice의 아토피 피부염에 미치는 영향, 2009, pp.23(3), 263~291[DOI]
- 윤지연, "加味淸心蓮子湯이 NC", Nga mice의 아토피樣 피부염에 미치는 영향, 2007, pp.21(1), 87~116[DOI]
- 조백건, "內消和中湯加味方의 아토피 피부염 치료에 대한 임상고찰", 대한한방소아과학회지, 19(1), pp.131~141 (2005), 대한한방소아과학회
- 나동규, "除濕胃苓", b>加, Nga mouse의 아토피 피부염에 미치는 영향, pp.대한한방소아과학회지, 2008[DOI]
- Skehan P, "New colorimetric cytotoxicity assay for anticancer drug screening", J Natl Cancer Inst, 82(13), pp.1107~1112 (1990)
- Matsuda, H., N, "Development of atopic dermatitis like skin lesion with IgE hyperproduction in NC", Nga mice, 1997, pp.9, 461~466
- Sturgil S, "Atopic dermatitis update", Curr Opin Pediatr, 16, pp.396~401 (2004)
- Wollenberg A, "Atopic dermatitis : pathogenetic mechanism", Clin Exp Dermatol, 25, pp.530~534 (2000)
- Marsh DG, "Linkage analysis of IL-4 and other chromosome 5q31.1 markers and total serum immunoglobulin E concentrations", Science, 264, pp.1152~ (1994)
- 안성구, "아토피 피부염", pp.32~ (2007), 퍼시픽출판사
- Kevin DC, "Atopic dermatitis: recent trends in pathogenesis and therapy", J invest Dermatol, 102, pp.128~137 (1994)
- Horsmanheimo L, "Mast cells are one major source of interleukin-4 in atopic dermatitis", Br J Dermatol, 131, pp.348~353 (1994)
- Patrick D, "Does atopic dermatitis result from cytokine dysregulation?", J invest Dermatol, 102, pp.741~ (1994)
- Foster PS, "Interleukin-4, -5, and -13 : emerging therapeutic targets in allergic disease", Phamacology & Therapeutics, 94, pp.253~264 (2002)
- 노건웅, "아토피피부염의 감마 인터페론 치료", 소아알레르기 및 호흡기 학회지, 7(2), pp.239~247 (1998)
- 김세종, "IMMUNOLOGY", pp.1~ (1994), 고려의학
- 오재원, "집먼지진드기에 감작된 아토피 및 비아토피 개체에서 Derp Ⅰ 및 면역변환제의 자극에 의한 T 림프절구의 Interleukin-4와 Interferon -γ 생성의 차이", 천식과 알레르기, 19(4), pp.548~556 (1999)
- 김정원, "알레르기 및 면역학적 관점에서의 아토피피부염", 대한피부과학회지, 41(6), pp.687~689 (2003)
- Renz H, "Enhanced IL-4 production and IL-4 receptor expression in atopic dermatitis and their modulation by interferon-gamma", J Invest Dermatol, 99(4), pp.408~ (1992)
- Paul WE, "Interleukin-4 : a prototypic immunoregulatory lymphokine", Blood, 77, pp.1859~1870 (1991)
- Afkarian M, "T-bet is a STAT1-induced regulator of IL-12R expression in naive CD4+ T cells", Nat Immunol, 3, pp.549~557 (2002)
- Masakatsu Yamashita, "Essential role of GATA3 for the maintenance of Th2 cytokine production and chromatin remodeling at the Th2 cytokine gene loci", The Journal of Biological Chemistry, 279(26), pp.26983~26990 (2004)
- Gavett, S. H, "Interleukin 12 inhibits antigen-induced airway hyperresponsiveness, inflammation, and Th2 cytokine expression in mice", J. Exp. Med., 182, pp.1527~1536 (1995)
- Zheng W, "The transcription factor GATA-3 is necessary and sufficient for Th2 cytokine gene expression in CD4 T cells", Cell, 89, pp.587~596 (1997)
- Ramsey CD, "The hygiene hypothesis and asthma", Curr Opin Pulm Med, 11, pp.14~20 (2005)
- Shirakawa T, "The inverse association between tuberculin responses and atopic disorder", Science, 275, pp.77~79 (1997)
- Finotto S, "Development of spontaneous airway changes consistent with human asthma in mice lacking T-bet", Science, 295, pp.336~338 (2002)
- 고백화, "실용중의외과학", pp.461~464 (1885), 상해과학기술출판사
- 上海中醫學院, "中醫兒科臨床手冊", pp.203~204 (1980), 上海科學技術出版社
- 채병윤, "피부과의 한방치료", 일중사, pp.68~71 (1991)
- 김덕곤, "동의 소아과학", pp.660~664 (2002), 정담
- H Matsuda, "Development of atopic dermatitis-like skin lesion with IgE hyperproduction in NC", Nga mice, 1997, pp.9, 461~466
- Schreiber SL, "The mechanism of action of cyclosporin A and CsA", Immunol Today, 13(4), pp.136~142 (1992)
- Lillehoj HS, "Differntial effect of cyclosporin A on the expression of T and B lymphocyte activation antigen", J Immunol, 133, pp.244~250 (1986)
- Biren CA, "Dermatologic application of cycloporin", Arch Dermatol, 122, pp.1028~1032 (1986)
- Siegel RM, "Inhibition of T cell development in thymic organ culture: implication for the mecahnism of action of cyclosporin A", Eur J Immunol, 147, pp.391~397 (1991)
- Elias J, "Studies of the cellular infiltrate of chronic idiopanic urticaria: prominence of T-lymphocytes, monocytes, and mast cells", J allergy Clin Immunol, 78, pp.914~918 (1986)
- 김병수, "만성 특발성 두드러기 환자에서 저용량 Cyclosporine 치료 효과에 관한 연구", 대한피부과학회지, 45(7), pp.680~686 (2007), 대한피부과학회
- Takano, N, "A method to induce stable atopic dermatitis- like symptoms in NC", Nga mice housed with skin-lesioned mice, 2006, pp.154, 426~430
- Naohiro Watanabe, "Th2 Immune Responses in GATA-3-Transgenic Mice Infected with Heligmosomoides polygyrus", Int Arch Allergy Immunol, 131, pp.11~14 (2003)
