가미보중익기탕과 가미좌귀음의 폐 섬유화 치료 기전에 대한 예비 연구
The Preliminary Study for Therapeutic Mechanism of Gami-Bojungikgitang and Gami-Jwagwieum for Pulmonary Fibrosis
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Objectives In this study, we evaluated the therapeutic effects of Gami-Bojungikgitang and Gami-Jwagwieum for bleomycin-induced lung fibrosis in mice. Methods Extracted lyophilization, Gami-Bojungikgitang (96g) and Gami-Jwagwieum (118g) boiled, filtered, depressed, concentrated, and are obtained. They were divided into five groups: normal, group IA; Animal group treated with bleomycin observed on the 21th day, group IB; Animal group treated with bleomycin observed on the 42th day, group IIA; Animal group treated with bleomycin and Gami-Bojungikgitang. Gami-Jwagwieum observed on the 21th day, group IIB; Animal group treated with bleomycin and Gami-Bojungikgitang/Gami-Jwagwieum observed on the 42th day. Mice are used on the 42th day and as a result, bronchoalveolar lavages fluid is obtained. Counting total number of cells, different ratio of macrophage, lymphocyte, and neutrophil are established. Results In animal group treated with bleomycin and Gami-Bojungikgitang, total cell count decreased by 50% in 3 weeks compared to animal group with non-administrated Gami-Bojungikgitang. However, total cell count in 6 weeks increased compared to 3 weeks although total cell count still decreased compared to animal group with non-administrated Gami-Bojungikgitang. In the view of differential cell counts in bronchoalveolar lavages fluid in treatment group on 3 and 6 weeks, neutrophile was a few and lymphocyte decreased. In animal group treated with bleomycin and Gami-Jwagwieum, total cell count decreased by 50% in 3 and 6 weeks compared to animal group with non-administrated Gami-Jwagwieum. In the view of differential cell counts in bronchoalveolar lavages fluid in treatment group on 3 and 6 weeks, lymphocyte also decreased. Conclusions Gami-Bojungikgitang and especially Gami-Jwagwieum for bleomycin-induced lung fibrosis in mice were effective in total cell count and differential cell count.
Abstract
Objectives In this study, we evaluated the therapeutic effects of Gami-Bojungikgitang and Gami-Jwagwieum for bleomycin-induced lung fibrosis in mice. Methods Extracted lyophilization, Gami-Bojungikgitang (96g) and Gami-Jwagwieum (118g) boiled, filtered, depressed, concentrated, and are obtained. They were divided into five groups: normal, group IA; Animal group treated with bleomycin observed on the 21th day, group IB; Animal group treated with bleomycin observed on the 42th day, group IIA; Animal group treated with bleomycin and Gami-Bojungikgitang. Gami-Jwagwieum observed on the 21th day, group IIB; Animal group treated with bleomycin and Gami-Bojungikgitang/Gami-Jwagwieum observed on the 42th day. Mice are used on the 42th day and as a result, bronchoalveolar lavages fluid is obtained. Counting total number of cells, different ratio of macrophage, lymphocyte, and neutrophil are established. Results In animal group treated with bleomycin and Gami-Bojungikgitang, total cell count decreased by 50% in 3 weeks compared to animal group with non-administrated Gami-Bojungikgitang. However, total cell count in 6 weeks increased compared to 3 weeks although total cell count still decreased compared to animal group with non-administrated Gami-Bojungikgitang. In the view of differential cell counts in bronchoalveolar lavages fluid in treatment group on 3 and 6 weeks, neutrophile was a few and lymphocyte decreased. In animal group treated with bleomycin and Gami-Jwagwieum, total cell count decreased by 50% in 3 and 6 weeks compared to animal group with non-administrated Gami-Jwagwieum. In the view of differential cell counts in bronchoalveolar lavages fluid in treatment group on 3 and 6 weeks, lymphocyte also decreased. Conclusions Gami-Bojungikgitang and especially Gami-Jwagwieum for bleomycin-induced lung fibrosis in mice were effective in total cell count and differential cell count.
참고문헌 (21)
- Adamson IY, "The pathogenesis of bleomycin-induced pulmonary fibrosis in mice", Am J Pathol, 77, pp.185~197 (1974)
- Jones AW, "Bleomycin lung damage: the pathology and nature of the lesion", Br J Dis Chest, 72, pp.321~326 (1978)
- Smith MR, "Curcumin inhibits fibrosis-related effects in IPF fibroblasts and in mice following bleomycin-induced lung injury", Am J Physiol Lung Cell Mol Physiol, 8 (2010)
- Tanaka K, "Therapeutic effect of lecithinized superoxide dismutase on bleomycin-induced pulmonary fibrosis", Am J Physiol Lung Cell Mol Physiol, 298, pp.L348~360 (2010)
- Shimaoka Y, "Effects of IS-741, a synthetic anti-inflammatory agent, on bleomycin-induced lung injury in mice", Lung, 187, pp.331~339 (2009)
- 李東垣, "東垣十種醫書", pp.48~51 (1980), 대성문화사
- 박지수, "보중익기탕가미가 새끼생쥐의 면역능에 미치는 영향", 대한한방소아과학회지, 15(2), pp.15~30 (2001)
- 장개빈, "경악전서", 동양종합통신교육원, 979~1073 (1978)
- 박영주, "가미좌귀음의 알레르기성 기관지천식 반응 조절 효과", 대한한방소아과학회지, 18(2), pp.31~48 (2004)
- 이재성, "金銀花가 Bleomycin에 의한 肺纖維化에 미치는 影響", 경희대학교 대학원, ~ (2004)
- 류한천, "熟地黃이 Bleomycin에 의한 肺纖維化에 미치는 影響", 경희대학교 대학원, ~ (2004)
- 이경희, "黃芪가 C57BL", 6J 생쥐의 Bleomycin유발 肺纖維化에 미치는 影響, 2004
- 이병삼, "麥門冬이 C57BL", 6J 생쥐의 Bleomycin유발 肺纖維化에 미치는 影響, 2004
- 김현지, "前胡가 Bleomycin에 의한 폐 섬유화에 미치는 영향", 대한한방소아과학회지, 22(2), pp.37~49 (2008), 대한한방소아과학회
- 武維屛, "中西醫臨床呼吸病學", pp.217~219 (1998), 中國中醫藥出版社
- 張天嵩, "補氣通肺湯治療特發性肺纖維化12例", 浙江中醫雜誌, 34(2), pp.54~55 (1999)
- 崔紅生, "肺間質纖維化從絡病辨治探析", Journal of Traditional Chinese Medicine, 44(12), pp.946~947 (2003)
- 어수택, "폐 섬유화증의 병인 및 병태 생리", Tuberculosis and Respiratory Diseases, 54(2), pp.167~177 (2003), 대한결핵및호흡기학회
- 陳永辉, "傷見呼吸係疾患中西醫結合診斷與治療", pp.243~251 (1998), 中國醫藥科技出版社
- 성기원, "六味地黃湯 및 補中益氣湯이 Rat의 피부섬유아세포, 사구체메산지움세포 및 혈관내피세포의 老化 遲延에 미치는 影響", 대한한방내과학회지, 24(3), pp.551~560 (2003), 대한한방내과학회
- 신민교, "임상본초학", pp.458~459 (2004), 영림사