치자감초시탕 추출물의 Endocannabinoid system 조절을 통한 아토피피부염 염증조절 효과
Anti-inflammatory Effect of Chijagamchosi-tang Extract through Endocannabinoid system Control in Atopic Dermatitis
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Objective This study aimed to confirm the effect of Chijagamchosi-tang extract on skin damage recovery and inflammation relief in atopic dermatitis-induced mice through Endocannabinoid system (ECS) control. Methods In this study, we used 4-week-old NC/Nga mice which were divided into four groups: control, atopic dermatitis elicitated (ADEG), palmitoylethanolamide (PEA) treated after atopic dermatitis elicitation (PETG), and Chijagamchosi-tang extract treatment groups after atopic dermatitis elicitation (3GTG). Each group was assigned 10 animals. After drug administration for 3 weeks following lipid barrier elimination, cannabinoid receptor (CBR) 1, CBR 2, 8-hydroxydeoxyguanosine (8-OXdG), cluster of differentiation (CD) 68, matrix metalloproteinase (MMP)-9, Fc ε receptor and substance P were observed to confirm the regulation of the ECS, macrophage and mast cell activities. Results We found that CBR1, CBR2, and GPR55 showed higher positive reactions in the 3GTG group than in the ADEG and PETG. 8-OHdG, CD68, and MMP-9 positive reactions were significantly lower in the 3GTG group than in ADEG and PETG groups. Both the Fc ε receptor and substance P showed lower positive reactions in the 3GTG compared to the ADEG and PETG. Conclusion It was confirmed that the Chijagamchosi-tang extract can reduce the inflammation of atopic dermatitis by restoring the structural damage of the skin lipid barrier through ECS activity.
Abstract
Objective This study aimed to confirm the effect of Chijagamchosi-tang extract on skin damage recovery and inflammation relief in atopic dermatitis-induced mice through Endocannabinoid system (ECS) control. Methods In this study, we used 4-week-old NC/Nga mice which were divided into four groups: control, atopic dermatitis elicitated (ADEG), palmitoylethanolamide (PEA) treated after atopic dermatitis elicitation (PETG), and Chijagamchosi-tang extract treatment groups after atopic dermatitis elicitation (3GTG). Each group was assigned 10 animals. After drug administration for 3 weeks following lipid barrier elimination, cannabinoid receptor (CBR) 1, CBR 2, 8-hydroxydeoxyguanosine (8-OXdG), cluster of differentiation (CD) 68, matrix metalloproteinase (MMP)-9, Fc ε receptor and substance P were observed to confirm the regulation of the ECS, macrophage and mast cell activities. Results We found that CBR1, CBR2, and GPR55 showed higher positive reactions in the 3GTG group than in the ADEG and PETG. 8-OHdG, CD68, and MMP-9 positive reactions were significantly lower in the 3GTG group than in ADEG and PETG groups. Both the Fc ε receptor and substance P showed lower positive reactions in the 3GTG compared to the ADEG and PETG. Conclusion It was confirmed that the Chijagamchosi-tang extract can reduce the inflammation of atopic dermatitis by restoring the structural damage of the skin lipid barrier through ECS activity.
참고문헌 (38)
- Kim YJ, "The effect of retinoic acid on expression of human beta defensin-2and LL-37 in keratinocyte", J Korean Invest Dermatol, 14, pp.29~35 (2007)
- Kwon MS, "The effect on the skin barrier function of ceramide", Kor J Aesthet Cosmetol, 3(1), pp.131~137 (2005)
- Yoon YM, "Effects of the complex containing Centella asiatica-and folic acid-fermented extracts, acetyl glutamine, and nicotinic acid adenine dinucleotide phosphate on the inhibition of senescence and elanogenesis, promotion of collagen expression, cellular regeneration, and keratinocyte differentiation, and anti-inflammation", Kor J Aesthet Cosmetol, 11(4), pp.675~684 (2013)
- Steinert PM, "The proteins elafin, filaggrin, keratin intermediate filaments, loricrin, and small proline-rich proteins 1 and 2 are isodipeptide cross-linked components of the human epidermal cornified cell envelope", J Biol Chem, 270(30), pp.17702~17711 (1995)
- Steven AC, "Protein composition of cornified cell envelopes of epidermal keratinocytes", J Cell Sci, 107(2), pp.693~700 (1994)
- Elias PM, "Structural and lipid biochemical correlates of the epidermal permeability barrier", Adv Lipid Res, 24, pp.1~26 (1991)
- Weidinger S, "Atopic dermatitis", Lancet(London, England), 387(10023), pp.1109~1122 (2016)
- Kim JH, "Current understanding of atopic dermatitis", Pediatr Allergy Respir Dis, 14(1), pp.12~23 (2004)
- Im GM, "Oriental medical approach on the allergic disease", J Physiol &Pathol Korean Med, 16(5), pp.831~839 (2002)
- Cha HY, "Hataedock treatment has preventive therapeutic effects for atopic dermatitis through skin barrier protection in Dermatophagoides farinae-induced NC", Nga mice, 2017, pp.206, 327~336
- Muluye RA, "Anti-inflammatory and antimicrobial effects of heat-clearing Chinese herbs : a current review", J Tradit Complement Med, 4(2), pp.93~98 (2014)
- National Korean Medical University Common Textbook Compilation Committee, "Herbology", pp.208~ (2006), Yeongnimsa
- Zhang T, "Therapeutic effects of a fermented soy product on peanut hypersensitivity is associated with modulation of T-helper type 1 and T-helper type 2 responses", Clin Exp Allergy, 38(11), pp.1808~1818 (2008)
- Shan M, "A review on the phytochemistry, pharmacology, pharmacokinetics and toxicology of geniposide, a natural product", Molecules, 22(10), pp.1689~ (2017)
- Fereshteh NK, "Cellular and molecular mechanisms of genistein in prevention and treatment of diseases : An overview", J Food Biochem, 45(11), pp.e13972~ (2021)
- Aum SH, "The anti-inflammatory effects of hataedock taken douchi extracts on atopic dermatitis-like skin lesion of NC", Nga mouse, 2016, pp.30(2), 01~09
- Ahn SH, "The effect of douchi hataedock treatment for dermatophagoides farinae-induced atopic dermatitis-like skin lesions by controlling IL-4 activity", J Pediatr Korean Med, 31(1), pp.43~51 (2017)
- Kim HJ, "Comparative study on the antimicrobial activity of Glycyrrhiza uralensis and Glycyrrhiza glabra extracts with various countries of origin as natural antiseptics", Korean J Microbiol Biotechnol, 41(3), pp.358~366 (2013)
- Kim HJ, "Antimicrobial activity, quantification and bactericidal activities of licorice active ingredients", Korean J Microbiol Biotechnol, 42(4), pp.386~392 (2014)
- Salzet M, "Invertebrate molecular neuroimmune processes", Brain Res Rev, 34(1), pp.69~79 (2000)
- Yang H, "GPR55-a putative"type 3"cannabinoid receptor in inflammation", J Basic Clin Physiol Pharmacol, 27(3), pp.297~302 (2016)
- Miller LK, "The highs and lows of cannabinoid receptor expression in disease : mechanisms and their therapeutic implications", Pharmacol Rev, 63(3), pp.461~470 (2011)
- Pacher P, "The endocannabinoid system as an emerging target of pharmacotherapy", Pharmacol Rev, 58(3), pp.389~462 (2006)
- Kupczyk P, "Cannabinoid system in the skin-a possible target for future therapies in dermatology", Exp Dermatol, 18(8), pp.669~679 (2009)
- Luger TA, "Neuromediators--a crucial component of the skin immune system", J Dermatol Sci, 30(2), pp.87~93 (2002)
- Maccarrone M, "The endocannabinoid system in human keratinocytes : evidence that anandamide inhibits epidermal differentiation through CB1receptor-dependent inhibition of protein kinase C, activation protein-1, and transglutaminase", J Biol Chem, 278(36), pp.33896~33903 (2003)
- Karsak M, "Attenuation of allergic contact dermatitis through the endocannabinoid system", Science, 316(5830), pp.1494~1497 (2007)
- Casanova ML, "Inhibition of skin tumor growth and angiogenesis in vivo by activation of cannabinoid receptors", J Clin Invest, 111(1), pp.43~50 (2003)
- Piazza PV, "Integrated physiology and pathophysiology of CB1-mediated effects of the endocannabinoid system", Diabetes Metab, 33(2), pp.97~107 (2007)
- Walker JM, "Cannabinoid mechanisms of pain suppression", Handb Exp Pharmacol, 168, pp.509~554 (2005)
- Jhaveri MD, "Endocannabinoid metabolism and uptake : novel targets for neuropathic and inflammatory pain", Br J Pharmacol, 152(5), pp.624~632 (2007)
- Tóth KF, "Cannabinoid signaling in the skin : therapeutic potential of the"c(ut)annabinoid"system", Molecules, 24(5), pp.918~ (2019)
- Amin K, "The role of mast cells in allergic inflammation", Respir Med, 106(1), pp.9~14 (2012)
- Woessner JF Jr, "MMPs and TIMPs-an historical perspective", Mol Biotechnol, 22(1), pp.33~49 (2002)
- Burrage PS, "Matrix metalloproteinases : role in arthritis", Front Biosci, 11(1), pp.529~543 (2006)
- Onodera S, "Macrophage migration inhibitory factor up-regulates expression of matrix metalloproteinases in synovial fibroblast of rheumatoid arthritis", J Biol Chem, 275(1), pp.444~450 (2000)
- Pasparakis M, "Mechanisms regulating skin immunity and inflammation", Nat Rev Immunol, 14(5), pp.289~301 (2014)
- Kay AB, "Allergy and allergic diseases. First of two parts", N Engl J Med, 344(1), pp.30~37 (2001)
