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백호탕추출물의 아토피피부염 유발 백서에서의 피부상피 미세환경 손상 회복

Recovery of Skin Epithelial Microenvironment Damage by Baekho-tang Extract in Atopic Dermatitis-Induced Mice

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Objectives This study aimed to identify the recovery effect of Baekho-tang extract on skin epithelial microenvironment damage in atopic dermatitis-induced mice. Methods In this study, we used 4-week-old NC/Nga mice divided into four groups: lipid barrier elimination group (LBEG), dexamethasone-treated group after lipid barrier elimination (DEXG), Baekho-tang extract treatment group after lipid barrier elimination (BHTG) and control group (Ctrl). Each group was assigned 10 animals. We identified ceramide kinase, casepase 14, sodium hydrogen exchanger, cathelicidine, claudiin, and toll-like receptor 2 through our immunohistochemistry. Results We discovered that when compared to DEXG, ceramide kinase and caspase 14, which are markers of skin moisturizing factors were increased in BHTG. In the antimicrobial barrier-related markers sodium hydrogen exchanger and cathelicidine, as well as the tight junction markers claudin and toll-like receptor 2, the BHTG showed a significant increase compared to the other groups. Conclusions These results suggest that the Baekho-tang extract has a positive effect on strengthening the skin microenvironment by increasing the expression of several indicators of skin moisturizing factors, antimicrobial barriers, and tight junction function.

Abstract

Objectives This study aimed to identify the recovery effect of Baekho-tang extract on skin epithelial microenvironment damage in atopic dermatitis-induced mice. Methods In this study, we used 4-week-old NC/Nga mice divided into four groups: lipid barrier elimination group (LBEG), dexamethasone-treated group after lipid barrier elimination (DEXG), Baekho-tang extract treatment group after lipid barrier elimination (BHTG) and control group (Ctrl). Each group was assigned 10 animals. We identified ceramide kinase, casepase 14, sodium hydrogen exchanger, cathelicidine, claudiin, and toll-like receptor 2 through our immunohistochemistry. Results We discovered that when compared to DEXG, ceramide kinase and caspase 14, which are markers of skin moisturizing factors were increased in BHTG. In the antimicrobial barrier-related markers sodium hydrogen exchanger and cathelicidine, as well as the tight junction markers claudin and toll-like receptor 2, the BHTG showed a significant increase compared to the other groups. Conclusions These results suggest that the Baekho-tang extract has a positive effect on strengthening the skin microenvironment by increasing the expression of several indicators of skin moisturizing factors, antimicrobial barriers, and tight junction function.

참고문헌 (38)

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