아토피피부염에서 갈근과 두시 복합추출물의 Endocannabinoid system 조절을 통한 염증조절

Abstract

Objective This study aimed to confirm the effect of Puerariae Radix and Douchi complex extracts on skin damage recovery and inflammation relief in atopic dermatitis-induced mice through endocannabinoid system (ECS) control. Methods In this study, 4-week-old NC/Nga mice were divided into four groups: control, atopic dermatitis elicited (ADE), palmitoylethanolamide (PEA)-treated after atopic dermatitis elicitation (PEAA), and Puerariae Radix and Douchi complex extract-treated after atopic dermatitis elicitation (PGEA). Ten rats were assigned to each treatment group. After drug administration for 3 weeks following lipid barrier elimination, cannabinoid receptor (CBR) 1, CBR 2, 8-hydroxydeoxyguanosine (8-OHdG), cluster of differentiation (CD) 68, matrix metalloproteinase (MMP)-9, Fc ε receptor, and substance P were observed to confirm the regulation of the ECS, macrophage, and mast cell activities. Results The PGEA group showed more positive reactions for CBR1, CBR2, and GPR55 than the ADE and PEAA groups. The number of positive reactions to 8-OHdG, CD68, and MMP-9 was significantly lower in the PGEA group than in the ADE and PEAA groups. Both the Fc ε receptor and substance P showed lower positive reactions in the PGEA group than in the ADE and PEAA groups. Conclusion The Puerariae Radix and Douchi complex extract can reduce the inflammation of atopic dermatitis by restoring the structural damage of the skin lipid barrier through ECS activity.