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SH-SY5Y 인간 신경모세포종 세포에서 MPTP 유발 세포 독성에 대한 거저리(Tenebrio molitor) 추출물의 보호효과

Protective effects of mealworm (Tenebrio molitor) extract on N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)–induced cellular toxicity in SH-SY5Y neuroblastoma cells

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Purpose: Edible insect extracts have been used as an alternative source for medicinal supplements due totheir significant antioxidative and anti-inflammatory activity. Recent studies have reported that anti-mi crobial peptides from insects have neuroprotective effects on dopamine toxins. The purpose of this studywas to investigate the protective functions of mealworm (Tenebrio molitor) extract (MWE) on N-meth yl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)–induced cellular toxicity in SH-SY5Y neuroblastoma cells. Methods: Cellular toxicity induced by the MPTP toxin and the impact of MWE on cell survival were ana lyzed using MTT assays. DAPI staining was performed to observe apoptotic phenomena caused by MPTP. Changes in caspase-3 activity and protein expression were observed using enzyme activity assays andwestern blot assays, respectively. Results: MWE exerted significant antioxidant activity, which was measured by both DPPH and ABTS radi cal assays, with a dose-dependent relationship. Furthermore, MWE resulted in cellular proliferation in SH SY5Y cells in a dose-dependent manner. Furthermore, MWE pretreatment significantly inhibitedMPTP-induced cytotoxicity, with a dose-dependent relationship. The morphological characteristics ofapoptosis and increased reactive oxygen species induced by MPTP were also significantly reduced byMWE pretreatment. Conclusion: MWE treatment significantly attenuated MPTP-induced changes in the levels of proteins asso ciated with apoptosis, such as caspase-3 and PARP. These findings suggest that MWE exerts neuroprotectiveeffects on human neuroblastoma SH-SY5Y cells subject to MPTP-induced dopaminergic neurodegeneration.

Abstract

Purpose: Edible insect extracts have been used as an alternative source for medicinal supplements due totheir significant antioxidative and anti-inflammatory activity. Recent studies have reported that anti-mi crobial peptides from insects have neuroprotective effects on dopamine toxins. The purpose of this studywas to investigate the protective functions of mealworm (Tenebrio molitor) extract (MWE) on N-meth yl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)–induced cellular toxicity in SH-SY5Y neuroblastoma cells. Methods: Cellular toxicity induced by the MPTP toxin and the impact of MWE on cell survival were ana lyzed using MTT assays. DAPI staining was performed to observe apoptotic phenomena caused by MPTP. Changes in caspase-3 activity and protein expression were observed using enzyme activity assays andwestern blot assays, respectively. Results: MWE exerted significant antioxidant activity, which was measured by both DPPH and ABTS radi cal assays, with a dose-dependent relationship. Furthermore, MWE resulted in cellular proliferation in SH SY5Y cells in a dose-dependent manner. Furthermore, MWE pretreatment significantly inhibitedMPTP-induced cytotoxicity, with a dose-dependent relationship. The morphological characteristics ofapoptosis and increased reactive oxygen species induced by MPTP were also significantly reduced byMWE pretreatment. Conclusion: MWE treatment significantly attenuated MPTP-induced changes in the levels of proteins asso ciated with apoptosis, such as caspase-3 and PARP. These findings suggest that MWE exerts neuroprotectiveeffects on human neuroblastoma SH-SY5Y cells subject to MPTP-induced dopaminergic neurodegeneration.

참고문헌 (30)

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