규칙적인 수영 훈련이 L-NANE 유발 고혈압 흰쥐 좌심실의 제Ⅰ형 Collagen 분포와 좌심실 비대에 미치는 영향
Effect of regular swimming training on left ventricular type Ⅰcollagen volume & Left ventricular hypertrophy in L-NAME induced hypertensive rats.
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이 연구의 목적은 Nitric Oxide 합성억제로 유도된 고혈압 흰쥐에 있어서 규칙적인 수영 훈련이 좌심실의 제1형 Collagen 섬유 분포에 미치는 영향을 파악하여 항고혈압 및 좌심실의 병적 비대를 막는 효과를 알아보는 것이다. 연구 대상은 Sprague- Dawley Rat(n=40)을 통제집단(Con, n=10), 고혈압 통제집단(L-Con, n=10), 고혈압 운동집단(L-Ex, n=10), 수영 훈련집단(Ex, n=10) 등 총 4집단으로 분류하고, 고혈압은 Nω-nitro-L-argine methyl ester(L-NANE, 50 mg․kg-1 day-1)를 복강 내 주사하여 유발시켰다. 수영 훈련 기간은 8주동안 주당 5일, 하루에 30분씩 실시하였다. 몸무게, 수축기 혈압, 심박수는 실험 기간동안 매주 1회 측정 하였으며, 8주 후에 희생시켜 심장을 적출하여 좌심실의 제Ⅰ형 Collagen 분포와 좌심실 비대를 분석하였다. 연구 결과 수축기 혈압과 심박수는 고혈압 운동 집단에서 유의하게 떨어졌고(p<.001), 좌심실의 제Ⅰ형 Collagen 분포는 고혈압 통제집단이 다른 집단에 비해 유의하게 높았으며(p<.001), 고혈압 운동집단에서는 유의한 차는 없었지만 통제집단 보다 낮은 분포를 나타내었다. 좌심실 벽 두께도 고혈압 통제집단이 모든 집단에 비해 유의하게 두꺼웠으며(p<.001), 운동집단은 통제집단 보다 좌심실 벽 두께가 유의하게 얇았다(p<.05). 또한, 고혈압 운동집단은 유의한 차는 없었지만 통제군 보다 얇았다. 결론적으로 8주간의 수영 훈련은 L-NANE 유발 고혈압 흰쥐에 혈관 확장과 제Ⅰ형 Collagen 섬유 생성을 줄임으로써 좌심실 비대를 막는 효과를 알 수 있었다. 이것으로 수영훈련은 항고혈압 및 심근 기능 향상에 효과가 있음을 알 수 있었다.
Abstract
The aim of this study was to investigate the antihypertensive effect of swimming training assessing left ventricular type Ⅰcollagen volume & Left ventricular hypertrophy in nitric oxide synthase inhibitor-induced hypertensive rats. This study forty Sprague-Dawley rats aged 8weeks old were assigned to control group (Con: n=10), control+L-NAME group (L-Con: n=10), L-NAME+swimming group(L-Ex: n=10) and swimming group (Ex n=10). Hypertension was induced by injection (I.P) of, Nω-nitro-L-arginine methyl ester (L-NAME, 50 mg․kg-1day-1). Animals in swimming groups were subjected to swimming training, 5 day/week with duration of 30 minutes for 8 weeks. Blood pressure, body weight and heart rate were monitored during the session experimental period. The animals were sacrificed at the last day of the 8 weeks thickness of the left ventricular wall and left ventricular type Ⅰcollagen volume were measured. The results of this study showed that induced in heart rate and blood pressure decreased significantly in the L-Exe group compared to the other groups (p＜.001). Compared to the other groups, the L-Con group was significantly increased Left ventricular type Ⅰcollagen volume (p＜.001). The thickness of the left ventricular wall was decreased in the L-Ex and the Ex groups than in the L-Con group (p＜.001) and the Con groups (p＜.05). The current finding of the suggest that regular swimming have vasodilatory capacity of the artery and decreased type Ⅰ collagen volume, resulting in prevented left ventricular hypertrophy in L-NAME induced hypertensive rats. In conclusion, swimming training effects by antihypertensive and increased myocardium function.
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