파프리카에 발생하는 주요 병원균에 대한 길항미생물, Serratia marcescens-YJK1, 분리와 특성
The Isolation and Characterization of the Antagonistic Microorganisms, Serratia marcescens-YJK1, for Major Pathogens on Paprika
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Synthetic agro-chemicals have been widely used to control diseases on paprika but these days negative attention has been increasing to use of them because of several adverse effects. This research was conducted to isolate and to characterize the antagonistic microorganism to control major paprika diseases, gray mold rot, fruit and stem rot, phytophthora blight, sclerotium rot, and wilt disease. Analysis of the fatty acid and analysis of the 16S rDNA gene sequence revealed that YKJ1 isolated in this research belongs to a group of Serratia marcescens. Specially, 16S rDNA gene sequence of YKJ1 showed 99% of sequence similarity with S. marcescens. Observation through the optical microscope revealed that YKJ1 suppressed the spore germination and the hyphal growth of pathogens. YKJ1 treatment on pathogens induced marked morphological changes like hyphal swelling and degradation of cell wall. In the case of phytophthora blight, the zoosporangium formation was restrained. S. marcescens found in this study call as S. marcescens-YKJ1 and it may be valuable as one of biological control agents against major diseases of paprika in the future even though it is require to be tested with more study on field test
Abstract
Synthetic agro-chemicals have been widely used to control diseases on paprika but these days negative attention has been increasing to use of them because of several adverse effects. This research was conducted to isolate and to characterize the antagonistic microorganism to control major paprika diseases, gray mold rot, fruit and stem rot, phytophthora blight, sclerotium rot, and wilt disease. Analysis of the fatty acid and analysis of the 16S rDNA gene sequence revealed that YKJ1 isolated in this research belongs to a group of Serratia marcescens. Specially, 16S rDNA gene sequence of YKJ1 showed 99% of sequence similarity with S. marcescens. Observation through the optical microscope revealed that YKJ1 suppressed the spore germination and the hyphal growth of pathogens. YKJ1 treatment on pathogens induced marked morphological changes like hyphal swelling and degradation of cell wall. In the case of phytophthora blight, the zoosporangium formation was restrained. S. marcescens found in this study call as S. marcescens-YKJ1 and it may be valuable as one of biological control agents against major diseases of paprika in the future even though it is require to be tested with more study on field test
참고문헌 (37)
- Adhikari, T. B., "Evaluation of bacteria isolated from rice for plant growth promotion and biological control of seeding disease of rice", Can. J. Microbiol, 47, pp.916~924 (2001)
- Cha, S. D., "Characterization of fungal pathogens isolated from greenhouse grown paprika plants and imported paprika seeds", 석사, pp.2009
- Cho, D. C., "Pattern of Phytophthora blight occurrence in paprika grown in hydroponic system and application of sodium hypochlorite for its control", 석사, pp.2010
- Chung, Y. S., "Characteristics of gray mold on paprika (Capsicum annuum) caused by Botrytis cinerea and evaluation of biological control agent", 석사, pp.2011
- Felsenstein, J., "Confidence limits on phylogenies: An approach using the bootstrap", Evolution, 39, pp.783~791 (1985)
- Gunji, S., "Screening of antifungal antibiotics according to activities inducing morphological abnormalities", Agric. Biol. Chem, 47, pp.2061~2069 (1983)
- 지형진, "Occurrence of Stem and Fruit Rot of Paprika Caused by Nectria haematococca", The Plant Pathology Journal, 21(4), pp.317~321 (2005), 한국식물병리학회
- 지형진, "Nectria haematococca에 의한 착색단고추 줄기 및 과실썩음병의 약제방제", 식물병 연구, 11(2), pp.179~184 (2005), 한국식물병리학회
- 김성환, "Characterization of Sclerotinia sclerotiorum Isolated from Paprika", Mycobiology, 34(3), pp.154~157 (2006), 한국균학회
- 김재현, "Characterization of Cucumber mosaic virus Subgroup II Isolated from Parkika(Capsicum annuum var.grossum)in Korea", The Plant Pathology Journal, 18(1), pp.6~11 (2002), 한국식물병리학회
- Kim, K. Y., "Biological control of Pyricularia oryzae blast spot with the antibiotic substances produced by Bacillus sp. KL-3", Kor. J. Appl. Microbiol. Biotechnol, 25, pp.396~402 (1997)
- Kim, Y. K, "Biological control of phytophthora blight of Redpepper by antagonistic bacillus polymyxa ‘AC-1’", 박사, pp.1995
- Kim, Y. K., "Trend of environment friendly agro material development on the plants of solanaceae (Pepper, Eggplant, Tomato)", KIC News, 14(4), pp.19~28 (2011)
- Kimura, M., "A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences", J. Mol. Evol, 16, pp.111~120 (1980)
- (2013)[URL]
- Lee, E. T., "Selection and antifungal activity of antagonistic bacterium Pseudomonas sp. 2112 against Red-Pepper Rotting Phytophthora capsici", Kor. J. Appl. Microbiol. Biotechnol, 28, pp.334~340 (2000)
- Lee, H. Y, "Isolation and biological control of antagonistic microorganisms for sesame wilt", 석사, pp.1994
- Lee, J. M., "Isolation of siderophore- producing Pseudomonas fluorescens GL7 and its biocontrol activity against root-rot disease for the development", Kor. J. Appl. Microbiol. Biotechnol, 27, pp.427~432 (1999)
- Lee, S. K., "Diversity of rice endophytic bacteria and antagonistic effect", 박사, pp.2004
- Lee, W. H., "Advantages and disadvantages in using biological control of plant diseases and integrated control", J. Agricult. Life Sci, 39, pp.66~76 (2008)
- Li, B., "Characterization and comparison of Serratia marcescens isolated from edible cactus and from silkworm for virulence potential and chitosan susceptibility", Braz. J. Microbiol, 42, pp.96~104 (2011)
- Lim, H. S., "Role of siderphores in biocontrol of Fusarium solani and enhanced growth response of bean by Pseudomonas fluorescens GL20", J. Microbiol. Biotechnol, 7, pp.13~20 (1997)
- Moussa, T. A. A., "Biocontrol of sugarbeet pathogen Fusarium solani (Mart.) Sacc. by Streptomyces aureofaciens", Pakistan J. Biol. Sci, 5, pp.556~559 (2002)
- Mehnaz, S., "Isolation and 16S rRNA sequence analysis of the benefical bacteria from the rhizosphere of rice", Can. J. Microbiol, 47, pp.110~117 (2001)
- 문혜연, "Outbreak of Cucumber mosaic virus and Tomato spotted wilt virus on Bell PepperGrown in Jeonnam Province in Korea", The Plant Pathology Journal, 24(1), pp.93~96 (2008), 한국식물병리학회
- Nam, K. W., "Ecology and management of main diseases of Sweet pepper in hydroponic culture", Kor. Res. Soc. Protected Hort, 16, pp.23~30 (2003)
- Nunes, C., "Biological control of postharvest pear diseases using a bacterium, Pantoea agglomerans CPA-2", Int. J. Food Microbiol, 70, pp.53~61 (2001)
- Osterhout, G. J., "Identification of clinical isolates of gram-nagative non fermentative bacteria by an automated cellular fatty acid identification system", J. Cln. Microbiol, 29, pp.1822~1830 (1991)
- Reysenbach, A. L., "Differential amplification of rDNA genes by polymerase chain reaction", Appl. Environ. Microbiol, 58, pp.3417~3418 (1992)
- Sacchi, C. T., "Sequencing of 16S rRNA gene; A rapid tool for identification of Bacillus anthracis", Emerg. Infect. Dis, 8, pp.1117~1123 (2002)
- Saitou, N., "The neighbor-joining method: A new method for reconstructing phylogenetic trees", Mol. Biol. Evol, 4, pp.406~425 (1987)
- Identification of bacteria by gas chromatography of cellular fatty acids, "Sasser, M.", MIDI, Inc, 1~7, pp.MIDI technical note 101 (1990)
- Seong, K. Y., "Effect of siderophore on biological control of plant pathogens and promotion of plant growth by Pseudomonas fluorescens ps88", Agri. Chem. Biotechnol, 39, pp.20~24 (1996)
- Tamura K., "MEGA4: Molecular volutionary Genetics Analysis (MEGA) software version 4.0", Mol. Biol. Evol, 24, pp.1596~1599 (2007)
- Walker, R., "The potential biocontrol agent Pseudomonas antimicrobica inhibits germination of conidia and outgrowth of Botrytis cinerea", Lett. Appl. Microbiol, 32, pp.346~348 (2001)
- Yang, P., "Application of fatty acid methyl esters for the taxonomic analysis of the genus Xanthomonas", Syst. Appl. Microbiol, 16, pp.47~71 (1993)
- 김병섭, "Survey of Fungicide Resistance for Chemical Control of Botrytis cinerea on Paprika", The Plant Pathology Journal, 24(4), pp.447~452 (2008), 한국식물병리학회
