고추 탄저병 방제제 Burkholderia lata CAB13001선발 및 포장방제 효과
Selection of Antifungal Bacteria Burkholderia lata CAB13001 for Control on Red Pepper Anthracnose andIts Control Efficacy in Field
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초록
To control the pepper anthracnose caused by Colletotrichum acutatum, antifungal bacterium strains which was selected among bacterium from natural soil, was tested the antimicrobial activity against various pathogens and its control efficacy on anthracnose disease in the fields. We confirmed that antagonistic activity of CAB13001 strain to pathogens such as Sclerotinia cepivorum, Sclerotinia sclerotium and Botrytis cinerea including Colletotrichum acutatum was remarkable superior with the dual culture method in the artificial medium. In vitro bioassay using the green pepper fruit, CAB13001 strain suppressed the lesion development of Anthracnose disease, and its control value compared to the untreated one was 82.4% on pepper fruit in field test. These results suggested that CAB13001 strain could be a very useful biological control agents to anthracnose disease caused by air born plant pathogens of pepper. By the way, analysis of nucleotide sequence of the gene 16S rDNA, antagonistic bacterium CAB13001 strain used in this study was identified as Burkholderia lata.
Abstract
To control the pepper anthracnose caused by Colletotrichum acutatum, antifungal bacterium strains which was selected among bacterium from natural soil, was tested the antimicrobial activity against various pathogens and its control efficacy on anthracnose disease in the fields. We confirmed that antagonistic activity of CAB13001 strain to pathogens such as Sclerotinia cepivorum, Sclerotinia sclerotium and Botrytis cinerea including Colletotrichum acutatum was remarkable superior with the dual culture method in the artificial medium. In vitro bioassay using the green pepper fruit, CAB13001 strain suppressed the lesion development of Anthracnose disease, and its control value compared to the untreated one was 82.4% on pepper fruit in field test. These results suggested that CAB13001 strain could be a very useful biological control agents to anthracnose disease caused by air born plant pathogens of pepper. By the way, analysis of nucleotide sequence of the gene 16S rDNA, antagonistic bacterium CAB13001 strain used in this study was identified as Burkholderia lata.
참고문헌 (18)
- Frank, J. A., "Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes", Appl. Environ. Microbiol, 74, pp.2461~2470 (2008)
- 한광섭, "Burkholderia pyrrocinia CAB08106-4 균주를 이용한 마늘 흑색썩음균핵병의 생물학적 방제", 식물병 연구, 19(1), pp.21~24 (2013), 한국식물병리학회[DOI]
- Hildebrand, D. C., "Identification Schemes: Laboratory Guide for Identification of Plant Pathogenic Bacteria", pp.61~ (1988), APS Press
- 강범관, "고추 탄저병균 Colletotrichum acutatum의 포장 밀도 조사를 위한반선택 배지의 확립 및 활용", 식물병 연구, 11(1), pp.21~27 (2005), 한국식물병리학회
- 강훈석, "길항세균 Bacillus sp. KBC1004를 이용한 고추탄저병의 생물학적방제제 개발", 식물병 연구, 21(3), pp.208~214 (2015), 한국식물병리학회[DOI]
- 김병련, "Bacillus toyonensis CAB12243-2 균주를 이용한배추 무름병의 생물적 방제", 한국유기농업학회지, 26(1), pp.129~140 (2018), 한국유기농업학회
- Kim, C. H., "A predictive model of disease progression of red-pepper anthracnose", Plant Pathol. J, 4, pp.325~331 (1988)
- 김성택, "점액세균 Myxococcus sp. KYC 1126을 이용한 고추 역병 생물학적 방제 효능", 식물병 연구, 17(2), pp.121~128 (2011), 한국식물병리학회
- Kumar S., "MEGA X : Molecular Evolutionary Genetics Analysis across computing platforms", Molecular Biology and Evolution, 35, pp.1547~1549 (2018)
- 권천섭, "고추 탄저병의 발병 생태 특성", 식물병 연구, 8(2), pp.120~123 (2002), 한국식물병리학회
- 임태헌, "토양 방선균 Streptomyces sp. MG 121의 항균활성 및 고추 생육에 미치는 효과", 식물병 연구, 13(2), pp.93~97 (2007), 한국식물병리학회
- Narisawa, K., "Suppression of clubroot formation in Chinese cabbage by the root endophytic fungus, Heteroconium chaetospira", Plant Pathol, 47, pp.206~210 (1998)
- Park, K. S., "Identification, distribution and etiological characteristics of anthracnose fungi of red pepper in korea", Plant Pathol. J, 8, pp.61~69 (1992)
- 박세정, "고추 역병과 탄저병에 대한 친환경유기농자재의 방제 효과", 식물병 연구, 18(1), pp.1~9 (2012), 한국식물병리학회[DOI]
- Shin, H. J., "Comparison of pepper anthracnose pathogen from korea and china", Plant Pathol. J, 8, pp.61~69 (1999)
- 소재우, "고추 탄저병 병원균에 따른 친환경자재의 방제 효과", 한국국제농업개발학회지, 26(2), pp.162~168 (2014), 한국국제농업개발학회[DOI]
- List of Plant Disease in Korea, "The Korean Society of Plant Pathology(KSPP)", The Korean Society of Plant Pathology, 106~ (2009)
- 유재홍, "Bacillus sp. NAAS-1을 이용한 고추 탄저병 생물학적 방제", 한국균학회지, 40(4), pp.277~281 (2012), 한국균학회