오배자(Schlechtendalia chinensis)로부터 수박 과실썩음병 병원균(Acidovorax avenae subsp. citrulli)에 대한 항균 활성물질 탐색
Isolation of Antimicrobial Active Substances from Chinese Gall Nut (Schlechtendalia chinensis) against Watermelon Fruit Rot Pathogens (Acidovorax avenae subsp. Citrulli)
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참고문헌
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초록
This study was conducted to develop environment-friendly agricultural products with anti-microbial activity against Acidovorax avenae subsp. citrulli as a pathogen of bacterial fruit blotch in cucurbit. Schlechtendalia chinensis was extracted by MeOH and solvent fraction. The hexane fraction, which showed highest value of anti-microbial activity, was analyzed by GC-MS. Each mass spectra, corresponding to each peak of chromatogram, was compared to MS database of Wiley library. As a result, myristic acid, palmitic acid and 3-n-pentadecylphenol were identified as maine compounds showing antimicrobial activity against A. avenae subsp. citrulli. Bioassay using commercial myristic acid, palmitic acid and 3-n-pentadecylphenol to test for the anti-microbial activity conformed the anti-microbial activity of potential active compounds, and myristic acid and 3-n-pentadecylphenol showed strong activity. In conclusion, myristic acid and 3-n-pentadecylphenol identified from S. chinensis were anti-microbial chemicals.
Abstract
This study was conducted to develop environment-friendly agricultural products with anti-microbial activity against Acidovorax avenae subsp. citrulli as a pathogen of bacterial fruit blotch in cucurbit. Schlechtendalia chinensis was extracted by MeOH and solvent fraction. The hexane fraction, which showed highest value of anti-microbial activity, was analyzed by GC-MS. Each mass spectra, corresponding to each peak of chromatogram, was compared to MS database of Wiley library. As a result, myristic acid, palmitic acid and 3-n-pentadecylphenol were identified as maine compounds showing antimicrobial activity against A. avenae subsp. citrulli. Bioassay using commercial myristic acid, palmitic acid and 3-n-pentadecylphenol to test for the anti-microbial activity conformed the anti-microbial activity of potential active compounds, and myristic acid and 3-n-pentadecylphenol showed strong activity. In conclusion, myristic acid and 3-n-pentadecylphenol identified from S. chinensis were anti-microbial chemicals.
참고문헌 (20)
- Assouline, I, "Acidovorax avenae subsp. citrulli Transmitted by Solanaceous seed", Phytoparasitica, 25, pp.2~3 (1997)
- 차재영, "국내 자생 향장식물 에탄올 추출물의 항균 효과", 생명과학회지, 18(2), pp.228~233 (2008), 한국생명과학회
- 최일, "Staphylococcus aureus와 Salmonella gallinarum에 대한 한약재의 항균활성", 한국미생물·생명공학회지, 30(2), pp.177~183 (2002), 한국미생물·생명공학회
- Hopkins, D. L, "Wet seed treatments for the control of bacterial fruit blotch of watermelon", Plant Dis, 30, pp.529~532 (1996)
- Hopkins, D. L, "Bacterial leaf spot of watermelon caused by Pseudomonas lachrymans", Phytopathol, 62, pp.542~545 (1971)
- Hopkins, D. L, "Seed transmission of Acidovorax avenae subsp. citrulli in cucurbits", HortSci, 37, pp.924~926 (2002)
- Isakeit, T, "First report of infection of honeydew with Acidovorax avenae subsp. citrulli", Plant Dis, 81, pp.694~ (1997)
- Jo, H. C, "Gall formation on different age, habitat, and parasite position in Rhus javanica L", Kor J Med Crop Sci, 8, pp.304~311 (2000)
- Langston, D. B. Jr, "First reportof a fruitrot pumpkin caused by Acidovorax avenae pv. citrulli in Georgia", Plant Dis, 83, pp.199~ (1999)
- Latin, R. X, "Bacterial fruit blotch of watermelon: the hypothetical exam question becomes reality", Plant Dis, 79, pp.761~765 (1995)
- Latin, R. X, "Bacterial fruit blotch of watermelon in Indiana", Plant Dis, 74, pp.331~ (1990)
- Lessl, J. T, "Colonization of female watermelon blossoms by Acidovorax avenae subsp. Citrulli and the relationship between blossom inoculum dosage and seed infestation", J. Phytopathol, 155, pp.114~121 (2007)
- Martin, H. L, "First report of Acidvorax avenae subsp. cirulli as a pathogen of cucumber", Plant Dis, 83, pp.965~ (1999)
- Rane, K. K, "Bacterial fruit blotch of watermelon-association of the pathogen with seed", Plant Dis, 76, pp.509~512 (1992)
- 서상태, "Acidovorax avenae subsp. citrulli에 의한 멜론 과실썩음병", 식물병 연구, 12(3), pp.185~188 (2006), 한국식물병리학회
- Somodi, G. C, "Occurrence of a bacterial watermelon fruit blotch in Florida", Plant Dis, 75, pp.1053~1056 (1991)
- Song, W. Y, "Pseudomonas pseudoalcaligenes subsp. citrulli: The causal agent of bacterial fruit blotch rot on watermelon", Kor. J. Plant Pathol, 7, pp.177~182 (1991)
- Song, W, Y, "A new selsctive medium for detecting Acid Acidovorax avenae subsp. avenae in rice seeds", Plarhol. J, 16, pp.236~241 (2000)
- Sowel, G. Jr, "Pseudomonas pseudoalcaligenes subsp. citrulli on watermelon: Seed transmission and resistance of plant introductions", Plant Dis. Rep, 63, pp.437~441 (1979)
- Wall, G. C, "Outbreak of watermelon fruit blotch in the Mariana islands", Plant Dis, 74, pp.80~ (1990)
