송라(Usnea longissima)추출물로부터 균핵병 병원균(Sclerotinia sclerotiorum)에 대한 항균 활성물질 탐색
Isolation of Antimicrobial Active Substance from Usnea longissima against Sclerotial Rot (Sclerotinia sclerotiorum)
1
조회
0
다운로드
5
인용
0
참고문헌
키워드
초록
To develop environment-friendly agricultural products with anti-microbial activity against Sclerotinia sclerotiorum as a pathogen of sclerotium disease, Usnea longissima was extracted by methanol and its extract was fractionated into several solvent fractions. The chloroform fraction, which showed the highest antimicrobial activity, was separated by silica gel-column chromatography and obtained into nine group subfractions. The nine group fractions were searched the antifungal activities by bioassay. The most active No. 3 subfraction was analyzed by GC-MS. Each mass spectra, corresponding to each peak of chromatogram, was compared to database of Wiley library. As a result, Usnic acid was identified as main compounds. In conclusion, Usnic acid isolated from Usnea longissima was antimicrobial chemical against Sclerotinia sclerotiorum as a pathogen of sclerotium disease.
Abstract
To develop environment-friendly agricultural products with anti-microbial activity against Sclerotinia sclerotiorum as a pathogen of sclerotium disease, Usnea longissima was extracted by methanol and its extract was fractionated into several solvent fractions. The chloroform fraction, which showed the highest antimicrobial activity, was separated by silica gel-column chromatography and obtained into nine group subfractions. The nine group fractions were searched the antifungal activities by bioassay. The most active No. 3 subfraction was analyzed by GC-MS. Each mass spectra, corresponding to each peak of chromatogram, was compared to database of Wiley library. As a result, Usnic acid was identified as main compounds. In conclusion, Usnic acid isolated from Usnea longissima was antimicrobial chemical against Sclerotinia sclerotiorum as a pathogen of sclerotium disease.
참고문헌 (29)
- Abawi, G. S, "Source of primary inoculum and effects of temperature and moisture on infection of beans by Whetzelinia sclerotiorum", Phytopathology, 65, pp.300~309 (1975)
- Abawi, G. S, "Epidemiology of disease caused by Sclerotinia species", Phytopathology, 69, pp.899~904 (1979)
- Agrios, G. N, "Plant pathology", pp.635~ (1998), Academic Press
- Bardin, S. D, "Research on biology and control of Sclerotinia diseases in Canada", Can. J. Plant Pathol, 23, pp.88~98 (2001)
- Baskar K., "Bioefficacy of Aristolochia tagala Cham. against Spodoptera litura Fab. (Lepidoptera: Noctuidae)", Saudi Journal of Biological Sciences, 18, pp.23~27 (2011)
- Boland, G. J, "Index of host plants of Sclerotinia sclerotiorum", Canadian journal of Plant Pathol, 16, pp.93~108 (1994)
- Cho, C. T, "Present Status and Problems for the Control of Vegetable Diseases in the Vinyl house", Korean J. Plant Protection, 154, pp.215~222 (1976)
- Hall, B. H, "First report of Sclerotinia sclerotiorumon grape (Vitis vinifera) in South Australia", Australasian Plant Pathol, 31, pp.417~418 (2002)
- 홍성기, "국내 오디균핵병의 발생 양상", 식물병 연구, 13(3), pp.131~136 (2007), 한국식물병리학회
- 황지영, "상추 균핵병 생물적방제를 위한 Brevibacillus brevis B23과 Bacillus stearothermophilus B42의 선발", 식물병 연구, 12(3), pp.254~259 (2006), 한국식물병리학회
- 강재영, "잔디 병해 방제를 위한 약용식물의 항균작용 탐색", Weed & Turfgrass Science, 2(1), pp.70~75 (2013), 한국잔디학회
- 김은옥, "뽕나무(Morus alba L.) 품종별 오디의 영양 및 기능성 성분과 이화학적 품질 특성 비교", 한국식품영양과학회지, 39(10), pp.1467~1475 (2010), 한국식품영양과학회
- Kim, H. B, "Anti-oxidative capacity analysis of water-soluble substances according to varieties and maturity stages in mulberry leaves and fruits", Kor. J. Seric. Sci, 47(2), pp.62~67 (2005)
- Kim, H. W, "Isolation and identification of antagonistic bacterium active against Sclerotinia sclerotiorum causing sclerotinia rot on crisphead lettuce", Res. Plant Dis, 12, pp.254~259 (2004)
- Kim, K. C, "The effect of ray on sclerotia formation of sclerotium disease", Korean J. Plant Protect, 15, pp.223~243 (1976)
- Kim, W. G, "Occurrence of Sclerotinia rot on cucurbitaceous vegetable crops in greenhouses", Korean J. Mycol, 27, pp.198~205 (1999)
- 김완규, "Occurrence of Sclerotinia Rot in Cruciferous Crops Caused by Sclerotinia spp.", The Plant Pathology Journal, 19(1), pp.69~74 (2003), 한국식물병리학회
- 김완규, "Occurrence of Sclerotinia Rot in Four Leguminous Crops Caused by Sclerotiniasclerotiorum", The Plant Pathology Journal, 22(1), pp.16~20 (2006), 한국식물병리학회
- Kora, C, "Sclerotinia rot of carrot: An example of phenological adaptation and bicyclic development by Sclerotinia sclerotiorum", plant Dis, 87, pp.456~470 (2003)
- The Korean Society of Plant Pathology, "List of Plant Diseases in Korea", 779~ (2004)
- Krȁmer, P, "Rational approach to fraction, isolation and characterization of polysaccharides from the lichen Cetraria islandica", Drug Research, 45, pp.72~731 (1995)
- Latorre, B. A, "First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Chile", Plant Dis, 85, pp.1122~ (2001)
- Purdy, L. H, "Sclerotinia sclerotiorum: History, diseases and symptomatology, host range, geographic distribution, and impact", Phytopathology, 69, pp.875~880 (1979)
- Rural Development Administration, "Culivation techniques of Mulberry", 6~7 (2010)
- Schwartz, H. F, "Factors affecting sclerotium populations of, and apothecium production by Sclerotinia sclerotiorum", phytopathology, 68, pp.383~388 (1978)
- Shin, D. B, "Ecological studies on lettuce drop disease occurring under controlled cultivation conditions in drained paddy fields.", Korean J. Plant Pathol, 3, pp.252~260 (1987)
- Subbarao, K. V, "Progress toward integrated management of lettuce drop", Plant Dis, 82, pp.1068~1078 (1998)
- Thirugnanasampandan R, "Antioxidant properties of some medicinal Aristolochiaceae species", African Journal of Biotechnology, 7(4), pp.357~361
- Whipps, J. M, "A glasshouse cropping method for screening lettuce lines for resistance to Sclerotinia sclerotiorum", Eur. J. Plant Pathol, 108, pp.373~378 (2002)