혼합영양 조건하에서 Haematococcus sp.의 배지 최적화 및 대사산물 생산
Medium Optimization for Cell Growth and Metabolite Formation from Haematococcus sp. under Mixotrophic Cultivation
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초록
Haematococcus sp.를 대상으로 mixotroph 형태에서 배양을 위한 배지 최적화 연구를 수행하였다. 기본 배지로는modified MS 배지가 적절했으며, 탄소원으로는 glucose가, 그리고 농도는 10 g/l가 적합하였다. 질소원으로는 KNO3를선정하였으며, 농도는 1.9 g/l이 최적이었다. 최적의 배지조건에서 Haematococcus sp.를 초기 접종량(0.18 g/l)로 접종하여 14일 후에 5.58 ± 0.25 g/l로 성장하였으며, 이는 건조세포중량 기준으로 약 31배의 성장한 것이다. 이때 생성된클로로필은 172.16 ± 7.79 mg/l였으며, 카로티노이드는42.33 ± 1.91 mg/l이었다. 본 연구의 결과는 추후 미세조류대량 배양과 대사산물의 생산에 이용가능한 기초자료가 될수 있을 것이라 판단된다.
Abstract
In this study, the medium optimization for cell growth and metabolite formation of Haematococcus sp. under mixotrophic cultivation was investigated. As a result, modified MS medium was selected as the basal medium; glucose was selected as the carbon source, with an optimum concentration of 10 g/l, and potassium nitrate was chosen as the nitrogen source, with an optimum concentration of 1.9 g/l. Under optimum conditions, Haematococcus sp. demonstrated an increase in biomass concentration from 0.18 gDW/l to 5.58 gDW/l in 14 days, after which there was a 31-fold increase in its growth. At the same time, the concentrations of chlorophyll and carotenoids were 172.16 mg/l and 42.33 mg/l, respectively. This work will contribute to the basic data for mass cultivation of microalgae.
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